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A gene carrier imitating cell outer layer membrane structure and its preparation method

A technology imitating the outer membrane of cells and structural genes, which is applied in the field of nanotechnology and biomedical polymer materials, can solve the problems of low density of phosphorylcholine groups and limit biocompatibility, and achieve platelet adhesion reduction, Effect of biocompatibility improvement and load capacity improvement

Inactive Publication Date: 2016-04-13
XIAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these modification methods all adopt the approach of phosphorylcholine small molecule grafting (Carbohydrate Polymers, 2007, 70 (1): 82-88; ZL200410054022.2; Biomacromolecules, 2007, 8 (10), 3169-3176; Biomacromolecules, 2006, 7 (11): 3151-3156; Journal of Applied Polymer Science, 2003, 88 (2): 489-493; Polymer International, 2003, 52 (1): 81-85; Journal of biomaterials science, Polymeredition, 2002, 13 (5): 501-510; Colloids and Surfaces B: Biointerfaces, 2009, 71(2): 268-274), often the density of phosphorylcholine groups on the surface of the material is not high, and the graft modification is affected by the composition, shape and equipment of the substrate impact, which limits its application in the field of biomedical material modification and further improvement of biocompatibility

Method used

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  • A gene carrier imitating cell outer layer membrane structure and its preparation method
  • A gene carrier imitating cell outer layer membrane structure and its preparation method
  • A gene carrier imitating cell outer layer membrane structure and its preparation method

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Embodiment 1

[0030]The imitation cell outer membrane structure gene carrier of the present embodiment is made by free radical polymerization of 2-methacryloyloxyethyl phosphorylcholine (MPC), aniline and dendritic polyurethane, wherein the monomer (2-methyl The mass of 2-methacryloyloxyethylphosphorylcholine and aniline is 60% of the total mass of monomer and polycation (dendritic polyurethane), and the mass ratio of 2-methacryloyloxyethylphosphorylcholine to aniline It is 2:1.

[0031] The preparation method of this embodiment is: dissolving 0.1g of dendritic polyurethane in 50mL of acetic acid aqueous solution with a concentration of 1% by volume, stirring for 24h to obtain a dendritic polyurethane solution; then adding 0.1g of 2-methyl Acryloyloxyethylphosphorylcholine (MPC), 0.05g aniline and 5mg potassium persulfate (K 2 S 2 o 8 , KPS), under the protection of nitrogen, the polymerization reaction was carried out at a temperature of 70 °C for 4 hours; after the polymerization react...

Embodiment 2

[0034] The imitation cell outer membrane structure gene carrier of the present embodiment is made by free radical polymerization of 2-methacryloyloxyethyl phosphorylcholine (MPC), aniline and chitosan, wherein the monomer (2-methyl The mass of 2-methacryloyloxyethylphosphorylcholine and aniline is 65% of the total mass of monomer and polycation (chitosan), and the mass ratio of 2-methacryloyloxyethylphosphorylcholine to aniline It is 4:1.

[0035] The preparation method of this embodiment is: dissolving 0.35g chitosan in 50mL of 5% acetic acid aqueous solution by volume percentage concentration, stirring for 24h to obtain a chitosan solution; then adding 0.52g 2-methyl Acryloyloxyethylphosphorylcholine, 0.13g aniline and 0.02g potassium persulfate (K 2 S 2 o 8 , KPS), under the protection of nitrogen, the polymerization reaction was carried out at a temperature of 80°C for 3h; after the end of the polymerization reaction, the reaction system was suction filtered with a G3 f...

Embodiment 3

[0038] The imitation cell outer membrane structure gene carrier of the present embodiment is made by free radical polymerization of 2-methacryloyloxyethyl phosphorylcholine (MPC), aniline and chitosan, wherein the monomer (2-methyl The mass of 2-methacryloyloxyethylphosphorylcholine and aniline is 70% of the total mass of monomer and polycation (chitosan), and the mass ratio of 2-methacryloyloxyethylphosphorylcholine to aniline is 6:1.

[0039] The preparation method of the present embodiment is: dissolving 0.3g chitosan in 50mL of 3% aqueous acetic acid solution by volume, stirring for 12h to obtain a chitosan solution; then adding 0.6g 2-methyl Acryloyloxyethylphosphorylcholine (MPC), 0.1g of aniline and 5mg of potassium persulfate (K 2 S 2 o 8 , KPS), under the protection of nitrogen, the temperature is 70 ° C under the conditions of polymerization for 3 hours; after the end of the polymerization reaction, filter the reaction system with a G3 funnel to obtain the filtrat...

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Abstract

The invention discloses a cellulosa membrane structure-like gene carrier which is prepared from monomers and polycation by using a radical polymerization method, wherein the monomers comprise a vinyl monomer and an aniline monomer containing zwitter-ion hydrophilia groups; the polycation is chitosan, polyethyleneimine, polylysine or arborization polyurethane. In addition, the invention further discloses a preparation method of the cellulosa membrane structure-like gene carrier. The particle size of the cellulosa membrane structure-like gene carrier is small, namely, about 300-600nm, the surface Zeta potential is high, and the carrying capability of the carrier to DNA (Deoxyribonucleic Acid) is remarkably improved. The protein adsorption and the blood platelet adhesion of the cellulosa membrane structure-like gene carrier are greatly reduced, the biocompatibility is greatly improved, the in-vivo circulation time of the gene carrier is prolonged, and the medicine effect is improved.

Description

technical field [0001] The invention belongs to the technical field of nanotechnology and biomedical polymer materials, and in particular relates to a gene carrier imitating the outer layer membrane structure of cells and a preparation method thereof. Background technique [0002] Gene therapy is the replacement or supplementation of defective genes in patients with normal functional genes, so as to achieve the purpose of treating diseases. It has become an effective way to treat various genetic diseases (including infectious diseases, cancer, etc.). Among the numerous gene carriers, especially the polycationic gene carrier with low toxicity among non-viral gene carriers (Langmuir, 2009, 25(9):5199-5208) has attracted the attention of scholars from all over the world, and has important academic value and huge application prospect. [0003] Chitosan has the advantages of degradability, antibacterial, non-toxic, non-irritating, and pH responsiveness (Carbohydrate Polymers, 20...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/34A61K48/00C08F251/00C08F283/00C08F230/02C08G73/02
Inventor 吴伯华宫铭张永徐金鑫熊善新
Owner XIAN UNIV OF SCI & TECH
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