Manufacturing method of artificial begonia fimbristipula hance seed

A technology of G. japonica and artificial seeds, which is applied in horticultural methods, botanical equipment and methods, gardening tools/equipment, etc., can solve problems such as shortage of wild resources of G. japonica, and achieves overcoming low efficiency and solving survival rate. low effect

Inactive Publication Date: 2010-07-14
ZHAOQING UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for making artificial seeds of G. chinensis, which can effectively solve the problem of the increasing shortage of wild resources of G. chinensis, meet the needs of people in their lives, and have easy access to materials and technological process. Simple, not affected by seasons, fast reproduction, high survival rate, low cost

Method used

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  • Manufacturing method of artificial begonia fimbristipula hance seed
  • Manufacturing method of artificial begonia fimbristipula hance seed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment one: the making of artificial seed of G. violet-backed

[0033] 1. Induction of bulbs of G. purple-backed

[0034] Wash and sterilize the fresh leaves of wild G. chinensis, cut them into 1cm×1cm pieces, and inoculate them on the bulb induction medium under a sterile environment. The medium composition is MS+3% sucrose+ 0.7% agar + hormone. Cultivate under the conditions of 23±1°C, 16 light per day, and 1600 Lux light intensity, and differentiate into bulbs after 30 days.

[0035] 2. Use the corm of G. violet back as the artificial seed propagule, use MS + 2% sodium alginate + hormone + 3% sucrose as the artificial endosperm component that is the embedding agent, 2% sodium alginate + 0.5-1.5g / L CaCl 2 It is the artificial seed coat matrix, that is, the artificial seeds are obtained, and then placed on MS medium to germinate.

[0036] details as follows:

[0037] (1) MS (Murashige and SKoog) ingredients:

[0038] Macroelements: KNO 3 1900mg / L, NH 4 NO 3...

Embodiment 2

[0055] Embodiment two: the comparative experiment of the artificial seed of G. violet-backed

[0056] In the preparation method of G. violet-backed artificial seed of the present invention, the hormone selected in the embedding agent (artificial endosperm) composition is 6-BA and GA 3 , contribute to plant rooting and germination, in order to explore the role of the two hormones in the artificial seed germination, the following comparative experiments were carried out, and the results are shown in Table 1:

[0057] Table I

[0058] serial number

[0059] It can be seen from the above table that in Experimental Examples 2 and 3, 6-BA and GA in the hormone contained in the embedding agent 3 When the concentration is 1.5mg / L, the effect on the germination of artificial seeds alone is similar. In Experimental Example 4, 6-BA and GA in the embedding agent hormone 3 The concentration is 0.5mg / L, and the effect is not very obvious. In Experimental Example 5, the concent...

Embodiment 3

[0060] Embodiment three: the comparative experiment of the artificial seed of G. violet-backed

[0061] Example 2 shows that in artificial seeds, changes in the hormone concentration of the embedding agent can cause changes in seed germination. The purpose of this example is to explore the CaCl in the artificial seed coat matrix 2 Whether the change of concentration also affects the germination of artificial seeds, the following experiment was carried out: after the corms and embedding solution were fully mixed, in the ultra-clean workbench, use tweezers to clamp the corm-containing embedding solution into 1.0% CaCl 2 , carry out the ion exchange reaction for 15-30 minutes. The results are shown in Table 2

[0062] Table II

[0063] serial number

[0064] serial number

[0065] After the bulb and embedding solution are fully mixed, use tweezers to clamp the bulb-containing embedding solution into 1.5% CaCl in an ultra-clean workbench 2 , carry out the i...

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Abstract

The invention belongs to the technical field of production of artificial plant seeds, and relates to a manufacturing method of an artificial begonia fimbristipula hance seed. The specific steps are as follows: establishment of a begonia fimbristipula hance bulb induction system, embedment of an artificial seed coat of the artificial begonia fimbristipula hance seed in a substrate, and preparation of an artificial embryosperm and an artificial seed germination substrate, thereby opening a novel way for large-scale artificial cultivation of begonia fimbristipula hance. The invention solves the problem of begonia fimbristipula hance seed sources and avoids the damage to wild plant resources, the materials can be easily obtained, the process flow is simple, the manufacturing method can not be influenced by seasons, the propagation speed is high, and the survival rate is also high.

Description

technical field [0001] The invention belongs to the technical field of plant artificial seed production, and relates to a method for making artificial seeds of geranium purple-backed. Background technique [0002] Begonia fimbristipula Hance, according to "Flora of China", is a Begoniaceae plant, distributed in Guangdong, Guangxi, Fujian and Jiangxi in my country, and the type specimens were collected from Dinghu Mountain and Luofu Mountain in Guangdong (Flora of China ( Volume 52, Volume 1) 1999, 190-192). It can be used as a traditional Chinese medicine, internally used for heatstroke fever, lymphatic tuberculosis, lung heat cough, hemoptysis, pneumonia, chronic bronchitis, sore throat. Externally used for blood stasis, abdominal pain, sprains, fractures, burns, etc. At the same time, geranium is also rich in red pigment, which is used to develop a variety of geranium drinks and foods, such as geranium tea, geranium soft candy, geranium drink, etc. Various products with ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 陈雄伟陈刚王瑛华梁少玲
Owner ZHAOQING UNIV
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