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Method for improving material blood compatibility by using electrostatic self-assembled coating

An electrostatic self-assembly and hemocompatibility technology, applied in the field of surface science and biomedical polymer materials, can solve the problems of difficult to obtain phosphorylcholine-rich surfaces, limit biocompatibility, limit applications, etc. Achieve the effects of reduced platelet adhesion, improved blood compatibility, and strong adaptability

Inactive Publication Date: 2012-05-23
NORTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the grafting method is difficult to obtain a surface rich in phosphorylcholine groups, which also limits the further improvement of biocompatibility, and the grafting modification is affected by conditions such as equipment, substrate composition, and shape, which limits the use of Applications in the field of surface modification of biomaterials (Langmuir, 2009, 25(6): 3610-3617; Science, 1997, 277(5330): 1232-1237; Biomacromolecules, 2009, 10(8): 2275-2283; Biomacromolecules, 2007, 8(10): 3169-3176)

Method used

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  • Method for improving material blood compatibility by using electrostatic self-assembled coating
  • Method for improving material blood compatibility by using electrostatic self-assembled coating
  • Method for improving material blood compatibility by using electrostatic self-assembled coating

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 0.5 g of chitosan was dissolved in 100 mL of 1% acetic acid aqueous solution, and stirred at room temperature for 24 hours. After the chitosan is dissolved, filter it with a sand-core funnel and let it stand for 30 minutes to eliminate air bubbles. After the air bubbles were eliminated, the chitosan film was coated on the glass slide by dip coating, and left to air at room temperature for 24 hours. After the chitosan film is dried, soak it in 0.1% sodium hydroxide solution for 1 hour, then soak it with distilled water, change the distilled water every 4-5 hours, and soak for 48 hours in total. Finally, vacuum-dry at 30°C for 8 hours to obtain a chitosan film.

Embodiment 2

[0028] Get the chitosan film that above-mentioned method prepares, be soaked in 20mL, in 1mg / mL PMA30, PMA50, PMA70 aqueous solution (30,50,70 represent respectively the percent mole percent of monomer MPC feeding in polymer synthesis and account for total throwing monomer content), adsorbed for 15min, then rinsed with distilled water, and freeze-dried to obtain a modified chitosan film. Compared with the chitosan membrane, the advancing angle of the modified membrane in contact with water was reduced by about 20 degrees.

Embodiment 3

[0030] Take the chitosan film prepared by the above method, soak it in 20mL, 1mg / mL PMA30, 0.14M NaCl aqueous solution, absorb for 15min, rinse with 0.14M NaCl aqueous solution and distilled water successively, and freeze-dry to obtain the modified chitosan membrane. sugar film. Compared with the chitosan membrane, the modified membrane significantly reduced the amount of protein adsorption, platelets did not aggregate and had only a small amount of adhesion, and the blood compatibility was significantly improved ( figure 1 ).

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Abstract

The invention discloses a method for improving material blood compatibility by using an electrostatic self-assembled coating. The method comprises that: polyanion dipolymer is adsorbed on the surface of a polycation material by electrostatic interaction, and a cell outer membrane simulating structure is formed on the surface of the polycation material. The binary copolymer of acrylic monomers containing amphoteric ions and negative ions is taken as polyanions, the polycations and the polyanions construct the cell outer membrane simulating structure on the surface of the polycation material byelectrostatic self-assembly, and the polyanion dipolymer is adsorbed on the surface of the polycations by electrostatic interaction, so the method has simple condition and strong adaptability and canrealize surface modification of the polycation material with a complex shape structure. Compared with the polycation material, the protein adhesion and platelet adherence of the modified surface are obviously reduced, and the blood compatibility is remarkably improved.

Description

technical field [0001] The invention relates to a method for improving blood compatibility of materials by using an electrostatic self-assembly coating, and belongs to the technical fields of surface science and biomedical polymer materials. Background technique [0002] Gene therapy is considered to be an effective way to treat various genetic diseases (including infectious diseases, cancer, etc.). In the process of gene therapy, gene vectors (including viral vectors and non-viral vectors) must be used to deliver nucleic acid drugs to the lesion site. The former is a carrier with high transfection efficiency, but the immune response and gene mutation limit its application. Therefore, people are paying more and more attention to safe, low-toxic non-viral vectors, among which polycationic gene vectors are one of the research hotspots in recent years. [0003] In practical applications, polycationic non-viral gene carriers also have certain problems. Specifically, the surfa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/32A61K47/36A61L27/34A61L31/10A61L33/06A61L33/08
Inventor 宫铭张辉宫永宽
Owner NORTHWEST UNIV
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