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Cell counting method based on image identification

A cell counting and image recognition technology, applied in the field of medical laboratory, can solve problems such as the inability to visually evaluate whether the counting results are correct or not, and the inability to count apoptotic cells well

Active Publication Date: 2011-01-19
北京优纳科技有限公司
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Problems solved by technology

The accuracy of this counting method is around ±50 cells. For damaged cells, conjoined cells, and apoptotic cells, it cannot be counted well, and it is impossible to visually evaluate whether the counting results are correct or not.

Method used

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  • Cell counting method based on image identification
  • Cell counting method based on image identification

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Embodiment Construction

[0019] specific implementation plan

[0020] The technical solutions of the present invention will be described in further detail below in combination with preferred embodiments according to the accompanying drawings.

[0021] A cell counting method based on image recognition disclosed by the present invention, the method uses a fully automatic digital slice scanning device to scan cell slides, and counts cells based on image processing and analysis of cell slides; it is characterized in that the The method includes the following steps:

[0022] (1) Make cell slides, fix the cells to be counted in a square area, and place the cell slides on the slide rack scanned by the scanning device (such as figure 2 shown);

[0023] (2) Through the scanning device (such as figure 1 As shown) the motor drive controls the movement of the slide in the three directions of X / Y / Z, through the optical imaging system composed of the objective lens, the lens barrel and the camera, when the slid...

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Abstract

The invention discloses a cell counting method based on image identification. In the method of the invention, by means of computer vision, solid section information is converted into digital image information by a sensor, and the cell counting is finished by image processing. The method of the invention has the excellent expandability and generality, can detect various cells except immunocyte, and can classify the cells in accordance with cell characteristics and the counting accuracy rate is more than 98%.

Description

technical field [0001] The invention belongs to the field of medical laboratory, and more specifically, the invention relates to a method for counting immune cells, that is, by scanning the image of the whole glass slide, the image information of the slide is obtained, and the method of image recognition is used to identify the immune cells, so as to achieve the purpose of automatic counting and morphological analysis. Background technique [0002] CD4 cells are an important immune cell in the human immune system. Since the target of HIV attack is CD4 cells, the test results play an important role in judging the effect of AIDS treatment and the immune function of patients. The CD4 cells of normal adults range from 500 to 1600 per cubic millimeter. The progressive or irregular decline of CD4 cells in HIV-infected persons indicates that the immune system has been seriously damaged. When the CD4 cells are less than 200 per cubic millimeter, Various opportunistic infections or...

Claims

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Application Information

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IPC IPC(8): G01N15/14
Inventor 刘明星李鹏杰郑众喜韩隽
Owner 北京优纳科技有限公司
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