Ocytocic for inducing stichopus japonicus to lay eggs in vivo

Abstract

The invention discloses an ocytocic for inducing stichopus japonicus to lay eggs in vivo, which is prepared by the following steps of: dissolving NGLWY amide in ultrapure water to obtain 1mu mol / L mother liquid, pouring the mother liquid into 0.5mol / L seawater solution of KCl to obtain the ocytocic of which the NGLWY amide concentration is 1nmoll / L. The NGLWY amide and KCl, which are rationally blended and interacted, directly act on the gonad of the stichopus japonicus to induce the oocytes of the stichopus japonicus to be mature and remove follicle, so that all individuals are basically ovulate at one time of parturition, and the parturition ratio is as high as over 90 percent. The used amount of the ocytocic can guarantee that the parent stichopus japonicus does not produce undesired stuff, that the ovulation rate is greatly improved, that average egg laying amount is increased to 4 million which accounts for over 70 percent of the total brood amount, so that the use efficiency of the parent stichopus japonicus is greatly improved, the problem that the parent stichopus japonicus produces the undesired stuff caused by a large amount of KCl injected simply is solved, and the problem of small egg laying amount caused by simply using the NGLWY amid is solved.

Description

Technical field: [0001] The invention relates to an oxytocic agent for inducing oviposition of sea cucumbers in vivo, in particular to an in vivo induction agent capable of synchronous oviposition of sea cucumbers with large ovum discharge and good quality, which can greatly improve oxytocin efficiency, fertilization rate and hatching rate An oxytocin for sea cucumber spawning. Background technique: [0002] Sea cucumber has long been famous as a delicacy on the table of people in my country, Southeast Asia and other countries along the Pacific Ocean and the West Indian Ocean. Especially Chinese people in the world have always had the habit of eating sea cucumber as a "food tonic". Nowadays, sea cucumber has become more and more popular because of its rich nutritional value. more popular with people. The increase in demand has led to an increase in harvesting intensity, resulting in a sharp decline in the natural growth of sea cucumber resources and production in my country,...

AI Technical Summary

Problems solved by technology

Although the literature points out that the injection of NGLWY amide in vivo can induce the release of sperm and eggs of sea cucumber and discloses that the injection volume of each individual is 0.1% (v / w), and the optimal concentration is 1 nmol / L, but experiments have shown that: NGLWY alone can be used as an inducer of labor. For example, the average weight of mature ginseng with an average weight of 210 grams is only 430,000, the survival rate of planktonic larvae is only 20-50%, and the metamorphosis rate of immature ginseng is only 15-30%.