33 results about "Cell outer membrane" patented technology

The invention belongs to the field of veterinary preparation, and relates to a veterinary preparation namely lysozyme-loaded chitosan microspheres and a preparation method thereof. The chitosan with a molecular weight of 5 to 50 kDa is crosslinked with an electronegative crosslinking agent through the electrostatic action so as to form microspheres, and the lysozyme is encapsulated in the microspheres. The ion-crosslinking method and the freeze-drying method are co-used to prepare the veterinary preparation, the reaction conditions are mild, the technology process is controllable, the industrialization development becomes easier, and thus the preparation method is practical. The lysozyme and chitosan can generate a synergetic effect, the chitosan with a molecular weight of 5-50 kDa can penetrate the outer membrane of G- cells, and thus the lysozyme can be transferred to the inner wall layer of G- cells to hydrolyze the peptidoglycan structure, so that the inhibiting performance of lysozyme on Gram-negative bacteria (G-) is effectively strengthened, and the preparation can partially replace the broad-spectrum antibacterial effect of antibiotics.

The invention discloses a synthesis and coating method of a double bionic dopamine and phosphorylcholine substance. The synthesis and coating method comprises the following steps of enabling a vinyl monomer containing a phosphorylcholine group and dopamine hydrochloride to generate Michael addition reaction in a polarity solvent at certain temperature, so as to obtain the double bionic dopamine and phosphorylcholine substance; thinning a reaction solution, coating the surface of the to-be-modified material, airing, heating at high temperature, treating by a Tris-HCl solution, adhering to the surface of a substrate, and building a bionic cell outer membrane structure. A preparation method of a coating with the bionic cell outer membrane structure has the advantages that the preparation method is simple, and the conditions are mild; a novel path is provided for obtaining a stable phosphorylcholine bionic coating, and the important academic meaning is realized for modifying the biological compatibility of the biological material. A modifying material with the bionic cell outer membrane structure has broad application prospect in the fields of in-vivo implant materials, tissue engineering, medicine slow release, biological sensors and the like.

The invention relates to a method for preparing red cell outer membrane protein of left- -eyed flounders, Paralichthys olivaceus, comprising the following steps: blood is taken from the caudal vein of the left-eyed flounder; the Alsever gelation inhibitor and the peripheral blood of the left-eyed flounder are mixed; the blood is diluted by PBS buffer solution, mixed with separating medium and centrifugated at the temperature of 4 DEG C, and the deposit formed by the red cells at the bottom is centrifugally washed at the temperature of 4 DEG C by the PBS buffer solution to remove supernatant fluid; hypotonic buffer solution is added and kept at the temperature of 4 DEG C to fully swell the red cell so that the membrane is ruptured; the deposit of the red cell membrane of the left-eyed flounder is centrifugally collected at the temperature of 4 DEG C; the hypotonic buffer solution is added and then the deposit of the red cell membrane is scattered by a whirlpool mixer and centrifugally washed at the temperature of 4 DEG C; membrane protein lysis solution is added and stirred to fully cleave the red cell membrane of the left-eyed flounder; after centrifugating at the temperature of 4 DEG C, the supernate is the extracting solution of the red cell membrane protein of the left-eyed flounder; after being subpackaged, the extraction solution is preserved in an refrigerator at the temperature of minus 80 DEG C. Compared with the prior art, the method is simpler and more efficient, thereby not only ensuring the high efficiency of the preparation of membrane protein, but also maintaining the biological activity of the membrane protein.

The invention discloses a preparation method for an imitation cell outer membrane structure coating with a surface of phosphorylcholine. The preparation method includes the steps of performing simplesolution free radical polymerization on a vinyl monomer containing an amphoteric ion phosphorylcholine group and a vinyl monomer containing an amino group, so as to synthesize a phosphorylcholine polymer containing an amino group, uniformly mixing the phosphorylcholine polymer and glutaraldehyde in a polar solvent, coating the surface of a material to be modified with the obtained solution, and then performing grafting on the phosphorylcholine polymer containing the amino group, so as to obtain the imitation cell outer membrane structure coating with the surface of high-density phosphorylcholine. The preparation method is simple and mild in conditions, and provides a new way for obtaining a coating surface with excellent hemocompatibility by increasing the density of phosphorylcholine on the coating surface. The modified material of the imitation cell outer membrane structure is expected to have broad application prospects in the fields of blood purification, in-vivo implantation materials, tissue engineering, drug release, biosensors and the like.

The invention provides a biological material for enhancing electron transfer efficiency as well as a preparation method and application of the biological material. The biological material is prepared by mixing a recombinant strain containing a TtGH8 gene, a recombinant strain containing an SxA gene, a recombinant strain containing an FsUA gene, a recombinant strain containing an XylB gene and an XylC gene, and a recombinant strain containing an XylA gene and an XylD gene, and then mixing with a reaction substrate. Exogenous genes related to hemicellulose degradation and xylose degradation are expressed in escherichia coli, different anchoring protein genes are selected at the same time, hemicellulose and xylose degradation gene expression products are anchored to an extracellular membrane, and electron transfer can be achieved without a substrate entering bacteria. The degradation process of the biological material is accompanied by the generation of NADH and electrons, so that the effect of enhancing the electron transfer efficiency is achieved.