Klebsiella pneumoniae bacteriophage lyase as well as preparation method and application thereof

A technology of Klebsiella phage and lyase, which is applied in the field of bioengineering, can solve problems such as hindering the contact between lyase and peptidoglycan, and achieve the effect of simple preparation method
CN114807104APending Publication Date: 2022-07-29SOUTHWEST UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SOUTHWEST UNIV
Publication Date
2022-07-29

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Abstract

The invention discloses klebsiella pneumoniae bacteriophage lyase with an amino acid sequence as shown in SEQ ID No.1. According to the klebsiella pneumoniae bacteriophage lyase disclosed by the invention, a lyase coding gene is cloned from klebsiella pneumoniae bacteriophage DP, and a prokaryotic expression mode is adopted to induce expression of dissolvable lyase; the obtained lyase recombinant protein can penetrate through an extracellular membrane of gram-negative bacteria under the condition of no outer membrane penetrating agent, hydrolyzes a peptidoglycan layer of a bacterial cell wall, shows strong bacteriostatic activity on gram-negative bacteria such as pseudomonas aeruginosa and escherichia coli, can be used for preparing drugs for resisting gram-negative bacteria, and can be used for preparing drugs for resisting gram-negative bacteria. Powerful support is provided for treating and inhibiting gram-negative bacterial diseases; in addition, the preparation method of the lyase is simple and suitable for industrial large-scale production.
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Description

technical field

[0001] The invention belongs to the technical field of bioengineering, and relates to a Klebsiella pneumoniae phage lyase, a preparation method thereof, and application in the preparation of medicines. Background technique

[0002] Phage lyases are unique to double-stranded DNA bacteriophages, and are a class of cell wall hydrolases synthesized during the late stage of viral replication. It can reach the peptidoglycan target on the cell wall through the hole formed by perforin in the cell membrane, and cleave and hydrolyze the important chemical bonds in the peptidoglycan, eventually leading to bacterial lysis and death. Phage lyase can selectively and quickly kill specific bacteria, and it is not easy to develop resistance, and does not destroy the normal flora of the body. It is a "precise" antibacterial substance.

[0003] The research on lyase for Gram-positive bacteria has made satisfactory progress, but the research on lyase for Gram-negative bacteria ...

Claims

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