Method for inhibiting growth of cyanobacteria

A technology of cyanobacteria and algal liquid, applied in the field of eutrophic lake treatment and inhibition of cyanobacteria growth, can solve problems such as lack of practical operability, and achieve the effects of no secondary pollution, strong operability, and growth inhibition.

Inactive Publication Date: 2011-05-04
CHINA UNIV OF GEOSCIENCES (BEIJING)
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This is not practical for treating actual polluted lakes by using the principle of changing the pH as a condition to inhibit the growth of algae

Method used

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  • Method for inhibiting growth of cyanobacteria
  • Method for inhibiting growth of cyanobacteria
  • Method for inhibiting growth of cyanobacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] In this test, a blank tank (non-ventilated tank) and an acidification tank (ventilated tank) are set.

[0043] 1. Algae cultivation: put 1L1 Microcystis aeruginosa species in the logarithmic growth phase into a water tank (40mm×25mm×30mm in size) filled with 10L of M11 culture solution for cultivation, and the concentration of the algae solution is about 10 6 cell / L. The culture conditions are: light 2000lx, temperature 27°C, light-dark ratio 14:10.

[0044] 2. Acidification of the culture solution: acidify the solution on the second day of cultivation, and set the pH to 6.0.

[0045] 3. Continuous ventilation: the frequency of ventilation is 2 times / day, at 10:00 and 16:00 every day. Each aeration was stopped when the pH of the solution reached 6.0.

[0046] 4. Monitor the pH and chlorophyll a of the culture solution: measure the pH and chlorophyll a of the solution before and after daily aeration. The results showed that the pH value of the algae liquid in the aci...

Embodiment 2

[0049] In this test, one blank tank (non-ventilated tank) and three acidizing tanks (ventilated tank) are set.

[0050] 1. Cultivation of algae species: insert 1L of Microcystis aeruginosa species in the logarithmic growth phase into 10L of M11 culture solution (the formula of M11 culture solution is such that 1L of deionized water contains NaNO 3 100mg, K 2 HPO 4 10mg, MgSO 4 ·7H 2 O75mg, CaCl 2 2H 2 O40mg, Na 2 CO 3 20mg, Ferric Citrate 6mg, Na 2 EDTA·2H 2 O1 mg. In this experiment, high-purity water was used instead of deionized water to eliminate the interference of bacteria to the greatest extent) in a tank (size: 40mm×25mm×30mm), the concentration of algae liquid was about 10 6 cell / L. The culture conditions are: light 2000lx, temperature 27°C, light-dark ratio 14:10.

[0051] 2. Acidification of the culture solution: On the second day of the culture, the solutions in the three tanks were acidified, and the pHs were set to 5.5, 6.0 and 6.5 respectively.

...

Embodiment 3

[0056] In this test, one blank tank (non-ventilated tank) and three acidizing tanks (ventilated tank) are set.

[0057] 1. Cultivation of algae species: insert 1L of Microcystis aeruginosa species in the logarithmic growth phase into 10L of M11 culture solution (the formula of M11 culture solution is such that 1L of deionized water contains NaNO 3 100mg, K 2 HPO 4 10mg, MgSO 4 ·7H 2 O75mg, CaCl 2 2H 2 O40mg, Na 2 CO 3 20mg, Ferric Citrate 6mg, Na 2 EDTA·2H 2 O1 mg. In this experiment, high-purity water was used instead of deionized water to eliminate the interference of bacteria to the greatest extent) in a tank (size: 40mm×25mm×30mm), the concentration of algae liquid was about 10 6 cell / L. The culture conditions are: light 2000lx, temperature 27°C, light-dark ratio 14:10.

[0058] 2. Acidification of the culture solution: On the 15th day of the culture (the time when it entered the stable phase), the solutions in the three tanks were acidified, and the pHs wer...

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Abstract

The invention discloses a method for inhibiting the growth of cyanobacteria, comprising the following steps of: 1, acidification on a water body to be processed: charging CO2 gas into an inoculated alga solution, and acidifying the alga solution by utilizing the characteristic that CO2 and water react to generate H<+> so that the pH value of the alga solution can reach a preset acidity; 2, continuous CO2 charging: regularly charging CO2 into the alga solution in the daily light period so that the pH value of the alga solution can reach the preset acidity after the acidification process starts; and 3, charging pause: stopping CO2 charging when the alga solution is changed to be almost clear from aqua before CO2 charging and the value of chlorophyll a is close to 0 or the inhibition effect does not change remarkably with the acidifying treatment time, wherein the step 2 further comprises a step of monitoring the pH value of the alga solution and the chlorophyll a: measuring the pH value of the alga solution and the chlorophyll a before CO2 charging every day. By utilizing a method of using CO2 to acidify alga growth environment to inhibit the growth of the cyanobacteria, the invention can effectively inhibit the growth of the cyanobacteria with strong practical operability and does not have the problem of secondary pollution.

Description

technical field [0001] The invention relates to the technical field of eutrophic lake control, in particular to the technical field of inhibiting the growth of cyanobacteria. technical background [0002] In recent years, with the continuous development of society, the rapid increase of population and the development and utilization of lake resources, the water resource control is relatively insufficient, and the pollution of lakes has become increasingly serious, especially in key lakes such as Dianchi Lake, Chaohu Lake and Taihu Lake. The outbreak of algae bloom is a main feature of serious lake pollution. The outbreak of algae blooms makes the water taste fishy and smelly, the DO in the deep water layer decreases, the release of toxic substances affects the quality of water supply, increases the cost of water purification, and changes the aquatic ecology. Among them, the most likely to form algal blooms, the most widely affected, and the most toxic are Cyanophyta algae. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F1/66
Inventor 冯传平郝春博王欣
Owner CHINA UNIV OF GEOSCIENCES (BEIJING)
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