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Fluorescence-based pipette instrument

A technology of pipettes and pipette tips, which is applied to instruments, scientific instruments, and laboratory containers, etc., and can solve the problems of time-consuming counting cells and waste of resources

Inactive Publication Date: 2010-10-27
EI SPECTRA LLC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Unfortunately, counting cells using this method under a microscope is time-consuming and resource-intensive, and the calculation accuracy is all dependent on the number of cells the user is willing to actually count in a given volume

Method used

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  • Fluorescence-based pipette instrument
  • Fluorescence-based pipette instrument
  • Fluorescence-based pipette instrument

Examples

Experimental program
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Embodiment Construction

[0033] specific implementation plan

[0034] Reference will now be made to the drawings in which the various elements of the invention will be given numerical designations and in which the invention will be discussed to enable one of ordinary skill in the art to make and use the invention. It is to be understood that the ensuing description is merely exemplary of the principles of the invention and should not be taken as narrowing the scope of the appended claims.

[0035] As generally used in this disclosure, unless otherwise obvious from the context, the term "fluid" may include a liquid alone, one or more liquids in a mixture, or one or more liquids and carried or suspended in particles in it. In certain instances, the liquid will have electrolytic properties. A bulk fluid container is simply a container sized to hold a sufficient quantity of fluid to form at least one fluid sample to be interrogated using embodiments of the present device.

[0036] The term "particle" a...

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Abstract

An improved pipette tip (276) including an elongate body stretching between a proximal end (277) and a distal end. The body typically includes a plurality of thin film layers (e.g. 154, 156, 102, 158, 202) configured and arranged to provide a fluid path extending from the distal end toward the proximal end (277). The improved pipette tip (276) includes an interrogation zone in which to interrogate fluid flowing along the fluid path. One operable interrogation arrangement, generally (100), includes structure configured to permit detection of radiation resulting from a Stokes-shift. Optionally,a sensor component may include one or more electrode (e.g. 248, 250) that is disposed in the fluid path to contact fluid therein for electrically-based interrogation. A pipette tip (276) may be embodied to: count particles, verify sample integrity (e.g. freedom from bubbles), monitor sample flow rate, and confirm an inspired volume, among other uses.

Description

[0001] priority claim [0002] This application claims priority on the filing date of U.S. Provisional Patent Application No. 61 / 004,630, filed November 27, 2007, for "Fluorescence-based pipette instrument", the entire contents of which Incorporated herein by reference. technical field [0003] The present invention relates to devices for extracting a fluid sample from a bulk fluid container for subsequent or simultaneous optical and / or electrical interrogation of the sample. Background technique [0004] Hand-held pipettes are used for precise fluid volume measurement and delivery and are some of the most ubiquitous and widely used laboratory tools available to scientists today. Many procedures requiring small-volume fluid handling of biological and chemical liquid samples rely on their ease of use, precision, and repeatability to ensure accurate, consistent experimental handling. Many fixed-volume and adjustable-volume pipettes are commercially available. When used in l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/02
CPCB01L2300/0654B01L2200/0689B01L2200/0647G01N2021/6482B01L2300/0645G01N2035/1039G01N2035/1062G01N21/645B01L3/0275G01N15/10G01N21/05G01N2021/0346B01L2300/025B01L3/0217G01N21/65G01N2015/1062B01L2300/0887G01N2015/1486B01L2300/0874B01L3/50273G01N35/10G01N2035/103G01N2015/1024
Inventor H·E·埃里夫C·S·金
Owner EI SPECTRA LLC
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