Chitosan hemostatic sponge made from maggot shells as well as preparation method and application thereof

A technology of chitosan and maggot shells, which is applied in medical science, absorbent pads, bandages, etc., can solve the problems of raw material quality control and production process reproducibility, and achieve the prevention of wound infection, selective antibacterial cell proliferation, and promotion of The effect of cell proliferation

Active Publication Date: 2011-02-16
FIELD OPERATION BLOOD TRANSFUSION INST OF PLA SCI ACAD OF MILITARY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the raw materials for the preparation of chitosan are mostly prepared from naturally collected shrimp and crab shells through high-concentration acid-base high-temperature extraction for a long time. There are great hidden dangers in the quality control of raw materials and the reproducibility of production processes.

Method used

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  • Chitosan hemostatic sponge made from maggot shells as well as preparation method and application thereof
  • Chitosan hemostatic sponge made from maggot shells as well as preparation method and application thereof
  • Chitosan hemostatic sponge made from maggot shells as well as preparation method and application thereof

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preparation example Construction

[0031] The preparation method of chitosan hemostatic sponge derived from fly maggot shells of the present invention may comprise the following steps:

[0032] 1) Crush the cleaned and dried artificially cultured fly maggot shells (through a 100-mesh screen), remove metal ions with 0.5-10mol / L hydrochloric acid solution, deproteinize with 1-20% sodium hydroxide solution, and use 1-20% Hydrogen peroxide solution decolorization, soaking in 30-60% sodium hydroxide solution for deacetylation (the number of deacetylation is 2-6 times, the reaction time is 4-18 hours, the preparation temperature is 40-90 ° C), dried into powder.

[0033] 2) The above-mentioned chitosan powder is dissolved in 0.5-10% dilute acetic acid solution at a concentration of 0.5-20% by weight and volume, poured into a mold for self-leveling and precooled at -20°C.

[0034] 3) Put the mold in step 2) into a freeze dryer to freeze-dry (-50°C, 48-72h), so that the material in the mold is a sponge with a micropor...

Embodiment 1

[0036] Embodiment 1, the preparation of fly maggot shell source chitosan hemostatic sponge

[0037] Prepare fly maggot shell source chitosan hemostatic sponge with method of the present invention, concrete method comprises the following steps:

[0038] 1) Crush the cleaned and dried artificially cultured fly maggot shells, remove metal ions with 0.5mol / L hydrochloric acid solution, deproteinize with 1% sodium hydroxide solution, decolorize with 1% hydrogen peroxide solution, and use 30% and 60% hydrogen peroxide solution respectively % sodium hydroxide solution for 2 gradient deacetylation, the single reaction time is 18 hours, the preparation temperature is 90°C, and it is dried into powder.

[0039] 2) The chitosan powder mentioned above is dissolved in 0.5% dilute acetic acid solution at a concentration of 0.5% by weight to volume, poured into a mold for self-leveling and precooled at -20°C.

[0040] 3) Put the mold into a freeze dryer and freeze-dry (-50°C, 72h), so that ...

Embodiment 2

[0042] Embodiment 2, the preparation of fly maggot shell source chitosan hemostatic sponge

[0043] Prepare fly maggot shell source chitosan hemostatic sponge with method of the present invention, concrete method comprises the following steps:

[0044] 1) Crush the cleaned and dried man-made fly maggot shells, remove metal ions with 5mol / L hydrochloric acid solution, deproteinize with 10% sodium hydroxide solution, decolorize with 10% hydrogen peroxide solution, use 30%, 40% , 50%, 60% sodium hydroxide solution for 4 gradient deacetylation, the single reaction time is 10 hours, the preparation temperature is 60°C, and it is dried into powder.

[0045] 2) Dissolving the above-mentioned chitosan powder in 5% dilute acetic acid solution at a concentration of 10% by weight to volume, pouring it into a mold for self-leveling and precooling.

[0046] 3) Put the mold into a freeze dryer to freeze-dry (-50°C, 48h), so that the material in the mold is a sponge with a microporous struc...

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Abstract

The invention discloses a chitosan hemostatic sponge made from maggot shells as well as a preparation method and application thereof. The chitosan hemostatic sponge is obtained by carrying out the following steps of: pulverizing cleaned and dried maggot shells; removing metal ions with a hydrochloric acid solution; removing protein with a sodium hydroxide solution; decoloring with a hydrogen peroxide solution; carrying out gradient deacetylation with the sodium hydroxide solution; drying to obtain powder; then dissolving the powder into a dilute acetic acid solution; freeze drying; and sterilizing. The product can effectively activate partial blood platelets, adsorb erythrocytes, promote the generation of partial thrombase, exert quick and stable hemostatic activity when the product is adhered to a partial tissue wound, have the effects of selective antibiosis and cell proliferation promotion and favorable effects of controlling moderate traumatic hemorrhage and tissue organ diffuse surface hemorrhage, can be suitable for external emergency treatment of traumatic hemorrhage, particularly suitable for implantation hemostasis in a surgery, and also has the functions of preventing wound infection of a wound surface and promoting healing of the wound surface.

Description

technical field [0001] The invention belongs to the field of medical hemostatic materials, and relates to a chitosan hemostatic sponge derived from fly maggot shells and a preparation method thereof, in particular to a hemostatic sponge capable of rapid hemostasis and various hemostatic mechanisms, which can be used for intraoperative implantation hemostasis and prevent wounds Wound infection, chitosan derived from fly maggot shell for promoting wound healing, its hemostatic sponge and its preparation method. Background technique [0002] Bleeding is the leading cause of death from all types of trauma. Rapid hemostasis is an important part of various operations and trauma first aid. Local hemostatic materials are the first choice for pre-hospital and intraoperative emergency hemostasis due to their simplicity and rapidity in the emergency treatment of surgery and sudden accidents. The research and development of ideal local hemostatic materials has always been a hot spot a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J9/28A61L15/28C08B37/08A61L15/64A61L15/42C08L5/08
Inventor 孟志云顾若兰吴卓娜朱晓霞窦桂芳孙文种甘慧王延琳
Owner FIELD OPERATION BLOOD TRANSFUSION INST OF PLA SCI ACAD OF MILITARY
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