Method for producing sophorolipid by continuous feeding and fermentation

A technology of fed-batch fermentation and sophorolipids, which is applied in the field of fermentation to produce sophorolipids, can solve the problems of unfavorable promotion and application of sophorolipids, low yield of sophorolipids, and large process loss rate, etc., and achieve high practical application value, The effect of shortening the fermentation cycle and improving production quality

Inactive Publication Date: 2010-09-29
天津实发中科百奥工业生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the fermentation cycle is longer, the production process is more complicated, and the loss rate of the post-extraction process is larger, so the yield of sophorolipids

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0016] Example 1

[0017] (1) Preparation of spore suspension: Inoculate Saccharomyces sphaeroides TJZKBA10326 on the slant of the culture medium, cultivate at a constant temperature of 30°C for 2 days, add normal saline with a mass concentration of 0.75% to wash the mycelium to obtain a bacterial suspension and suspend it. The spore content in the liquid is about 3.6×10 5 One, to be used; the slant medium composition includes (1L): glucose 8g, malt powder 3g, peptone 4g, yeast powder 4g, agar 15g, distilled water to 1L, pH 3.5. The strain used is: Torulopsis bombicola TJZKBA10326.

[0018] (2) The above bacterial suspension is used to inoculate the sterilized liquid seed culture medium. The composition of the liquid seed culture medium includes (1L): fructose 30g, corn oil 30g, yeast powder 5g, urea 0.5g, and distilled water to make the volume constant To 1L, pH 3.5. Sterilize the liquid seed culture medium at 121°C for 15 minutes and then cool it to room temperature; then add t...

Example Embodiment

[0024] Example 2

[0025] (1) Preparation of spore suspension: inoculate Saccharomyces sphaeroides TJZKBA10326 on the slope, incubate at a constant temperature of 30°C for 2 days, add normal saline with a mass concentration of 0.75% to wash the mycelium, and obtain a bacterial suspension. The spore content is about 4.5×10 5 One, to be used; the slant medium composition includes (1L): glucose 8g, malt powder 3g, peptone 4g, yeast powder 4g, agar 15g, distilled water to 1L, pH 3.5.

[0026] (2) The above bacterial suspension is used to inoculate the sterilized liquid seed culture medium. The composition of the liquid seed culture medium includes (1L): 50g sucrose, 50g rapeseed oil, 7g yeast powder, 0.8g urea, fixed with distilled water Volume to 1L, pH 3.5. Sterilize the liquid seed medium at 121°C for 15 minutes and then cool it to room temperature; then add the spore suspension to the above-mentioned cooled liquid seed medium according to the volume ratio of 4% of the inoculum (th...

Example Embodiment

[0031] Example 3

[0032] (1) Preparation of spore suspension: Inoculate Saccharomyces coccidius TJZKBA10326 on the slope, culture at 30°C for 3 days, add normal saline with a mass concentration of 0.75% to wash the mycelium to obtain a bacterial suspension. Spore content is about 5.8×10 5 One, to be used; the slant medium composition includes (1L): glucose 8g, malt powder 3g, peptone 4g, yeast powder 4g, agar 15g, distilled water to 1L, pH 3.5.

[0033] (2) The above bacterial suspension is used to inoculate the sterilized liquid seed culture medium. The composition of the liquid seed culture medium includes (1L): glucose 60g, soybean oil 60g, yeast powder 8g, urea 1g, distilled water to make the volume to 1L, pH 3.5. Sterilize the liquid seed medium at 121°C for 15 minutes and then cool it to room temperature; then add the spore suspension to the above-mentioned cooled liquid seed medium according to the volume ratio of 3% of the inoculum (the amount of liquid in a 500ml shake b...

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Abstract

The invention relates to a method for producing sophorolipid by continuous feeding and fermentation, including the following steps: (1) preparation of spore suspension: torulopsosis TJZKBA10326 is inoculated onto a slope and cultured at 30 DEG C for 2-4 days, and 0.75% physiological saline is added to wash mycelium, so as to obtain fungus suspension, 105-106 spores are contained in suspension; (2) the fungus suspension is used for being inoculated into liquid seed culture medium after sterilization, so as to obtain liquid seed; (3) the liquid seed culture medium is used for being inoculated into a 5L fermentation tank after sterilization to be fermented; (4) in the time period of 24-72h of fermentation, oil with the concentration of 30-60g/L is fed every 12h; in the fermentation process, glucose with the concentration of 30-60g/L is fed, so as to control the concentration of glucose to be 5-10g/L; dissolved oxygen is 20-30%, pH value is 3.3-3.8; (5) after fermentation for 72h, mixed organic solvent ethyl acetate/isopropanol with the same volume of fermentation liquid, ratio of 4:1 and unit of v/v is used for extracting sophorolipid, and vacuum-pumping method is used for removing organic solvent, and yield of sophorolipid is 200-240g/L. The method shortens fermentation period and improves production efficiency and yield.

Description

technical field [0001] The invention relates to a method for fermenting and producing sophorolipids, in particular to a method for continuously feeding fermented and producing sophorolipids. Background technique [0002] Sophorolipid is a biodegradable biosurfactant. Biosurfactants have high selectivity, and their surface activity is less affected by conditions such as extreme temperature, pH value, and salinity. Compared with other surfactants produced by chemical synthesis or petroleum refining, biosurfactants have broad application prospects in the fields of food, cosmetics, medicine and environmental protection. As one of the important members, sophorolipids have low toxicity, good compatibility with the human body and the environment, and have good emulsification, dispersion, and solubilization properties. They are widely used in food, medicine, cosmetics, metallurgy, environmental protection, and waste oil recovery. The field has research and development value, and h...

Claims

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Application Information

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IPC IPC(8): C12P19/12C12N1/16C12R1/88
Inventor 徐勇虎刘晓鸥国华李睿颖王永乐胡娅君刘云
Owner 天津实发中科百奥工业生物技术有限公司
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