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Method for separating and purifying ellagitannic acid in raspberry

A technology for the separation and purification of ellagitannin, which is applied in the field of separation and purification of ellagitannin in Rubus, can solve the problems that the product content is greatly affected, the separation and purification cannot be achieved, and there are no reports, so as to improve the drug Good effect, economic and social benefits, and high separation purity

Active Publication Date: 2012-07-25
NANNING GODSEND BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although Rubus ellagitannic acid has been applied in Japan and other countries, industrial separation and purification methods have not been reported. Even in Japan, the products used so far have an ellagitannic acid content of only about 5%, and It is mostly used together with ragweed, the reason is that rubus and ellagitannin have excellent water solubility and alcohol solubility, and it is difficult to separate the two, while rubus has a very high content in Rubus, which has a great influence on the content of the product Large, if there is no advanced technology, it is impossible to achieve the purpose of separation and purification

Method used

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  • Method for separating and purifying ellagitannic acid in raspberry
  • Method for separating and purifying ellagitannic acid in raspberry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] In the stainless steel reaction kettle, add the dried leaves of the former drug Rubus in parts by weight as 50 parts, water as 600 parts and protective agent sodium pyrosulfite as 6 parts, boil and keep warm for 1 hour, filter, and the extract is for subsequent use; Continue to add 600 parts of water and 6 parts of protective agent in parts by weight to the slag, boil and keep warm for 1 hour, filter, and combine the secondary extract; pass the cooled extract through a DM101 macroporous adsorption resin column, and wash with water When it is clear, the mother liquor and washing liquid are combined for subsequent use; the mother liquor and washing liquid are ultrafiltered through an organic membrane with a molecular weight of 30,000 Daltons used in the first pass, and then the organic membrane molecular weight used is 1,000 Daltons for the second organic filtration. Membrane ultrafiltration is concentrated, and the retentate is concentrated and dried to obtain a crude pro...

Embodiment 2

[0029] Adding the whole plant of the former drug Rubus in parts by weight in the stainless steel retort is 55 parts, water is 650 parts and protective agent sodium pyrosulfite is 7 parts, boils and keeps warm for 1.5 hours, filters, and the extract is for subsequent use; Continue to add 650 parts of water and 7 parts of protective agent to the slag, boil and keep warm at 0.5, filter, and combine the secondary extract; pass the cooled extract through DM101 macroporous adsorption resin column or similar type packing The column is washed with water until it is clear, and the mother liquor and washing solution are combined for subsequent use; the mother liquor and washing solution are passed through the first ultrafiltration with an organic membrane molecular weight of 20,000 Daltons, and then the organic membrane molecular weight used is 200 Daltons. The second organic membrane ultrafiltration is concentrated, and the retentate is concentrated and dried to obtain a crude product o...

Embodiment 3

[0032] In the enamel reaction tank, adding the dried leaves of the former drug Rubus in parts by weight is 45 parts, water is 550 parts and protective agent sodium pyrosulfite is 5 parts, boiling and insulating is 1 hour, filters, and the extract is for subsequent use; Continue to add 550 parts of water and 5 parts of protective agent to the slag, boil and keep warm for 1.5 hours, filter, and combine the secondary extract; pass the cooled extract through a DM101 macroporous adsorption resin column or similar models The packed column is washed with water until it is clear, and the mother liquor and water washing liquid are combined for later use; the mother liquor and water washing liquid are passed through the ultrafiltration of an organic membrane with a molecular weight of 26,000 Daltons used in the first pass, and an organic membrane with a molecular weight of 1,000 Daltons is used again. Carry out the organic membrane ultrafiltration concentration for the second time, obtai...

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Abstract

The invention discloses a method for separating and purifying ellagitannic acid in raspberry. In the method, a raspberry extract is obtained by an aqueous extraction method, the ellagitannic acid of the raspberry is prepared by a method of combining macroporous adsorbent resin separation technology, ultrafiltration membrane separation technology and supercritical CO2 extraction technology, and the separation purity of the ellagitannic acid is improved by over 95 percent from about 5 percent of the conventional process technology; and a by-product, namely rubusoside of which the total glycoside content is more than or equal to 70 percent is prepared, so that medicinal ingredients, which are different to separate, in the ellagitannic acid and the rubusoside which have high water solubility and alcohol solubility are separated effectively, and the pharmaceutical effect of the raspberry is improved. The method is simple, has high separation purity, is easy to operate, is suitable for producing the ellagitannic acid and the rubusoside industrially, and has good economic and social benefits.

Description

Technical field: [0001] The invention belongs to the technical field of biochemistry, and relates to a method for separating and purifying ellagitannin in Rubus. Background technique: [0002] Rubus Suavissimus S.Lee, commonly known as sweet tea, is a perennial plant of the Rosaceae Rubus genus. It is a unique plant in the Dayao Mountains of Guangxi. The whole plant is used as medicine to nourish the liver and stomach, dispel wind and relieve pain. It is widely used among the people because of its high sweetness in its leaves, and it is cool in nature and non-toxic. Modern pharmacology has proved that the polyphenolic substance ellagitannin in Rubus has anti-allergic effects, is safe and non-toxic, so Rubus has become the most effective natural anti-pollen allergy product in the Japanese market. [0003] Although Rubus ellagitannic acid has been applied in Japan and other countries, industrial separation and purification methods have not been reported. Even in Japan, the pr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H13/08C07H1/08A23L1/221A23L27/10
CPCY02P20/54
Inventor 阮俊李岳陈跃新廖正福
Owner NANNING GODSEND BIOTECH
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