33 results about "Cell outer membrane" patented technology

The invention discloses a method for improving material blood compatibility by using an electrostatic self-assembled coating. The method comprises that: polyanion dipolymer is adsorbed on the surface of a polycation material by electrostatic interaction, and a cell outer membrane simulating structure is formed on the surface of the polycation material. The binary copolymer of acrylic monomers containing amphoteric ions and negative ions is taken as polyanions, the polycations and the polyanions construct the cell outer membrane simulating structure on the surface of the polycation material by electrostatic self-assembly, and the polyanion dipolymer is adsorbed on the surface of the polycations by electrostatic interaction, so the method has simple condition and strong adaptability and can realize surface modification of the polycation material with a complex shape structure. Compared with the polycation material, the protein adhesion and platelet adherence of the modified surface are obviously reduced, and the blood compatibility is remarkably improved.

The invention discloses a super-hydrophilic and underwater super-lipophobic coating which consists of a substrate and an organic / inorganic hybrid coating, wherein the organic / inorganic hybrid coating containing an ampholytic ionic group is coated on the surface of the substrate. 1, the method disclosed by the invention is simple in process, easy in preparation of raw materials, simple in equipment and low in cost, and the functional coating can be prepared on the surfaces of different substrates in a large area, so as to achieve super hydrophilicity and underwater super lipophobicity; and 2, by introducing a betaine-type ampholytic ionic compound of a cell outer membrane simulating molecular structure to the prepared super-hydrophilic and underwater super-lipophobic coating, the prepared functional coating not only has functions of self-cleaning, anti-fogging, underwater oil resistance and the like, but also has excellent performances of resisting biological adhesion and the like.

The invention discloses a preparation method of an aldehyde-containing phosphorylcholine polymer and dopamine cross-linked coating. The method comprises the steps of 1, preparing an aldehyde-containing phosphorylcholine polymer; 2, dissolving the dopamine and the aldehyde-containing phosphorylcholine polymer in a polar solvent to obtain a mixed solution, coating the mixed solution onto the surface of a to-be-modified material, and drying the to-be-modified material; 3, placing the dried to-be-modified material in an alkaline aqueous solution, and heating the material at 40 DEG C to 90 DEC C for 2-12 hours to obtain a cross-linked adhesive bionic coating of a cell outer membrane-simulated structure. The preparation method is simple and convenient in operation, which provides a novel approach for preparing a stable surface with the cross-linked adhesive bionic coating of the cell outer membrane-simulated structure. The prepared coating has a wide application prospect in the fields of blood purification, in-vivo implantation materials, tissue engineering, drug sustained release, biological sensors and the like.

The invention belongs to the field of veterinary preparation, and relates to a veterinary preparation namely lysozyme-loaded chitosan microspheres and a preparation method thereof. The chitosan with a molecular weight of 5 to 50 kDa is crosslinked with an electronegative crosslinking agent through the electrostatic action so as to form microspheres, and the lysozyme is encapsulated in the microspheres. The ion-crosslinking method and the freeze-drying method are co-used to prepare the veterinary preparation, the reaction conditions are mild, the technology process is controllable, the industrialization development becomes easier, and thus the preparation method is practical. The lysozyme and chitosan can generate a synergetic effect, the chitosan with a molecular weight of 5-50 kDa can penetrate the outer membrane of G- cells, and thus the lysozyme can be transferred to the inner wall layer of G- cells to hydrolyze the peptidoglycan structure, so that the inhibiting performance of lysozyme on Gram-negative bacteria (G-) is effectively strengthened, and the preparation can partially replace the broad-spectrum antibacterial effect of antibiotics.

The invention discloses a synthesis and coating method of a double bionic dopamine and phosphorylcholine substance. The synthesis and coating method comprises the following steps of enabling a vinyl monomer containing a phosphorylcholine group and dopamine hydrochloride to generate Michael addition reaction in a polarity solvent at certain temperature, so as to obtain the double bionic dopamine and phosphorylcholine substance; thinning a reaction solution, coating the surface of the to-be-modified material, airing, heating at high temperature, treating by a Tris-HCl solution, adhering to the surface of a substrate, and building a bionic cell outer membrane structure. A preparation method of a coating with the bionic cell outer membrane structure has the advantages that the preparation method is simple, and the conditions are mild; a novel path is provided for obtaining a stable phosphorylcholine bionic coating, and the important academic meaning is realized for modifying the biological compatibility of the biological material. A modifying material with the bionic cell outer membrane structure has broad application prospect in the fields of in-vivo implant materials, tissue engineering, medicine slow release, biological sensors and the like.

The invention relates to a method for preparing red cell outer membrane protein of left- -eyed flounders, Paralichthys olivaceus, comprising the following steps: blood is taken from the caudal vein of the left-eyed flounder; the Alsever gelation inhibitor and the peripheral blood of the left-eyed flounder are mixed; the blood is diluted by PBS buffer solution, mixed with separating medium and centrifugated at the temperature of 4 DEG C, and the deposit formed by the red cells at the bottom is centrifugally washed at the temperature of 4 DEG C by the PBS buffer solution to remove supernatant fluid; hypotonic buffer solution is added and kept at the temperature of 4 DEG C to fully swell the red cell so that the membrane is ruptured; the deposit of the red cell membrane of the left-eyed flounder is centrifugally collected at the temperature of 4 DEG C; the hypotonic buffer solution is added and then the deposit of the red cell membrane is scattered by a whirlpool mixer and centrifugally washed at the temperature of 4 DEG C; membrane protein lysis solution is added and stirred to fully cleave the red cell membrane of the left-eyed flounder; after centrifugating at the temperature of 4 DEG C, the supernate is the extracting solution of the red cell membrane protein of the left-eyed flounder; after being subpackaged, the extraction solution is preserved in an refrigerator at the temperature of minus 80 DEG C. Compared with the prior art, the method is simpler and more efficient, thereby not only ensuring the high efficiency of the preparation of membrane protein, but also maintaining the biological activity of the membrane protein.

The invention discloses a phosphoryl choline polymer containing aldehyde group, and the polymer is a binary copolymer composite synthesized of Vinyl monomers containing aldehyde group and Vinyl monomers containing phosphorus acyl choline through radical polymerization. Furthermore, the invention discloses the preparation method and the application of the polymer. The polymer is used for coating the surface of to-be-decorated material after being mixed with polyamino material, the reaction of the aldehyde group and the amidogen in the coating layer are controlled by temperature, so as to enable the density of the coating layer to be increased, and achieve the purpose of fixing phosphoryl bile bases on the surface of the material and obtain a coating layer with a structure imitating of cell outer membrane. The protein absorption and blood platelet adhesion of the coating layer are remarkably decreased, the biocompatibility is remarkably improved, and the phosphoryl choline polymer has great academic values and broad application prospects in the filed of tissue engineering, drug controlled release, gene therapy, biosensor and the like.

The invention discloses a method of improving fermentation cell density using hemoglobin and provides a method of improving microorganism fermentation cell density. According to the method, by expressing hemoglobin in periplasmic space or displaying it by cell surface display, and by rebuilding cell outer membranes, contact of the hemoglobin with oxygen is facilitated, thus improving oxygen utilization and improving microorganism fermentation density. Experiments show that an engineering bacterium built by the invention can increase microorganism fermentation cell density and content of intracellular accumulated cells PHB.

The invention discloses a preparation method for an imitation cell outer membrane structure coating with a surface of phosphorylcholine. The preparation method includes the steps of performing simplesolution free radical polymerization on a vinyl monomer containing an amphoteric ion phosphorylcholine group and a vinyl monomer containing an amino group, so as to synthesize a phosphorylcholine polymer containing an amino group, uniformly mixing the phosphorylcholine polymer and glutaraldehyde in a polar solvent, coating the surface of a material to be modified with the obtained solution, and then performing grafting on the phosphorylcholine polymer containing the amino group, so as to obtain the imitation cell outer membrane structure coating with the surface of high-density phosphorylcholine. The preparation method is simple and mild in conditions, and provides a new way for obtaining a coating surface with excellent hemocompatibility by increasing the density of phosphorylcholine on the coating surface. The modified material of the imitation cell outer membrane structure is expected to have broad application prospects in the fields of blood purification, in-vivo implantation materials, tissue engineering, drug release, biosensors and the like.

The invention provides a lithium battery cell outer membrane hot melting mechanism. The lithium battery cell outer membrane hot melting mechanism comprises a battery cell loading mechanism and a unloading mechanism arranged in a straight line, a mechanical arm used for grabbing and releasing battery cells, an insulating membrane loading mechanism and a holt melting membrane coating mechanism which are symmetrically arranged on the two sides of a battery cell loading mechanism discharging end, a top pushing gas cylinder arranged right above the hole melting membrane coating mechanism, and an encapsulating mechanism symmetrically arranged on the two sides of a feed inlet end of the unloading mechanism. The lithium battery cell outer membrane hot melting mechanism is capable of completing insulating membrane loading, battery cell loading, insulating membrane bending and packaging, insulating membrane and cover plate part hot melting, insulating membrane encapsulating, and membrane coated battery cell unloading automatically, realizing simultaneous operation of insulating membrane hot melting and encapsulating, and increasing membrane coating efficiency of battery cell insulating membrane effectively.

The invention discloses a composition with a sterilization protecting function and application of the composition and relates to the field of medicine injection. The composition is prepared from the following materials in parts: 1 to 5 parts of chitosan, 1 to 3 parts of chitosan quaternary ammonium salt, 5 to 10 parts of sodium hyaluronate and 0.1 to 1 part of membrane-forming agent. By using thechitosan, the permeability of a cell outer membrane can be improved; a cell membrane is damaged by electrostatic interaction between the chitosan and the cell membrane and finally death of thallus iscaused; the positive ion of the chitosan quaternary ammonium salt is bonded with protein with negative charge in the thallus to form ions, and further the thallus is deactivated. Helicobacter pylori and candida albicans in stomach can be efficiently killed, stay time of the chitosan composition in intestines and stomach can be prolonged and the effect of prolonging the pharmaceutical effect of thechitosan is realized.

The invention discloses feed for reducing shell rot disease of sea shrimps as well as a preparation method and application of the feed. The feed consists of fish oil, purple potato powder, shrimp shell powder, phospholipid oil, superoxide dismutase SOD, a dandelion root extract, an antioxidant, a mildew inhibitor, a hylocereus undatus extract, an epimedium herb extract, neutral protease, carotenoid, polygonum multiflorum vine extract, choline chloride, a herba houttuyniae extract, betaine, a medicated leaven extract and a fructus lycii extract. The feed is difficult to get mildew and go bad, and caking and odor smells are avoided; and the feed is high in stability in water, cannot be scattered in 4 hours to 5 hours, and has no residues and side effects; pH of intestinal tracts of the sea shrimps can be kept at 5 to 7, vibrio cell outer membranes are damaged, vibrio parahaemolyticus, aeromonas, myxobacteria and flavobacterium can be killed, survival ratios of the sea shrimps are increased by 15 percent to 25 percent, the anti-stress capability of the sea shrimps is improved, the proliferation capability is improved, the palatability of feed is good, a digestion ratio is increased, the growth speed of the sea shrimps is high, the body length is increased by 20 percent to 50 percent, and the sea shrimps are 29cm to 36cm long.

The invention discloses a preparation method of Escherichia coli inner membrane vesicles of endogenous high-expression nucleic acid anti-tumor drugs. The "tRNA scaffold method" is used to stably insert miRNA into a carrier, and then the carrier is transformed into E. coli for use. It endogenously expresses a large amount of miRNA with the function of killing cancer cells, uses lysozyme to remove the outer membrane and periplasmic components of E. coli, and obtains E. coli protoplasts, uses polycarbonate membrane to filter the protoplasts, and breaks the protoplasts. Finally, the protoplast inner membrane vesicles were purified and separated by ultracentrifugation, and the inner membrane vesicles of low-toxic Escherichia coli with high expression of miRNA were obtained. The method provided by the invention has the advantages of simple operation, low production cost, large-scale fermentation and preparation, low toxicity and high efficiency. As a new type of drug carrier, it can obviously inhibit the growth of non-small cell lung cancer when it is applied in the preparation of antitumor drugs, and has broad application prospects in the field of drug carriers.

The invention discloses a synthesis and coating method of a double bionic dopamine and phosphorylcholine substance. The synthesis and coating method comprises the following steps of enabling a vinyl monomer containing a phosphorylcholine group and dopamine hydrochloride to generate Michael addition reaction in a polarity solvent at certain temperature, so as to obtain the double bionic dopamine and phosphorylcholine substance; thinning a reaction solution, coating the surface of the to-be-modified material, airing, heating at high temperature, treating by a Tris-HCl solution, adhering to the surface of a substrate, and building a bionic cell outer membrane structure. A preparation method of a coating with the bionic cell outer membrane structure has the advantages that the preparation method is simple, and the conditions are mild; a novel path is provided for obtaining a stable phosphorylcholine bionic coating, and the important academic meaning is realized for modifying the biological compatibility of the biological material. A modifying material with the bionic cell outer membrane structure has broad application prospect in the fields of in-vivo implant materials, tissue engineering, medicine slow release, biological sensors and the like.

The invention discloses a phosphoryl choline polymer containing aldehyde group, and the polymer is a binary copolymer composite synthesized of Vinyl monomers containing aldehyde group and Vinyl monomers containing phosphorus acyl choline through radical polymerization. Furthermore, the invention discloses the preparation method and the application of the polymer. The polymer is used for coating the surface of to-be-decorated material after being mixed with polyamino material, the reaction of the aldehyde group and the amidogen in the coating layer are controlled by temperature, so as to enable the density of the coating layer to be increased, and achieve the purpose of fixing phosphoryl bile bases on the surface of the material and obtain a coating layer with a structure imitating of cell outer membrane. The protein absorption and blood platelet adhesion of the coating layer are remarkably decreased, the biocompatibility is remarkably improved, and the phosphoryl choline polymer has great academic values and broad application prospects in the filed of tissue engineering, drug controlled release, gene therapy, biosensor and the like.

The present invention involves a antibacterial drug hydrochloride hydrochloric acid composite liposome and preparation method. It uses the synergistic effect of levofloxacin hydrochloric acid hydrochloric acid, and the effect of levofloxacin hydrochloride hydrochloride hydrochloride and octumi is significantly enhanced.Neviliated bacteria, the outer outer leaf layer is mainly composed of LPS. The internal liquidity is extremely low. It can effectively exclude some antibiotics. After the hydrochloric acid left oxygen and mixture are made into composite lipids, lipids can effectively effectivelyElberry on the wall of E. coli, destroys the production of the cell wall, invades the outer membrane, and enters the interior of the bacteria by combining with the outer membrane to achieve a better bacteriostatic effect.

The invention discloses a functional polymer containing phosphorylcholine and polyethylene glycol and a method for constructing an anti-biological pollution interface. The polymer is a functional polymer with a variety of functional groups in the side chain and a controllable ratio: the polyethylene glycol (PEG) flexible chain with a large steric hindrance and the phosphorylcholine hydrophilic group on the outer membrane of the cell work together Achieve good anti-fouling effect; the polymer contains less than 10% catechol groups, which can be firmly combined with the surface of various substrates mediated by polydopamine to form a stable hydrophilic monomolecular coating, realizing the material Functional modification of the surface. The method for forming the coating in the invention is simple, can exist stably on the surface of any substrate, and improves the ability of the material surface to resist the adhesion and pollution of various biological components.

The invention discloses a method for improving material blood compatibility by using an electrostatic self-assembled coating. The method comprises that: polyanion dipolymer is adsorbed on the surface of a polycation material by electrostatic interaction, and a cell outer membrane simulating structure is formed on the surface of the polycation material. The binary copolymer of acrylic monomers containing amphoteric ions and negative ions is taken as polyanions, the polycations and the polyanions construct the cell outer membrane simulating structure on the surface of the polycation material byelectrostatic self-assembly, and the polyanion dipolymer is adsorbed on the surface of the polycations by electrostatic interaction, so the method has simple condition and strong adaptability and canrealize surface modification of the polycation material with a complex shape structure. Compared with the polycation material, the protein adhesion and platelet adherence of the modified surface are obviously reduced, and the blood compatibility is remarkably improved.

The invention provides a biological material for enhancing electron transfer efficiency as well as a preparation method and application of the biological material. The biological material is prepared by mixing a recombinant strain containing a TtGH8 gene, a recombinant strain containing an SxA gene, a recombinant strain containing an FsUA gene, a recombinant strain containing an XylB gene and an XylC gene, and a recombinant strain containing an XylA gene and an XylD gene, and then mixing with a reaction substrate. Exogenous genes related to hemicellulose degradation and xylose degradation are expressed in escherichia coli, different anchoring protein genes are selected at the same time, hemicellulose and xylose degradation gene expression products are anchored to an extracellular membrane, and electron transfer can be achieved without a substrate entering bacteria. The degradation process of the biological material is accompanied by the generation of NADH and electrons, so that the effect of enhancing the electron transfer efficiency is achieved.

The invention relates to a method for densifying a bionic coating of amino-acid-containing phosphorylcholine polymers and glutaraldehyde. The amino-acid-containing phosphorylcholine polymers are synthesized from vinyl monomers containing zwitter-ion phosphorylcholine groups and vinyl monomers containing amino acid through simple solution free radical polymerization and then uniformly mixed with glutaraldehyde in a polar solvent, the mixture is applied to the surface of a material required to be modified and then grafted with the vinyl monomers containing the zwitter-ion phosphorylcholine groups, and a bionic cell outer membrane structure coating with high surface phosphorylcholine density can be obtained. The preparation method is simple and uses mild conditions, and a new way is provided to improve the surface phosphorylcholine density of the coating, so that a coating surface with excellent blood compatibility is obtained. The modified material adopting a bionic cell outer membrane structure has broad application prospects in the fields such as blood purification, in-vivo implantation of materials, tissue engineering, sustained drug release, biosensors and the like.

The invention discloses a preparation method for an imitation cell outer membrane structure coating with a surface of phosphorylcholine. The preparation method includes the steps of performing simplesolution free radical polymerization on a vinyl monomer containing an amphoteric ion phosphorylcholine group and a vinyl monomer containing an amino group, so as to synthesize a phosphorylcholine polymer containing an amino group, uniformly mixing the phosphorylcholine polymer and glutaraldehyde in a polar solvent, coating the surface of a material to be modified with the obtained solution, and then performing grafting on the phosphorylcholine polymer containing the amino group, so as to obtain the imitation cell outer membrane structure coating with the surface of high-density phosphorylcholine. The preparation method is simple and mild in conditions, and provides a new way for obtaining a coating surface with excellent hemocompatibility by increasing the density of phosphorylcholine on the coating surface. The modified material of the imitation cell outer membrane structure is expected to have broad application prospects in the fields of blood purification, in-vivo implantation materials, tissue engineering, drug release, biosensors and the like.

The invention discloses a method of improving fermentation cell density using hemoglobin and provides a method of improving microorganism fermentation cell density. According to the method, by expressing hemoglobin in periplasmic space or displaying it by cell surface display, and by rebuilding cell outer membranes, contact of the hemoglobin with oxygen is facilitated, thus improving oxygen utilization and improving microorganism fermentation density. Experiments show that an engineering bacterium built by the invention can increase microorganism fermentation cell density and content of intracellular accumulated cells PHB.

Compositions and methods are provided for making and using a cell having expressed on its outer plasma membrane surface an Anchor designed to form a first complex with a selected Linker. The Linker is designed to form a second complex with in a target protein that is not recognized by the Anchor. These cells can be primary cells or immortalized cells, they function as fusion partners to create hybridoma cells. The cells can be designed to bind a secreted targeted protein, e.g., a secreted antibody, to the cell surface via an immune complex formed by the Anchor-Linker and permit the identification of the existence, antigen specificity, and antigen binding affinity, titer, amount, or biological activity of the target protein. In certain embodiments, the Linker is of a species heterologous to the species of the Anchor; and the target protein is of a species heterologous to the Linker and to the Anchor.

The invention discloses klebsiella pneumoniae bacteriophage lyase with an amino acid sequence as shown in SEQ ID No.1. According to the klebsiella pneumoniae bacteriophage lyase disclosed by the invention, a lyase coding gene is cloned from klebsiella pneumoniae bacteriophage DP, and a prokaryotic expression mode is adopted to induce expression of dissolvable lyase; the obtained lyase recombinant protein can penetrate through an extracellular membrane of gram-negative bacteria under the condition of no outer membrane penetrating agent, hydrolyzes a peptidoglycan layer of a bacterial cell wall, shows strong bacteriostatic activity on gram-negative bacteria such as pseudomonas aeruginosa and escherichia coli, can be used for preparing drugs for resisting gram-negative bacteria, and can be used for preparing drugs for resisting gram-negative bacteria. Powerful support is provided for treating and inhibiting gram-negative bacterial diseases; in addition, the preparation method of the lyase is simple and suitable for industrial large-scale production.

The invention provides a small-ring type DNA (deoxyribonucleic acid) recombinant vector capable of realizing eukaryotic excrine expression of HIV (human immunodeficiency virus)-1 membrane fusion inhibition protein HR212, which comprises a nucleotide sequence coding HR212 and a small-ring DNA vector. The invention also provides a small-ring type DNA gene therapeutic agent for blocking an HIV-1 membrane fusion process; the small-ring type DNA gene therapeutic agent comprises a small-ring type DNA recombinant vector capable of realizing eukaryotic excrine expression of HIV-1 membrane fusion inhibition protein HR212; the active ingredient is nucleotide coding heptad repeat triple-helix protein HR212; and after transfecting eukaryotic cells through a small-ring type DNA vector mode, the nucleotide is expressed in the cells and is released outside the cells through an excrine or cell outer membrane expression mode, the process of virus entering a host cell is blocked, and then an effect on suppressing HIV-1 virus infection is realized. The invention also provides an application of the small-ring type DNA recombinant vector coding heptad repeat triple-helix protein HR212 in preparing a gene therapeutic agent for resisting HIV-1 infection.