Chip kit for quantitative detection of veterinary drug residue

A technology for quantitative detection and veterinary drug residues, applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of complex operation, low specificity, and high false positives, and achieve the effect of accurate detection and high-sensitivity quantitative detection.

Inactive Publication Date: 2011-04-13
NINGBO BOAO BIOENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The technical effect of this patented method is that it allows veterinarians to easily test different types of drugs on their own skin without having them wait longer than usual or even worse by doing things like swabbling up blood from animals' veins instead of waiting long periods before testing any new medication. This makes analysis faster but more accurately compared to previous methods such as liquid chromatography-mass spectrometry (LCMS).

Problems solved by technology

This patented technical problem addressed by this patents relates to detecting harmful chemical compounds present during production processes for animals that can cause negative side-effects like medication resistant germs (microorganisms) and other contamination issues. Traditional testing techniques involve lengthy procedures and often result in poor accuracy due to factors like preservatives being left behind from previous uses overtime.

Method used

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  • Chip kit for quantitative detection of veterinary drug residue
  • Chip kit for quantitative detection of veterinary drug residue

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Experimental program
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Embodiment Construction

[0018] The present invention will be described in further detail below in conjunction with accompanying drawing embodiment:

[0019] 1. Extraction of residual veterinary drug in the sample: Mince the tissue sample, add veterinary drug residual extraction reagent, homogenate, centrifuge, take the supernatant, and concentrate.

[0020] 2. Hybridization: add the extract to the chip, and perform a competitive immunoreaction for 1 hour to combine the veterinary drug with the specific monoclonal antibody; wash with distilled water for 5 minutes, and dry; add the phosphorescently labeled secondary antibody and react for 1 hour.

[0021] 3. Cleaning: The hybridized chip was washed with 42°C preheated washing solution for 4 minutes, then washed once with 42°C preheated distilled water, and centrifuged at 1500rpm.

[0022] 4. Chip scanning and result interpretation: The cleaned chip is scanned and analyzed using the UPT biosensor under the chip analysis system for quantitative detection...

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Abstract

The invention relates to a chip kit for the quantitative detection of veterinary drug residues, comprising a veterinary drug extraction reagent, a chip and a molecular hybridization reagent. The chip kit is characterized in that the chip is fixed with a specific antibody respectively aiming at clenobuterol hydrochloride, ractopamine, streptomycin, chloramphenicol, ciprofloxacin, nitrofuran, sulfametoxydiazine and tetracycline. The invention can simultaneously quantificationally detect eight veterinary drug residues contained in four samples, only needs four hours in the detecting process, automatically interprets a result through a system, provides an ideal mean for the fast quantitative detection of the veterinary drug residues contained in meats, poultry and dairy products and has good application prospect.

Description

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Claims

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Application Information

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Owner NINGBO BOAO BIOENG
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