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Method for extracting polar or medium polar plant source natural product
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A technology of natural products and plant sources, which is applied in the field of natural product extraction, can solve the problems of high production cost, significant degradation of target objects, and high requirements, and achieve the effects of increased yield, shortened extraction time, and reduced emissions
Active Publication Date: 2012-08-15
CHINA AGRI UNIV +1
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Problems solved by technology
However, existing studies have found that microwave, ultrasonic, and high-voltage pulsed electric field technologies can significantly degrade the target substances during the extraction process, while supercritical fluid technology is mainly aimed at weakly polar and non-polar substances in natural products, and ultra-high pressure technology has a significant impact on the extraction process. The equipment requirements are high, the minimum processing pressure is 100MPa, and the production cost is relatively high
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Examples
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Effect test
Embodiment 1
[0023] Extraction of anthocyanins
[0024] The plant matrix rich in natural anthocyanins such as purple cabbage is broken into silk, the purple cabbage silk is placed in a nylon bag, put into a HPCD reactor preheated to 40°C, water is added as an extraction solvent, and the reactor is sealed. The air inlet is boosted to 15MPa, the carbon dioxide diffuses to the outlet of the reactor through the solid-liquid mixture, passes through the filter at the outlet, and enters the air inlet again through the circulation pipeline. The volume ratio of the solid-liquid mixture to the high-pressure carbon dioxide is 1:5 , the volume of high-pressure carbon dioxide is controlled by fixing the volume of the reactor and the volume of the solid-liquid mixture of purple cabbage. After dynamic treatment for 15 minutes, the pressure is released to release the anthocyanin extract. Quantitative analysis, microbial index determination and color evaluation were carried out on it.
[0025] The yield o...
Embodiment 2
[0028] Extraction of anthocyanins
[0029] The extraction temperature is 60° C., the pressure is 5 MPa, the volume ratio of the solid-liquid mixture to high-pressure carbon dioxide is 4:5, and the extraction time is 5 min. The remaining specific operations are the same as in Example 1.
[0030] Compared with the control experiment, the purple cabbage anthocyanin yield obtained in this embodiment increased by 10%.
[0031] The definition of the control test is the same as in Example 1.
Embodiment 3
[0033] Extraction of anthocyanins
[0034] The extraction temperature was 90° C., the pressure was 45 MPa, the volume ratio of the solid-liquid mixture to high-pressure carbon dioxide was 4:5, and the extraction time was 60 minutes. The remaining specific operations were the same as in Example 1.
[0035] Compared with the control experiment, the anthocyanin yield of purple cabbage obtained by this method is increased by 8%.
[0036] The definition of the control test is the same as in Example 1.
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Abstract
The invention discloses a method for extracting a polar or medium polar plant source natural product, which comprises the following steps of: crushing a plant source substance containing the polar or medium polar plant source natural product, then adding water into the crushed plant source substance, raising the pressure to between 5 and 50MPa at the temperature of between 40 and 90 DEG C, and circularly introducing high-pressure carbon dioxide to treat the mixture for 1 to 60 minutes. The method can be used for extraction of the polar natural product such as anthocyanin, and also can be usedfor extraction of other polar and medium polar natural products.
Description
technical field [0001] The invention relates to a method for extracting natural products, in particular to a method for extracting natural products by using high pressure carbon dioxide technology (High Pressure Carbon Dioxide, HPCD). Background technique [0002] Generally speaking, solvent extraction is often used for natural product extraction, that is, according to the solubility of the target substance in the solvent, a solvent with high solubility for the target substance, insoluble or low solubility for the non-target substance is selected, and then the target substance is selectively extracted. The method of dissolution of substances from the matrix and into the solvent. Solvent extraction method is divided into water method and organic solvent method. Since the target object is generally divided into two types: hydrophilic and lipophilic, organic solvent method can be further divided into hydrophilic organic solvent method (such as ethanol, methanol, acetone method)...
Claims
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Application Information
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