Method for breeding entomopathogenic nematodes

A technology of entomopathogenic nematodes and Tenebrio molitor, which is applied in animal husbandry and other fields, can solve the problems of slow reproduction speed, low output and high cost of entomopathogenic nematodes, and achieve the goal of saving water, electricity and labor, high yield and survival rate, and low cost Effect

Inactive Publication Date: 2010-07-21
SHANDONG INST OF POMOLOGY
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Problems solved by technology

[0004] In order to solve the problems of slow reproduction speed, high cost and low yield of entomopathogenic nematodes, the present invention p

Method used

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  • Method for breeding entomopathogenic nematodes
  • Method for breeding entomopathogenic nematodes

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Embodiment Construction

[0019] The present invention will be further described below with tree sawdust as embodiment:

[0020] The entomopathogenic nematode breeding method steps are as follows:

[0021] (1) drying or drying tree sawdust to make 100 grams of dry sawdust as plant debris, and then using sterilization equipment to carry out high-pressure moist heat sterilization of the dry sawdust;

[0022] (2) The treated sawdust is allowed to cool, then add 200 grams of water and stir evenly;

[0023] (3) Put the sawdust after adding water into a transparent glass bottle as a culture container, and its volume accounts for 1 / 2 of the capacity of the glass bottle; Liquid, the suspension concentrate contains 15,000 live entomopathogenic nematodes per milliliter, that is, 500 entomopathogenic nematodes are added for every 1 gram of live Tenebrio molitor;

[0024] (4) Seal the glass bottle, leave a gap at the mouth of the bottle to maintain ventilation, and then place it in a culture room with a temperat...

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Abstract

The invention discloses a method for breeding entomopathogenic nematodes. The method is realized in a way that: a transparent culture container is used as a breeding container, a live yellow mealworm is used as a host insect, phytodetritus is used as a culture medium, a sterilizing device is used for sterilization, and a temperature-control culture room is used for culturing. The invention obviously solves the problem of waste pollution of plant straws and wood, greatly reduces the production cost for the raw materials and simplifies the procedures for production and use. The cultured entomopathogenic nematodes have the advantages of low cost, good quality and good effects for prevention and control, and can be widely used for preventing and controlling subterranean pest-insect and concealed insects of plants in farm land, forests, gardens and the like.

Description

technical field [0001] The invention relates to a breeding method of nematodes, which belongs to the field of biological breeding and cultivation. Background technique [0002] Entomopathogenic nematodes are nematodes specialized in parasitic pests. They have a wide range of hosts, can actively seek hosts, are safe and non-toxic to humans, animals and the environment, and can be artificially cultivated in large quantities. They can effectively control agricultural, forestry, and horticultural plants. Boring pests, such as peach borer, various grubs, beetles, cutworms, etc., are therefore called high-potential biological control weapons by experts at home and abroad. [0003] The propagation of entomopathogenic nematodes is mainly divided into in vivo propagation and in vitro artificial medium propagation, and the in vitro artificial medium propagation is divided into solid culture and liquid culture. Both methods require a variety of raw materials and large-scale equipment, ...

Claims

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Application Information

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IPC IPC(8): A01K67/033
Inventor 孙瑞红李爱华李晓军辛力张勇王涛
Owner SHANDONG INST OF POMOLOGY
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