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Bacillus spore and trichoderma chlamydospore compound preparation and preparation method and application thereof

A technology of compound preparation and chlamydospore, which is applied in the field of agricultural biology and biology, can solve the problems that the stability of the control effect is not effectively guaranteed, and the survival rate of Trichoderma is difficult to meet the requirements, so as to improve the utilization rate of fertilizers and strengthen the vitality and stress resistance, the effect of promoting plant growth

Active Publication Date: 2011-04-27
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Generally, for preparations with conidia as the active ingredient, the time to ensure the content of viable bacteria will not exceed 3 months. The survival amount of Trichoderma in the preparations received in the later stage is difficult to meet the requirements, and the stability of its control effect has not been effectively guaranteed. In the prevention and control of plant diseases, other control measures such as chemical control cannot be completely replaced at present.

Method used

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  • Bacillus spore and trichoderma chlamydospore compound preparation and preparation method and application thereof
  • Bacillus spore and trichoderma chlamydospore compound preparation and preparation method and application thereof
  • Bacillus spore and trichoderma chlamydospore compound preparation and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1: the research of the 500L tank liquid fermentation of Trichoderma T4 producing chlamydospores

[0043] In this example, the production of chlamydospores of Trichoderma T4 in a 500L fermenter was studied. The bacterial strain used is Trichoderma viride (T.viride) T4, which is isolated from plant rhizosphere soil and can be purchased from the State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology. Bioengineering, "Studies on Solid Fermentation Process of Trichoderma viride T4 and Its Preparation Stability", 2008, Vol.25, No.12, pp. 52-56).

[0044] In the fermentation medium, the temperature is 28-30°C, the seed inoculation amount is 6-9%, and the seed concentration is 10 5 -10 6 Individuals / mL, aeration ratio of 1:2 to 2:1, and tank pressure of 0.03-0.05 MPa, carry out liquid fermentation on Trichoderma for 96 hours, thereby obtaining a fermentation liquid rich in Trichoderma chlamydospores. The composition of the ...

Embodiment 2

[0046] Embodiment 2: Vegetable oil is to the impact of Trichoderma T4 bacterial chlamydospore

[0047] The fermentation process of Example 1 was repeated, except that four concentrations of soybean oil were added to the medium, and then the chlamydospore yield of Trichoderma T4 was determined.

[0048] It was found that, compared with no soybean oil, the addition of soybean oil could significantly promote the production of Trichoderma T4 chlamydospores. With the increase of soybean oil concentration, the number of chlamydospores produced gradually increased. The number of chlamydospores was the largest, reaching 3.3×10 7 Individual / mL, the amount of chlamydospores produced under the concentration of 2g / L also reached 3.28×10 7 / mL, almost close to the yield of 5g / L. Find under the concentration of 5g / L simultaneously, find that there is still big oil droplet to exist in the fermented liquid during microscopic examination counting, illustrate that the concentration of 5g / L is...

Embodiment 3

[0049] Embodiment 3: the preparation of Trichoderma wettable powder

[0050] According to the description in Example 1, use a 500L fermenter to cultivate and obtain a chlamydospore fermentation broth, conditions: 30°C, 180r / min, 2.1L / min, the inoculum size is 6%, after 96 hours a large amount of chlamydospores are formed, ferment Finish. After the fermentation broth is homogenized at high speed (5000r / min homogenization for 3 minutes), add 10% (W / W) carrier and 1% (W / W) (based on the total weight of fermentation broth) wetting and dispersing agent, spray Dry (conditions: inlet temperature 155°C, outlet temperature 105-110°C, feed rate 4.5L / h) and make wettable powder after drying. It is tested according to wettable powder standards. Table 1 lists different carriers (light calcium carbonate (1250 mesh), kaolin (1250 mesh), white carbon black (500 mesh), talcum powder (800 mesh), diatomaceous earth (500 mesh), bentonite (325 order)) on the viability of Trichoderma.

[0051] ...

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Abstract

The invention provides a compound preparation for controlling plant diseases. The compound preparation consists of a bacillus spore and a trichoderma chlamydospore. The invention also provides a method for preparing the compound preparation and application of the compound preparation.

Description

technical field [0001] This application relates to the field of biotechnology, especially the field of agricultural biotechnology. Specifically, the application relates to a composite preparation of Bacillus spores and Trichoderma chlamydospores, its preparation method and application. Background technique [0002] Bacillus subtilis (Bacillus subtilis) is a mesophilic aerobic spore-forming rod-shaped bacterium with rich and diverse physiological characteristics, widely distributed, and easy to isolate and culture. The bacterium exists widely in nature, is non-toxic and harmless to humans and animals, does not pollute the environment, can produce a variety of antibiotics and enzymes, has broad-spectrum antibacterial activity and strong resistance to stress. Bacillus subtilis can not only widely exist in the external environment such as soil, plant rhizosphere, and body surface, but also is a common endophytic bacterium in plants, especially in the roots and stems of plants. ...

Claims

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Application Information

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IPC IPC(8): A01N63/02A01N25/14A01P3/00A01P21/00
Inventor 王伟王剑夏斯琴李小礼
Owner EAST CHINA UNIV OF SCI & TECH
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