A kind of preparation method of imitation cell outer membrane structure coating with phosphorylcholine on the surface

A technology that imitates the outer membrane of cells and phosphorylcholine, which is applied to devices, coatings, and prostheses that coat liquid on the surface, which can solve the difficulties of application, difficulty in simulating the structure of the outer membrane of cells, and unsatisfactory stability and other problems, to achieve the effect of simple preparation method, broad application prospects, and increased density

Inactive Publication Date: 2019-08-16
XIAN UNIV OF SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The introduction of crosslinkable groups into phosphorylcholine polymers can form a relatively stable coating imitating the outer membrane structure of cells, but the synthesis of phosphorylcholine polymers for building stable coatings is complicated, difficult to apply, and stable Sex is not ideal
In addition, due to the limitation of phosphorylcholine content in the polymer and the internal migration of phosphorylcholine groups on the surface during coating drying and storage, the density of phosphorylcholine groups on the surface of the coating is low, making it difficult to effectively simulate Cell outer membrane structure, blood compatibility needs to be improved

Method used

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  • A kind of preparation method of imitation cell outer membrane structure coating with phosphorylcholine on the surface
  • A kind of preparation method of imitation cell outer membrane structure coating with phosphorylcholine on the surface
  • A kind of preparation method of imitation cell outer membrane structure coating with phosphorylcholine on the surface

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Effect test

Embodiment 1

[0031] Weigh 16mmol 2-methacryloyloxyethylphosphorylcholine and 4mmol 2-aminoethyl methacrylate hydrochloride, use 0.1mmol potassium persulfate as the initiator, and polymerize at 70°C under nitrogen protection After 24 hours, the reaction was dialyzed, and then freeze-dried at -50° C. to obtain an amino group-containing phosphorylcholine polymer PMH20 (20 represents 20% molar ratio of amino group vinyl monomer feed during polymer synthesis). Similarly, samples such as PMH10, PMH15 and PMH25 can be synthesized.

[0032] Using a 400MHz NMR instrument to D 2 O is the hydrogen NMR of the solvent test polymer. There is no peak at 5-7ppm, indicating that there is no residual monomer in the resulting copolymer, and the polymer has been successfully synthesized, with 3.28ppm being -N + (CH 3 ) 3 Characteristic peaks, 0.9-2.2ppm are the peaks of methylene and side chain methyl groups on the main chain to calculate the polymer composition, and it can be known that the polymer composi...

Embodiment 2

[0042] Weigh 17mmol 2-methacryloyloxyethylphosphorylcholine and 3mmol 2-aminoethyl methacrylate hydrochloride, use 0.1mmol potassium persulfate as the initiator, and polymerize at 70°C under nitrogen protection After 24 hours, the reaction was dialyzed, and then freeze-dried at -50°C to obtain an amino group-containing phosphorylcholine polymer.

[0043] The amino group-containing phosphorylcholine polymer and glutaraldehyde in this embodiment are dissolved in an ethanol solvent in a molar ratio of 100:5 according to the amino group and the aldehyde group. After mixing uniformly, the concentration of the amino group-containing phosphorylcholine polymer is 0.5mg / mL, coating 10μl / cm 2 / surface on the chitosan material surface to dry, and then the methanol solution of 2mg / mL PMH20 coating volume 5 microliters / cm 2 / surface, dried in the air, and then treated in distilled water at 80 degrees for 12 hours, and then washed with a large amount of methanol and distilled water in tu...

Embodiment 3

[0045] Weigh 12mmol 2-methacryloyloxyethyl phosphorylcholine and 8mmol 2-aminoethyl methacrylate hydrochloride, use 0.1mmol potassium persulfate as the initiator, and polymerize at 70°C under nitrogen protection After 24 hours, the reaction was dialyzed, and then freeze-dried at -50°C to obtain an amino group-containing phosphorylcholine polymer.

[0046] The amino group-containing phosphorylcholine polymer and glutaraldehyde in this embodiment are dissolved in methanol solvent in a molar ratio of 100:15 according to the amino group and the aldehyde group. After mixing uniformly, the concentration of the amino group-containing phosphorylcholine polymer is 2mg / mL, coating 10μl / cm 2 / surface on the chitosan material surface to dry, and then the methanol solution of 3mg / mL PMH15 coating volume 20 microliters / cm 2 / surface, dried in the air, and then treated in distilled water at 85 degrees for 11 hours, and then washed with a large amount of methanol and distilled water in tur...

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Abstract

The invention discloses a preparation method for an imitation cell outer membrane structure coating with a surface of phosphorylcholine. The preparation method includes the steps of performing simplesolution free radical polymerization on a vinyl monomer containing an amphoteric ion phosphorylcholine group and a vinyl monomer containing an amino group, so as to synthesize a phosphorylcholine polymer containing an amino group, uniformly mixing the phosphorylcholine polymer and glutaraldehyde in a polar solvent, coating the surface of a material to be modified with the obtained solution, and then performing grafting on the phosphorylcholine polymer containing the amino group, so as to obtain the imitation cell outer membrane structure coating with the surface of high-density phosphorylcholine. The preparation method is simple and mild in conditions, and provides a new way for obtaining a coating surface with excellent hemocompatibility by increasing the density of phosphorylcholine on the coating surface. The modified material of the imitation cell outer membrane structure is expected to have broad application prospects in the fields of blood purification, in-vivo implantation materials, tissue engineering, drug release, biosensors and the like.

Description

technical field [0001] The invention belongs to the technical fields of material surface science and biomedical polymer materials, and in particular relates to a preparation method of a membrane imitating cell outer membrane structure coating whose surface is phosphorylcholine. Background technique [0002] Chitosan has the advantages of degradability, antibacterial, non-toxic, non-irritating, and pH responsiveness (Carbohydrate Polymers 2010, 79:724-730), and has been widely used in biomedicine and other fields. More and more studies have shown that chitosan and its derivatives can be used for blood purification. The amino group on the chitosan molecule helps to adsorb various toxins in the blood, and can be used as a hemoperfusion material (Chemical Journal of Chinese Universities, 2002, 23:75-77; Journal of Microencapsulation 1993, 10:475-486). Chitosan membrane has high dialysis rate, selectivity and strength, and can be used as hemodialysis material (Journal of Applied...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J7/04C08L5/08C08F230/02C08F220/34C09D143/02C09D7/63A61L27/34A61L27/50B05D7/24
CPCA61L27/34A61L27/50B05D7/24C08F220/34C08F230/02C08J7/042C08J2305/08C08J2443/02C08K5/07C08L5/08C09D143/02
Inventor 宫铭
Owner XIAN UNIV OF SCI & TECH
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