Chip kit for quantitative detection of veterinary drug residue
A technology for quantitative detection and veterinary drug residues, applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of complex operation, low specificity, and high false positives, and achieve the effect of accurate detection and high-sensitivity quantitative detection.
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[0018] The present invention will be described in further detail below in conjunction with accompanying drawing embodiment:
[0019] 1. Extraction of residual veterinary drug in the sample: Mince the tissue sample, add veterinary drug residual extraction reagent, homogenate, centrifuge, take the supernatant, and concentrate.
[0020] 2. Hybridization: add the extract to the chip, and perform a competitive immunoreaction for 1 hour to combine the veterinary drug with the specific monoclonal antibody; wash with distilled water for 5 minutes, and dry; add the phosphorescently labeled secondary antibody and react for 1 hour.
[0021] 3. Cleaning: The hybridized chip was washed with 42°C preheated washing solution for 4 minutes, then washed once with 42°C preheated distilled water, and centrifuged at 1500rpm.
[0022] 4. Chip scanning and result interpretation: The cleaned chip is scanned and analyzed using the UPT biosensor under the chip analysis system for quantitative detection...
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