Folate-IgG conjugate as well as preparation method and application thereof

A technology of conjugates and folic acid, which is applied in the field of folic acid-IgG conjugates and its preparation and application, can solve the problems of no improvement in erythrocyte sedimentation rate and anemia, large side effects of treatment, difficult treatment, etc., and achieve fast blood clearance and reduce toxicity. Side effects, strong penetrating effects
CN101985471AActive Publication Date: 2011-03-16ZHEJIANG JIANFENG HANSHENG BIOSCI
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Applications(China)
Current Assignee / Owner
ZHEJIANG JIANFENG HANSHENG BIOSCI
Publication Date
2011-03-16

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Abstract

The invention provides a folate-IgG conjugate as well as a preparation method and application thereof. The folate-IgG conjugate is composed of folate and human souce IgG or recombinant IgG or the Fc section of IgG. The method for preparing the folate-IgG conjugate comprises the following steps: leading 0.1 to 30mol of the folate to react with 0.1 to 40mol of dicyclohexylcarbodiimide and 0.1 to 30mol of hydroxysuccinimide eater in dimethylsulfoxide (DMSO) at room temperature; centrifuging to extract precipitate; leading the precipitate to react with 0.01 to 2.0 times of the human source IgG or the recombinant IgG or the Fc section of the IgG at room temperature for 2 to 5 hours; and filtering the reactant through a molecular screen, and collecting the product, namely the folate-IgG conjugate. The folate-IgG conjugate can be used as a medicament for treating cancer and a medicament for treating autoimmune diseases. The folate-IgG conjugate can be specifically combined to cancer cells and macrophages activated in a patient body with the autoimmune diseases, but cannot be combined to normal cells, so that the problem of damaging the normal cells by the cancer treatment medicament and autoimmune disease medicament in the prior art can be solved.
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Description

technical field

[0001] The present invention relates to folic acid-IgG conjugate, its preparation method and its application. Background technique

[0002] Folic acid was extracted and purified from spinach by H.K. Mitchell in 1941. It is a water-soluble B vitamin composed of pteridine, p-aminobenzoic acid and glutamic acid residues. It is an essential substance for the human body. , has the effect of promoting the maturation of young cells in the bone marrow. If the lack of folic acid can lead to the increase of abnormal immature cells of red blood cells and the decrease of white blood cells. The latest research shows that folic acid may trigger the apoptosis of cancer cells, have a certain impact on the gene expression of cancer cells, and have a certain anti-cancer effect. The most important reason is that folic acid can bind to folic acid receptors in the body. Folate receptors (FR) are divided into Ξ± and Ξ² subtypes. Studies have shown that in 90% of ovarian cancer, adv...

Claims

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