Method for preparing resveratrol dimer
A technology of resveratrol and dimer, which is applied in the field of preparation of resveratrol dimer, can solve the problems of low specificity of oxidase, long reaction time, low conversion rate, etc., and achieve remarkable effect and synthetic The effect of shortening the time and improving the conversion efficiency
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Embodiment 1
[0022] Step 1. Enzymatic synthesis of resveratrol dimer
[0023] Dissolve 1 kg of resveratrol in 8 L of acetone solution with a mass concentration of 40%, add 3 kg of porous sintered glass rings with immobilized laccase, react at 35°C for 2 hours, and release the reaction solution after the reaction; Add the acetone solution dissolved in resveratrol to the reactor (the solid phase of immobilized laccase is left) to continue the reaction. The immobilized laccase can be used repeatedly (as the enzyme activity decreases, the reaction time can be prolonged), and the reaction time is collected. solution until the activity of immobilized laccase was reduced by half.
[0024] Step 2. Isolation of resveratrol dimer
[0025] The collected reaction solution is precipitated with an organic solvent chloroform (methanol or ethanol can also be used) to precipitate proteins, after filtration, the liquid phase is concentrated to a quarter of the original volume, then ethyl acetate is added f...
example 2
[0027] Step 1. Enzymatic synthesis of resveratrol dimer
[0028] Dissolve 1kg of resveratrol in 9L of acetone solution with a mass concentration of 50%, add 3.5kg of porous sintered glass ring with immobilized laccase, react at 40°C for 3 hours, and release the reaction solution after the reaction is completed; Add resveratrol-dissolved acetone solution to the reactor (leaving the solid phase of immobilized laccase) to continue the reaction. The immobilized laccase can be used repeatedly (as the enzyme activity decreases, the reaction time can be prolonged), and the collected The reaction solution was changed until the enzyme activity of the immobilized laccase was reduced by half.
[0029] Step 2. Isolation of resveratrol dimer
[0030] The collected reaction liquid is precipitated with the organic solvent methanol (chloroform or ethanol can also be used) to precipitate the protein, after centrifugation, the liquid phase is concentrated to a quarter of the original volume, t...
example 3
[0032] Step 1. Enzymatic synthesis of resveratrol dimer
[0033]Dissolve 1kg of resveratrol in 9L of ethyl acetate solution with a mass concentration of 30%, add 4kg of porous sintered glass ring with immobilized laccase, react at 45°C for 3 hours, and release the reaction solution after the reaction; In the reactor (the solid phase of immobilized laccase is left), add ethyl acetate solution dissolved with resveratrol to continue the reaction, and the immobilized laccase can be used repeatedly (as the enzyme activity decreases, the reaction time can be prolonged) , collect the reaction solution, and replace it until the enzyme activity of the immobilized laccase is reduced by half.
[0034] Step 2. Isolation of resveratrol dimer
[0035] The collected reaction solution was precipitated with neutral salt sodium sulfate (ammonium sulfate or zinc sulfate can also be used) to precipitate protein, after centrifugation, the liquid phase was concentrated to one-fifth of the original...
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