Method for measuring cotinine in body fluid through molecular-imprinting solid-phase extraction-liquid chromatography

A technology of molecular imprinting and liquid chromatography, which is applied in the direction of measuring devices, material separation, and analysis of materials, can solve the problems of adverse effects on the health and environment of operators, consumption of organic solvents, lack of selectivity, etc., and achieve simple, fast and selective operation. Strong, highly sensitive effects

Inactive Publication Date: 2011-02-02
CHINA TOBACCO JIANGXI IND CO LTD
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Problems solved by technology

The LLE process is cumbersome, time-consuming, consumes a large amount of organic solvents, and has adverse effects on the health of the operator and the environm

Method used

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Embodiment

[0024] a. Cotinine molecularly imprinted polymer was prepared by non-covalent synthesis method. Add 1mmol of cotianine, 4mmol of methacrylic acid and 5.6mL of dichloromethane into a 20mL ampoule, mix well, then add 20mmol of ethylene glycol dimethacrylate and 0.24mmol of azobis Isobutyronitrile. Ultrasonic degassing for 10 minutes, followed by nitrogen gas for 5 minutes, sealed, and placed in a 60°C water bath for polymerization for 24 hours. Break the ampoule to take out the blocky polymer, grind and sieve, collect the polymer with a particle size of 25-36 μm, and use acetone to repeatedly settle to remove fine particles. Take 1 g of the obtained particles, put them into a glass chromatography column, wash them with 300 mL of methanol / acetic acid (90:10) (v / v) and 100 mL of methanol, respectively, to elute the template molecules in the imprinted polymer. The polymer particles were dried in an oven at 105°C for 24 hours and stored for future use.

[0025] b. Pack 200mg of p...

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Abstract

The invention relates to a method for measuring the cotinine in a body fluid, in particular to a method for measuring the cotinine in the body fluid through molecular-imprinting solid-phase extraction-liquid chromatography. The method sequentially comprises the following steps of: (1) synthesizing a cotinine molecular-imprinting polymer by using the cotinine as a template; (2) preparing a solid-phase extraction small column by using the synthesized polymer as a solid-phase extraction adsorbent; (3) sequentially leaching the solid-phase extraction small column by adopting an acetonitrile solution containing organic acid and water, alcohol and a buffer solution; (4) concentrating an eluant to dryness, and adding an acetate buffer solution to obtain a detected liquid; and (5) measuring the cotinine in the detected liquid by utilizing high-efficiency liquid chromatography. In the invention, the cotinine in the body fluid is firstly completely extracted and then eluted through solid-phase extraction, thereby realizing the rapid and accurate quantitative analysis of the cotinine thereof.

Description

technical field [0001] The invention relates to a method for determining cotinine in body fluid, in particular to a method for determining cotinine in body fluid by using a synthetic cotinine molecular imprint polymer. Background technique [0002] With the continuous advancement of the global tobacco control campaign, the public is increasingly concerned about smoking and health issues. Cotinine is the main product of nicotine metabolism in the human body. It has the advantages of long half-life and accurate determination from biological fluids. Cotinine in urine, blood and plasma, and vomit is considered to be a specific, highly sensitive, and most credible biological marker that can reflect the amount of nicotine ingested by the human body through smoking or diet. There is a good positive correlation between the degree of exposure to tobacco smoke and the concentration of cotinine detected in body fluids of smokers and non-smokers. Cotinine is therefore an appropriate b...

Claims

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Application Information

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IPC IPC(8): G01N30/06G01N30/02
Inventor 蔡继宝杨俊邓宇周平
Owner CHINA TOBACCO JIANGXI IND CO LTD
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