Culture medium for preparing Bacillamide C by fermenting and shrinking bacillus as well as preparation and application method thereof

A technology of Bacillus atrophaeus and culture medium, which is applied in the field of culture medium and its preparation, and can solve the problems of low content of active substances, limited fishing sponge resources, and lagging development of drug source sponge drugs, etc.

Inactive Publication Date: 2011-01-19
SHANGHAI JIAO TONG UNIV
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Problems solved by technology

Among many marine organisms, sponges are currently one of the marine organisms with the most abundant active substances. People place great hopes on sponge active substances, but the conte

Method used

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  • Culture medium for preparing Bacillamide C by fermenting and shrinking bacillus as well as preparation and application method thereof
  • Culture medium for preparing Bacillamide C by fermenting and shrinking bacillus as well as preparation and application method thereof
  • Culture medium for preparing Bacillamide C by fermenting and shrinking bacillus as well as preparation and application method thereof

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Embodiment Construction

[0019] The embodiments of the present invention are described in detail below. This embodiment is implemented on the premise of the technical solution of the present invention, and detailed implementation methods and specific operating procedures are provided, but the protection scope of the present invention is not limited to the following implementation example.

[0020] The Bacillamide C involved in the following examples is specifically the Bacillus atrophaeus strain (scientific name), and its preservation information / public access channel is: the 16S rRNA sequence of the bacterial strain is completely consistent with the 16S rRNA sequences of multiple strains of Bacillus atrophaeus, and the Bacillus atrophaeus Bacillus is sold in Waseda (Beijing) Biotechnology Development Co., Ltd.

[0021] 1. Preparation of four basic medium.

[0022] 1) Beef extract peptone medium: 10g tryptone, 5g beef extract dissolved in 1L artificial seawater;

[0023] 2) LB medium: 10 tryptone, 5...

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Abstract

The invention discloses a culture medium for preparing Bacillamide C by fermenting and shrinking bacillus as well as preparation and application method thereof, belonging to the technical field of bacteria culture. The culture medium comprises the following components and contents: 3.64g/L of corn starch, 6.0g/L of tryptone, 6.29g/L of calcium carbonate, 4.0g/L of soya peptone, 0.10g/L of aminothiopropionic acid and 0.02g/L of tryptophan, wherein a solvent is artificial seawater. The invention cannot only meet requirement on shake flask experiment, but also can meet requirement on massively preparing Bacillamide C through fermentation of a fermentation tank.

Description

technical field [0001] The invention relates to a culture medium in the technical field of bacterial culture and its preparation, in particular to a culture medium for preparing Bacillamide C by fermenting Bacillus atrophaeus and its preparation and application method. Background technique [0002] Compared with terrestrial organisms, marine organisms live in an environment with harsh conditions such as high salt, high pressure, low temperature, and scarce nutrients. In order to survive, many marine organisms produce some chemical substances with unique structures in the long-term evolution process. A variety of active substances have been found from marine organisms, including anti-tumor compounds, tumor promoters, anti-inflammatory / allergic compounds, antibacterial, anti-viral compounds, biotoxins, anti-parasitic compounds, polyunsaturated fatty acids, etc., and a considerable part of them Active substances are not found in terrestrial organisms. These marine biologicall...

Claims

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Application Information

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IPC IPC(8): C12P21/00C12R1/07
Inventor 金靓婕李志勇张风丽
Owner SHANGHAI JIAO TONG UNIV
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