Quantitative detection device and detection method of biological fluid samples

Abstract

The invention discloses a biological fluid sample quantitative detection device, which comprises a test strip arranged in a box, a memory for color intensity data and a reader for the color intensity data; the bottom layer of the test strip is a substrate, and the substrate is provided with It has a porous test main membrane; a second membrane is arranged upstream of the main test membrane, and a sample application site and a label ligand site are arranged on the second membrane, and the biological fluid sample and its complex to be tested are promoted by capillary action , the labeled ligand can flow; the downstream of the main test membrane is provided with a third membrane, and the third membrane is used to absorb the biological fluid sample to be tested that has passed the test main membrane at the end of the downstream of the main test membrane; the box cover is provided with There are two sample injection holes and a viewing window, and the detection device of the present invention can use the light reflection intensity of the known concentration standard strip to draw a standard curve, so as to perform rapid, effective and accurate quantitative analysis on biological fluid samples.

Description

technical field [0001] The invention relates to a system for detecting biological fluid samples, in particular to a method for detecting and quantitatively measuring the concentration of analyte in the sample. Background technique [0002] Quantitative analysis of antigens, hormone-like antibodies, endocrine proteins, and other types of proteins often provides crucial clinical data. In the biological field, immunoassays are well known. The principle is that after the reagents are impregnated, they are capillary-action on the membrane containing the immobilized reagents, and the visible markers, such as latex particles and metal complexes, are bound to the detection zone of the test strip. reaction of the analyte. It is worth mentioning that the label is obtained from the metal sol, and the label processed from the metal sol can be combined with the ligand, and then further combined with the analyte, ligand or antibody. Test strips have been used extensively for the quantit...

AI Technical Summary

Problems solved by technology

[0005] In the prior art, most of the devices for determining the presence or absence of the analyte based on the test strip cannot provide the accurate amount of the analyte in the liquid sample.

Even if the detection device is equipped with a quantitative test standard, it will be inaccurate due to temperature, air humidity, time lapse, test strip differences, and signal intensity changes

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