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Method for efficiently separating Phytophthora capsici from aging disease tissue

A technology for Phytophthora capsici and pepper blight, applied in the direction of microorganism-based methods, biochemical equipment and methods, fungi, etc., can solve the problems of low separation efficiency, difficult separation and purification, relatively high requirements for disease samples, and achieve high separation efficiency , simple operation steps, the effect of eliminating pollution

Inactive Publication Date: 2012-08-08
INST OF PLANT PROTECTION FAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the methods for isolating Phytophthora capsici mainly include direct isolation of diseased tissue, trapping and isolation of bacteria-carrying soil or water samples, etc. Among them, the direct isolation of diseased tissue has relatively high requirements for the collected disease samples, and can only be obtained from fresh and obvious symptoms. Phytophthora capsici was isolated from the diseased samples; the trapping and isolation method of bacteria-carrying soil or water samples was easily affected by other bacteria in soil and water samples, and the isolation efficiency was low
Aging and decaying diseased tissues are seriously co-infected with other miscellaneous bacteria, and it is very difficult to isolate and purify Phytophthora capsici, and there are few studies on this aspect

Method used

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  • Method for efficiently separating Phytophthora capsici from aging disease tissue
  • Method for efficiently separating Phytophthora capsici from aging disease tissue
  • Method for efficiently separating Phytophthora capsici from aging disease tissue

Examples

Experimental program
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Effect test

Embodiment 1

[0025] The root samples collected from the vegetable base of Xindian Town, Fuzhou City, Fujian Province in the late stage of pepper blight were selected, and the isolation of Phytophthora capsici was carried out according to the following procedures:

[0026] (1) Take the aged root of pepper blight back to the laboratory, wash the soil and debris on the surface with tap water, scrape off the root skin with a utility knife, and cut it into tissue with a length of 2cm. If the diameter of the root exceeds 0.5cm, The xylem needs to be cut in half in the longitudinal direction on average. In a sterile ultra-clean bench, the cut tissue should be rinsed with sterile water for 3 times, soaked in 75% ethanol for 1.5 min, rinsed with sterile water for 3 times, and placed in an sterilized container. Blow dry the surface moisture in the ultra-clean bench;

[0027] (2) Rinse the prepared cucumber fruit under tap water to clean the surface debris, soak it in 75% ethanol for 0.5min, take it ...

Embodiment 2

[0033] The stem samples collected from the vegetable base of Xindian Town, Fuzhou City, Fujian Province in the late stage of pepper blight were selected, and the isolation of Phytophthora capsici was carried out according to the following procedures:

[0034] (1) Take the sample of the aged stem part of the pepper blight back to the laboratory, rinse off the surface debris with tap water, scrape off the skin with a utility knife, and cut it into stem tissue with a length of 2 cm. If the diameter of the stem part exceeds 0.5cm, it is necessary to split the xylem in half along the longitudinal direction. In a sterile ultra-clean bench, the stems are rinsed with sterile water for 3 times, soaked in 70% ethanol for 1.5min, rinsed with sterile water for 3 times, and placed in In the sterilized petri dish, dry the surface moisture in the ultra-clean workbench;

[0035] (2) Rinse the prepared cucumber fruit under tap water to clean the surface debris, soak in 70% ethanol for 1.0min, ...

Embodiment 3

[0041] The pepper fruits collected from the vegetable base of Nankou Town, Shaxian County, Fujian Province in the late stage of pepper blight were selected, and the isolation of Phytophthora capsici was carried out according to the following procedures:

[0042] (1) Take the aging diseased fruit samples of pepper blight back to the laboratory, rinse the surface debris with tap water, cut into pulp tissue with an area not larger than 2×2cm, and rinse the pulp tissue with sterile water in a sterile ultra-clean bench 3 times, soak in 80% ethanol for 1 min, rinse with sterile water 3 times, place in a sterilized petri dish, and dry the surface moisture in an ultra-clean workbench;

[0043] (2) Rinse the prepared cucumber fruit under tap water to clean the surface debris, soak it in 75% ethanol for 0.5min, take it out, and dry the remaining ethanol on the surface on the flame of a wine lamp, and use a sterile utility blade to clean it An opening of an appropriate size is cut at the...

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Abstract

The invention relates to a method for efficiently separating and purifying bacterial strains of Phytophthora capsici from a diseased tissue of aging Phytophthora capsici Leonian, the method includes steps of acquisition of the diseased tissue of Phytophthora capsici Leonian, surface sterilization, preparation of Cucumis sativus, surface sterilization of the Cucumis sativus, culturing the diseasedtissue and the Cucumis sativus, making a selective culture medium, and separation, purification and storage of the Phytophthora capsici. The method ensures separating Phytophthora capsici from the aging disease tissue, and the method is simple, easy, efficient, free from Miscellaneous bacteria pollution and adapted to separation of mass bacterial strains.

Description

technical field [0001] The invention relates to the technical field of separation of plant pathogenic bacteria, in particular to an efficient separation method of Phytophthora capsici in aging diseased tissues. Background technique [0002] Pepper is a traditional industry in my country. Since the reform and opening up, with the advancement of market economy, my country's pepper industry has developed at a rate of 7% per year, and my country's pepper exports are also growing. South Korea, Japan, Australia, the United States and Southeast Asia have become my country's A perennial importer of peppers. Due to the strong adaptability and wide cultivation area of ​​pepper, the economic and social benefits of planting pepper are very high, which is of great significance to the annual supply of Chinese vegetable market. Pepper has become a good helper for some poor mountainous areas in my country to get rid of poverty and become a good helper for urban vegetable baskets. Home cook...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12R1/645
Inventor 兰成忠陈庆河李本金李炜吴敏亮翁启勇
Owner INST OF PLANT PROTECTION FAAS
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