Taxus mairei immunological adjuvant and influenza vaccine containing same

An immune adjuvant and influenza vaccine technology, which is applied to medical preparations containing active ingredients, antiviral agents, antibody medical components, etc., can solve problems such as limited vaccine production and supply, unavoidable side effects, and slow vaccine onset time , to achieve the effects of weakened activity, obvious injection local swelling, and small side effects

Active Publication Date: 2011-04-27
INST OF MEDICAL BIOLOGY CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] In order to solve the current problems of single immune adjuvant, unavoidable side effects, limited supply of vaccine production, difficulty in coping with the large demand for vaccines when infectious disea

Method used

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  • Taxus mairei immunological adjuvant and influenza vaccine containing same
  • Taxus mairei immunological adjuvant and influenza vaccine containing same
  • Taxus mairei immunological adjuvant and influenza vaccine containing same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Preparation of the ethanol extract of Taxus Mandia: Take 50g of dried Taxus Mandia branches and leaves, grind them into powder, put them in a container, use 4 times 200 ml of ethanol with a mass concentration of 70%, and conduct heating and reflux extraction as usual , where the heating temperature is 50°C each time, the reflux time is 60 minutes, and the number of extractions is 2 times. After filtration, the filtrate is decompressed to recover ethanol, and the remaining filtrate is concentrated and dried to obtain an extract.

Embodiment 2

[0048] Preparation of the water extract of Taxus Mandia: Take 50g of dried Taxus Mandia branches and leaves, pulverize them into powder, put them in a container, heat and decoct them with 8 times of 400 ml distilled water as usual, and decoct each time The cooking time is 4 hours, the number of extractions is 3 times, filtered, and after combining the filtrates, the filtrates are concentrated and dried to obtain extracts.

Embodiment 3

[0050] Preparation of nanoemulsion vehicle:

[0051] 1) Prepare materials according to the following mass ratio: 0.475g water for injection, 1g isopropyl myristate (IPM), 1g 1,2-propanediol, 1g Tween80;

[0052] 2) Add 1g Tween80 and 1g 1,2-propanediol into the mixing bottle, and keep stirring for 1 to 10 minutes at a speed of 200 rpm at room temperature. After mixing, add 1g of isopropyl myristate and mix a homogeneous mixture;

[0053] 3) Add 0.475 g of water for injection dropwise to the mixture of the above 2), and at the same time, stir the mixture at a rotation speed of 200 rpm at room temperature until the liquid is transparent to obtain a nanoemulsion;

[0054] 4) The nanoemulsion prepared in 3) was tested as follows: 1. The particle size range of 19.205-24.896nm was tested by the Malvern particle size detector, and it was qualified; 2. After centrifugation at 3000rpm and 15min, the nanoemulsion system was not separated. 3. No change in the nanoemulsion system after ...

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Abstract

The invention provides a taxus mairei immunological adjuvant and an influenza vaccine containing the same. The taxus mairei immunological adjuvant is characterized by consisting of the following components in percentage by mass: 0.001 to 100 percent of taxus madia extract, and 0 to 99.999 percent of vector. The immunological adjuvant can better improve the immunogenicity of the vaccine, and greatly reduces the consumption of vaccine antigens; the consumption of the vaccine antigens used by using the taxus mairei extract as the adjuvant is half of the consumption of vaccine antigens used by only using aluminum hydroxide as the adjuvant or less, and ideal immunological protective effect can be achieved likewise; the immunological adjuvant has high safety and low side effect; and a method for preparing the influenza vaccine by using the taxus mairei extract as the adjuvant is simple and convenient, and the quality of the vaccine is easy to control.

Description

technical field [0001] The invention relates to a yew immune adjuvant and an influenza vaccine containing the yew immune adjuvant, which belongs to the field of biotechnology. technical background [0002] Mandia yew (Taxus madia) is a natural hybrid variety of yew. Its female parent is Northeast yew (T.cuspidata) and its male parent is European yew (T.bauata). It has grown in the United States and Canada. It has a history of nearly 100 years. my country introduced from Canada in the mid-1990s, carried out artificial propagation, and cultivated in Sichuan, Guangxi, Shandong and Yunnan. [0003] Taxus mairei is a relict plant from the Quaternary Ice Age. It is a rare and endangered species in the world. There are eleven species in the world. At present, there are four species and one variety in my country, namely, Yunnan yew, Tibetan yew, and Northeast yew. , Chinese yew and southern yew (variety). [0004] Mandia yew is an evergreen shrub cultivated artificially. It ha...

Claims

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Application Information

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IPC IPC(8): A61K39/39A61K39/145A61P31/16
Inventor 李映波陈芹芹李慧高天荣
Owner INST OF MEDICAL BIOLOGY CHINESE ACAD OF MEDICAL SCI
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