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Regulation and control fragment related to arsenic response and application thereof

A fragment and purpose technology, applied in the field of regulatory fragments, can solve problems such as ambiguity

Inactive Publication Date: 2012-05-09
INST OF BOTANY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, how the expression of these genes is regulated is still unclear

Method used

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  • Regulation and control fragment related to arsenic response and application thereof
  • Regulation and control fragment related to arsenic response and application thereof
  • Regulation and control fragment related to arsenic response and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1. Obtaining of Regulatory Fragments and Their Functional Detection

[0036] 1. Structural feature analysis of the promoter regulatory sequence of the Arabidopsis arsenate reductase gene AtACR2 and the design of the promoter regulatory expression vector of the Arabidopsis AtACR2 gene

[0037] 1. Structural characteristics analysis of the promoter regulatory sequence of Arabidopsis arsenate reductase gene AtACR2

[0038] According to the Arabidopsis genome sequence, the genome of the AtACR2 gene consists of three exons and two introns ( figure 1 ). Among them, the first exon has 164 bases (including 33bp 5' non-coding region), and the second exon has 139 bases. There are 2 introns (406bp and 511bp respectively) after such a short exon fragment, so it is speculated that the expression regulation region of the gene is in addition to the promoter upstream of the 5' non-coding region, and its 2 introns region may also be involved in the expression regulation of th...

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PUM

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Abstract

The invention discloses a regulation and control fragment related to arsenic response and an application thereof. The nucleotide sequence of the regulation and control fragment is 1) the nucleotide sequence shown by the 1-1856th place from the 5' end of sequence 1 in a sequence table, or 2) the nucleotide sequence which is hybridized with the nucleotide sequence in 1) under high rigorous condition and is provided with promoter activity, or 3) the nucleotide sequence having more than 70% of homology with the nucleotide sequence in 1) and having the promoter activity. Experiment proves that theregulation and control fragment participates in arsenic response and has the promoter activity.

Description

technical field [0001] The invention relates to a regulatory segment related to arsenic response and application thereof. Background technique [0002] With the development of industry, the pollution of heavy metals to the environment has become a global problem. Among them, the pollution of arsenic to soil and water occurs from time to time in our country. Only from 2008 to the beginning of 2009, six incidents of arsenic pollution occurred consecutively in Dushan County of Guizhou, Chenxi County of Hunan, Hechi of Guangxi, Yangzonghai of Yunnan, Dasha River of Henan, and the Picang flood diversion at the junction of Shandong and Jiangsu, resulting in more than Thousands of people were poisoned by arsenic in varying degrees. The harm of arsenic is similar to that of other heavy metals in that it cannot be decomposed by microorganisms and can accumulate in organisms, and eventually cause harm to humans through the food chain. [0003] Arsenic pollution is not only harmful ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/113C12N15/63C12N5/10C12N1/15C12N1/19C12N1/21A01H5/00G01N33/68G01N21/64
Inventor 徐文忠麻密戴晓静孙洋洋何振艳
Owner INST OF BOTANY CHINESE ACAD OF SCI