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Method for producing nisin by continuous fermentation

A technology of nisin and nisin, which is applied in the field of continuous fermentation to produce nisin, which can solve the problems of non-optimal economic stage of operation time, reduced equipment utilization rate, and increased production cost, so as to improve equipment utilization rate , reduce production costs, increase the effect of fermentation yield

Inactive Publication Date: 2012-06-20
LUOYANG QIHONG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the biggest disadvantage of batch fermentation is that a large amount of operation time is in a non-optimal economic stage, resulting in increased production costs, reduced equipment utilization, and frequent sterilization and cooling processes pose more challenges to energy supply.

Method used

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  • Method for producing nisin by continuous fermentation
  • Method for producing nisin by continuous fermentation
  • Method for producing nisin by continuous fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1. Primary seed preparation

[0031] The first-level seed tank is 200 liters, and the filling capacity is 150 liters. The seed medium is prepared according to the following ratio:

[0032] Glucose: 10 g / l,

[0033] Sucrose: 10 g / l,

[0034] Yeast extract: 7 g / l,

[0035] Dipotassium hydrogen phosphate: 12 g / L,

[0036] Corn syrup: 5 g / L,

[0037] Magnesium sulfate: 0.2 g / l

[0038] 5% sodium hydroxide solution to adjust the pH to 7.2,

[0039] Sterilization: 120°C for 30 minutes, cooling to 32°C, differential pressure inoculation seed solution: 2 liters, sterile air to maintain a pressure of 0.2MPa, and culture for 16 hours.

[0040] Second, secondary seed preparation

[0041] The secondary seed tank is 2000 liters, and the filling capacity is 1500 liters. The seed medium is prepared according to the following ratio:

[0042] Glucose: 10 g / l,

[0043] Sucrose: 10 g / l,

[0044] Yeast extract: 7 g / l,

[0045] Corn syrup: 5 g / L,

[0046] Dipotassium hydrogen ph...

Embodiment 2

[0071] 1. Primary seed preparation

[0072] The first-level seed tank is 200 liters, and the filling capacity is 150 liters. The seed medium is prepared according to the following ratio:

[0073] Sucrose: 10 g / l,

[0074] Glucose: 5 g / L

[0075] Yeast extract: 5 g / l,

[0076] Corn syrup: 5 g / L,

[0077] Magnesium sulfate: 0.2 g / l

[0078] Dipotassium hydrogen phosphate: 15 g / L

[0079] 5% potassium hydroxide solution to adjust the pH to 7.0,

[0080] Sterilization: 120°C for 30 minutes, cooling to 32°C, differential pressure inoculation seed solution: 2 liters, sterile air to maintain a pressure of 0.2MPa, and culture for 24 hours.

[0081] Second, secondary seed preparation

[0082] The secondary seed tank is 2000 liters, and the filling capacity is 1500 liters. The seed medium is prepared according to the following ratio:

[0083] Sucrose: 10 g / l,

[0084] Glucose: 5 g / L

[0085] Yeast extract: 5.0 g / l,

[0086] Corn syrup: 5.0 g / L,

[0087] Dipotassium hydrogen ...

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Abstract

The invention provides a method for producing nisin by continuous fermentation, which is characterized by comprising the following steps: transferring streptococcus lactis which is subjected to primary seed cultivation and secondary seed cultivation to a sterile fermentation medium, and controlling the temperature and pH value in the fermentation process; and when the bacterial concentration reaches a specified level, starting biosynthesis of the nisin, releasing fermentation broth from a fermentation tank continuously while replenishing a well-prepared sterile feed supplement medium in the fermentation tank continuously, and lasting for 7 to 15 days for the continuous production technology. In the invention, Lactococcuslactis of the streptococcus lactis is adopted to be subjected to two-stage seed cultivation, a specified fermentation medium formula is used, the feed supplement speed, the pH value and the bacterial concentration in a bioreactor are controlled, and the fermentation broth containing the nisin is released continuously or intermittently, which is used for producing the nisin. The method of the invention can effectively lessen non-optimal economic stages in the fermentation process, thereby realizing the purposes of improvement of fermentation yield, enhancement of device availability, and reduction of production cost for products.

Description

technical field [0001] The invention relates to a method for producing nisin, in particular to a method for continuously fermenting and producing nisin. Background technique [0002] Nisin (Nisin) is derived from a subspecies of lactic acid streptococcus ( Lactococcus lactis ) is a polypeptide antibiotic substance extracted from fermentation products, which is a natural biological food preservative and antibacterial agent. As early as 1928, Rogers and Whittier found that the metabolites of Streptococcus lactis could inhibit the growth of some Gram-positive bacteria. In 1944, Mattick and Hirch found that some lactis streptococci in the serological N group could produce protein antibacterial substances, named N-Inhibitory substance, (N group antibacterial substances, referred to as Nisin). In 1951, Hirsh et al. applied Nisin to food preservation, successfully inhibited the cheese spoilage caused by Clostridium perfringens, and greatly improved the quality of cheese. In 1953...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/02C12R1/46
CPCC07K14/315C12N1/20C07K14/195
Inventor 鲁来政孙远功林永刚周树青高巧玲
Owner LUOYANG QIHONG BIOTECH
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