Method for preparing gold nano particles and application of particles in detection of cyhalothrin

The technology of gold nanoparticles and pyrethroid is applied in the field of nano-optics, which can solve the problems of long detection time, complicated operation and high cost of chromatography technology, and achieve the effects of simple and quick method, simple process and easy operation.

Inactive Publication Date: 2010-11-24
HUAZHONG NORMAL UNIV
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Problems solved by technology

Chromatography is considered to be the most classic and commonly used pesticide residue detection technology, such as gas chromatography, liquid chromatography, gas chromatography-mass spectrometry, liquid chromatography-mas

Method used

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  • Method for preparing gold nano particles and application of particles in detection of cyhalothrin
  • Method for preparing gold nano particles and application of particles in detection of cyhalothrin
  • Method for preparing gold nano particles and application of particles in detection of cyhalothrin

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preparation example Construction

[0025] 1. Preparation of mercaptoethylamine-modified gold nanoparticles

[0026] The present invention adopts the sodium borohydride reduction method, that is, gold perchlorate is used as a gold source, mercaptoethylamine is used as a stabilizer, and twice distilled water is used as a reaction solvent, and a one-pot reaction is used to prepare mercaptoethylamine-modified gold nanoparticles, and the specific preparation The method is: see Figure 6 , at room temperature, in 94mL water, add 1mL 10 -2 mol / L AuClO 4 Aqueous solution, 4mg sodium citrate, stir for 1-5 minutes, add 4-12mg NaBH 4 , continue stirring for 0.5-2 hours, add 0.5-5mL 3×10 -5 mol / L aqueous solution of mercaptoethylamine. The maximum absorption peak of the gold nanometer is at 520nm, and the particle diameter is about 8nm, and the properties do not change within one month (see figure 1 ).

[0027] Such as figure 2 Shown: 0.25mL 10 -4 The mol / L kofatethrin and kungfuthrin analogue solutions were added...

Embodiment 1

[0031] Example 1, the detection of kaufthrin in pure aqueous solution by mercaptoethylamine-modified gold nanoparticles:

[0032] 0.25mL of different concentrations (5×10 -5 ~10 -8 mol / L) of kaufthrin solution was added to 1.5mL of mercaptoethylamine-modified gold nanoparticles, and mixed evenly. After 5 minutes, the color of the solution changed from wine red to purple black. After 10 minutes, UV-Vis absorption spectrum analysis was carried out respectively, see Figure 4 , the absorption intensity at 520nm gradually decreases, the peak shape becomes wider, and a new peak gradually appears at 580nm. Its colorimetric detection limit is 5×10-8 mol / L. Among them, the concentrations of kungfuthrin are: b1, 0; b2, 5×10 -5 ;b3, 1×10 -5 ;b4, 5×10 -6 ;b5, 1×10 -6 ;b6, 5×10 -7 ;b7, 1×10 -7 ;b8, 5×10 -8 ;b9,1×10 -8 .

Embodiment 2

[0033] Example 2, the detection of kuerthrin in lake water by mercaptoethylamine-modified gold nanoparticles:

[0034] Take 100ml of lake water, add 20mg of sodium carbonate, oscillate fully, if necessary, oscillate in an ultrasonic cleaner for 5 minutes, mix well, then heat and boil for half an hour, and finally filter twice with ordinary filter paper for later use. With the lake water that has been processed, kaufthrin is made into different concentrations of solutions (5 × 10 -5 ~10 -8 mol / L). Take 0.25mL of different concentrations of kungfuthrin solution and add it to 1.5mL of mercaptoethylamine-modified gold nanometer solution, mix well, and the concentration after 5 minutes is not less than 1×10 6 The color of the solution changed from wine red to purple blue. After 10 minutes, UV-visible absorption spectrum analysis was carried out, such as Figure 5 As shown, the absorption intensity at 520nm gradually decreases, the peak shape becomes wider, and a new peak gradua...

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Abstract

The invention provides a method for preparing gold nano particles. The method particularly comprises the following steps of: at room temperature, adding perchloric acid gold into distilled water to obtain 10<-2>mol/L aqueous solution of the perchloric acid gold; adding sodium citrate and sodium borohydride while stirring the aqueous solution; continuously stirring the mixed solution for 0.5 to 2 hours; and adding 3*10<-5>mol/L aqueous solution of mercaptoethylamine to obtain mercaptoethylamine-modified gold nano particles, wherein the weight ratio of the distilled water, the aqueous solution of the perchloric acid gold, the sodium citrate, the sodium borohydride to the aqueous solution of the mercaptoethylamine is 1:(0.008-0.01): (0.032-0.04):(0.04-0.12):(0.005-0.05). The method is simple and has high operability; and by the change of visible color of the gold nano particles prepared by the method, cyhalothrin in esbiothrin pesticides can be quickly identified and detected, and the detection limit reaches national standard value.

Description

technical field [0001] The invention relates to the field of nanometer optics. Through the special optical properties of gold nanometers, mercaptoethylamine-modified gold nanoparticles are used as probes, which are widely used in analysis and detection work. Background technique [0002] Pyrethroid pesticides are widely used because of their broad-spectrum, low toxicity, high efficiency, and biodegradability. However, there are residues in crops, soil, and water bodies. Varieties have teratogenic and mutagenic effects on humans. Therefore, the residues and detection of pyrethroid pesticides in the environment have always been a concern of people. However, the existing literatures on the detection of pyrethroid residues in environmental samples are all focused on chromatographic methods. Chromatographic techniques are considered to be the most classic and commonly used pesticide residue detection techniques, such as gas chromatography, liquid chromatography, gas chromatogra...

Claims

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Application Information

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IPC IPC(8): C09K9/00G01N21/78G01N21/33
Inventor 李海兵李玉玲田德美
Owner HUAZHONG NORMAL UNIV
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