Method for inhibiting growth of cyanobacteria
A technology of cyanobacteria and algal liquid, applied in the field of eutrophic lake treatment and inhibition of cyanobacteria growth, can solve problems such as lack of practical operability, and achieve the effects of no secondary pollution, strong operability, and growth inhibition.
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Embodiment 1
[0042] In this test, a blank tank (non-ventilated tank) and an acidification tank (ventilated tank) are set.
[0043] 1. Algae cultivation: put 1L1 Microcystis aeruginosa species in the logarithmic growth phase into a water tank (40mm×25mm×30mm in size) filled with 10L of M11 culture solution for cultivation, and the concentration of the algae solution is about 10 6 cell / L. The culture conditions are: light 2000lx, temperature 27°C, light-dark ratio 14:10.
[0044] 2. Acidification of the culture solution: acidify the solution on the second day of cultivation, and set the pH to 6.0.
[0045] 3. Continuous ventilation: the frequency of ventilation is 2 times / day, at 10:00 and 16:00 every day. Each aeration was stopped when the pH of the solution reached 6.0.
[0046] 4. Monitor the pH and chlorophyll a of the culture solution: measure the pH and chlorophyll a of the solution before and after daily aeration. The results showed that the pH value of the algae liquid in the aci...
Embodiment 2
[0049] In this test, one blank tank (non-ventilated tank) and three acidizing tanks (ventilated tank) are set.
[0050] 1. Cultivation of algae species: insert 1L of Microcystis aeruginosa species in the logarithmic growth phase into 10L of M11 culture solution (the formula of M11 culture solution is such that 1L of deionized water contains NaNO 3 100mg, K 2 HPO 4 10mg, MgSO 4 ·7H 2 O75mg, CaCl 2 2H 2 O40mg, Na 2 CO 3 20mg, Ferric Citrate 6mg, Na 2 EDTA·2H 2 O1 mg. In this experiment, high-purity water was used instead of deionized water to eliminate the interference of bacteria to the greatest extent) in a tank (size: 40mm×25mm×30mm), the concentration of algae liquid was about 10 6 cell / L. The culture conditions are: light 2000lx, temperature 27°C, light-dark ratio 14:10.
[0051] 2. Acidification of the culture solution: On the second day of the culture, the solutions in the three tanks were acidified, and the pHs were set to 5.5, 6.0 and 6.5 respectively.
...
Embodiment 3
[0056] In this test, one blank tank (non-ventilated tank) and three acidizing tanks (ventilated tank) are set.
[0057] 1. Cultivation of algae species: insert 1L of Microcystis aeruginosa species in the logarithmic growth phase into 10L of M11 culture solution (the formula of M11 culture solution is such that 1L of deionized water contains NaNO 3 100mg, K 2 HPO 4 10mg, MgSO 4 ·7H 2 O75mg, CaCl 2 2H 2 O40mg, Na 2 CO 3 20mg, Ferric Citrate 6mg, Na 2 EDTA·2H 2 O1 mg. In this experiment, high-purity water was used instead of deionized water to eliminate the interference of bacteria to the greatest extent) in a tank (size: 40mm×25mm×30mm), the concentration of algae liquid was about 10 6 cell / L. The culture conditions are: light 2000lx, temperature 27°C, light-dark ratio 14:10.
[0058] 2. Acidification of the culture solution: On the 15th day of the culture (the time when it entered the stable phase), the solutions in the three tanks were acidified, and the pHs wer...
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