Bacillus spore and trichoderma chlamydospore compound preparation and preparation method and application thereof

A technology of compound preparation and chlamydospore, which is applied in the field of agricultural biology and biology, can solve the problems that the stability of the control effect is not effectively guaranteed, and the survival rate of Trichoderma is difficult to meet the requirements, so as to improve the utilization rate of fertilizers and strengthen the vitality and stress resistance, the effect of promoting plant growth

Active Publication Date: 2011-04-27
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Generally, for preparations with conidia as the active ingredient, the time to ensure the content of viable bacteria will not exceed 3 months. The survival amount of Trichoderma in the preparations received in the later stage is difficult t

Method used

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  • Bacillus spore and trichoderma chlamydospore compound preparation and preparation method and application thereof
  • Bacillus spore and trichoderma chlamydospore compound preparation and preparation method and application thereof
  • Bacillus spore and trichoderma chlamydospore compound preparation and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0042] Embodiment 1: 500L tank liquid fermentation of Trichoderma T4 produces chlamydospore research

[0043] This example studies the chlamydospore production of Trichoderma T4 in a 500L fermenter. The strain used is Trichoderma viride (T. viride) T4, which was isolated from plant rhizosphere soil and can be purchased from the State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology. Bioengineering, "Study on solid fermentation process of Trichoderma viride T4 and its preparation stability", 2008, Vol. 25, No. 12, pp. 52-56).

[0044] In the fermentation medium, the temperature is 28-30°C, the seed inoculum is 6-9%, and the seed concentration is 10%. 5 -10 6 liquid fermentation of Trichoderma is carried out for 96 hours under a tank pressure of 0.03-0.05MPa, an aeration ratio of 1:2 to 2:1, and a fermentation broth rich in Trichoderma chlamydospores is obtained. The composition of the fermentation medium used was: bran 0.5%, (NH 4 ) ...

Example Embodiment

[0046] Example 2: Influence of vegetable oil on chlamydospore of Trichoderma T4 bacteria

[0047] The fermentation process of Example 1 was repeated except that four concentrations of soybean oil were added to the medium, and then the chlamydospore yield of Trichoderma T4 was determined.

[0048] The results showed that compared with not adding soybean oil, adding soybean oil could significantly promote the production of chlamydospores of Trichoderma T4. With the increase of soybean oil concentration, the chlamydospores produced gradually increased. The chlamydospore has the largest number, reaching 3.3×10 7 spores / mL, the amount of chlamydospores produced at the concentration of 2g / L also reached 3.28×10 7 pcs / mL, almost close to the yield of 5g / L. At the same time, at a concentration of 5g / L, it was found that there were still large oil droplets in the fermentation broth during microscopic examination and counting, indicating that the concentration of 5g / L was excessive an...

Example Embodiment

[0049] Example 3: Preparation of Trichoderma WP

[0050] As described in Example 1, a 500L fermentor was used to obtain a chlamydospore fermentation broth, conditions: 30°C, 180 r / min, 2.1 L / min, an inoculum size of 6%, and after 96 hours of chlamydospore formation, fermentation Finish. After high-speed homogenization of the fermentation broth (5000r / min for 3 minutes), add 10% (W / W) carrier and 1% (W / W) (based on the total weight of the fermentation broth) wetting and dispersing agent, spray with Drying (conditions: inlet temperature 155°C, outlet temperature 105-110°C, feeding rate 4.5L / h) and drying to make wettable powder. It is tested according to the wettable powder standard. Table 1 lists different carriers (light calcium carbonate (1250 mesh), kaolin (1250 mesh), silica (500 mesh), talc (800 mesh), diatomaceous earth (500 mesh), bentonite (325 mesh) Item)) on the viability of Trichoderma.

[0051] Table 1

[0052]

[0053] It can be seen from Table 1 that after...

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Abstract

The invention provides a compound preparation for controlling plant diseases. The compound preparation consists of a bacillus spore and a trichoderma chlamydospore. The invention also provides a method for preparing the compound preparation and application of the compound preparation.

Description

technical field [0001] This application relates to the field of biotechnology, especially the field of agricultural biotechnology. Specifically, the application relates to a composite preparation of Bacillus spores and Trichoderma chlamydospores, its preparation method and application. Background technique [0002] Bacillus subtilis (Bacillus subtilis) is a mesophilic aerobic spore-forming rod-shaped bacterium with rich and diverse physiological characteristics, widely distributed, and easy to isolate and culture. The bacterium exists widely in nature, is non-toxic and harmless to humans and animals, does not pollute the environment, can produce a variety of antibiotics and enzymes, has broad-spectrum antibacterial activity and strong resistance to stress. Bacillus subtilis can not only widely exist in the external environment such as soil, plant rhizosphere, and body surface, but also is a common endophytic bacterium in plants, especially in the roots and stems of plants. ...

Claims

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Application Information

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IPC IPC(8): A01N63/02A01N25/14A01P3/00A01P21/00
Inventor 王伟王剑夏斯琴李小礼
Owner EAST CHINA UNIV OF SCI & TECH
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