Salt-resistant degrading bacterium for treating high-concentration persistent organic wastewater and application thereof
An organic sewage, high-concentration technology, applied in the direction of biological water/sewage treatment, water/sludge/sewage treatment, microbial-based methods, etc., can solve the problem of low salinity tolerance and high mineralization of phenol-reducing bacteria High-salinity, high-salinity sewage and other problems, to achieve good biological stability, good microbial growth, and stable system effluent
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Embodiment 1
[0045] Example 1. Isolation, identification and preservation of the salt-tolerant bacteria JF-2, JF-3 and JF-6 of the present invention
[0046] 1. Artificial screening and domestication of microorganisms
[0047] Take 250mL of high-concentration chemical sewage water samples from petrochemical plants (collected on December 12, 2008, crude oil contaminated water samples from Suizhong SZ-361 Crude Oil Treatment Plant) as the original bacterial source, and use the conventional batch domestication method for manual screening and acclimatization, the specific operations include: take 0.2mL of the original bacterial source and smear it on the agar plate medium with 100mg / L phenol as the only carbon source, do three parallels for each original bacterial source, 30 ℃, 48h culture (taken at the same time Spread 0.2mL of the original bacterial source on the complete nutrient agar plate medium, make three parallel samples for each, culture at 30°C for 48 hours, as a control); and transf...
Embodiment 2
[0061] Example 2, Experiments of salt-tolerant bacteria JF-2, JF-3, and JF-6 degrading organic matter in sewage under different conditions
[0062] Seed bacterium liquid: the bacteria are placed in the enrichment medium (prepared with reference to the prior art, the basic composition includes: phenol 1g / L, (NH 4 ) 2 SO 4 1.5g / L, KH 2 PO 4 0.5g / L, K 2 HPO 4 0.5g / L, NaCl 1.0g / L, MnSO 4 ·H 2 O trace, FeSO 4 ·7H 2 O trace, etc.) cultivated to a concentration of 10 8 -10 9 cells / mL, as the seed solution, the experimental research of this embodiment and the pilot test research of the subsequent embodiments are carried out.
[0063] Simulated sewage formula: KH 2 PO 4 0.5g, K 2 HPO 4 0.5g, MgSO 4 ·7H 2 O 0.2g, CaCl 2 0.1g, NaCl 0.2g, MnSO 4 ·H 2 O trace, FeSO 4 ·7H 2 O trace, NH 4 NO 3 1.0g, 1L of distilled water, according to the needs of the experiment, add some phenolic pollutants. The phenolic pollutants: a mixture of phenol, p-tolualdehyde, dibutyl...
Embodiment 3
[0077] Embodiment 3, the pilot test of using salt-tolerant bacteria JF-2, JF-3, JF-6 to degrade organic matter in sewage
[0078] In this example, the salt-tolerant bacteria JF-2, JF-3, and JF-6 provided by the present invention are used to treat high-concentration chemical wastewater, and the specific method is as follows:
[0079] (1) Acidify the high-concentration chemical wastewater in the acidification pool 1, and control the pH value of 6 to 10, preferably about 8. After the acidification, an organic phase separated from the water phase is produced, which can be recycled as a resource product; ( 2) In order to meet the basic requirements of microorganisms for salt content, the acidified chemical sewage is diluted (the dilution factor is about 10), and nutrients are added at the same time; In the reactor 3, the reactor controls the temperature in the bioreactor through the heating device and the temperature control instrument 5 to keep it at about 30°C, adopts the hydroly...
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