Method and device for culturing cells such as stem cells and the like for therapy in mobile carrier mode

A technology for moving carriers and stem cells, which can be used in tissue cell/virus culture devices, animal cells, vertebrate cells, etc. It can solve the problems of easy contamination, complicated operation, cell damage, etc., to simplify the operation process and improve work efficiency. , the effect of reducing the risk of infection

Active Publication Date: 2013-08-21
上海坤爱生物科技股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The first object of the present invention is to provide a device for cultivating stem cells in a mobile carrier type, so as to solve the technical problems of cell damage, cumbersome operation and easy bacterial contamination in the existing culture device
[0005] The second object of the present invention is to provide a mobile carrier-type method for culturing cells for treatment, so as to solve the technical problems of cell damage in existing culture devices, cumbersome operation and easy contamination

Method used

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  • Method and device for culturing cells such as stem cells and the like for therapy in mobile carrier mode
  • Method and device for culturing cells such as stem cells and the like for therapy in mobile carrier mode
  • Method and device for culturing cells such as stem cells and the like for therapy in mobile carrier mode

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Embodiment Construction

[0014] The core of this patent lies in: a mobile carrier-type device and method for culturing stem cells and other therapeutic cells, using the mobile carrier as a support for cell growth. This method avoids the damage to the cells during the passaging process in the traditional culture method, and at the same time, the method is simple to operate, not easy to infect bacteria, and also provides the possibility for large-scale culture.

[0015] see figure 1 , which is a schematic diagram of the principle structure of the cell culture device of the present invention. It includes: inoculation port 1 for inoculation, sampling port 2 for detecting cell culture status during culture, filling port 3 for loading and taking out the movable carrier, and intercepting device to prevent the movable carrier from flowing out of the culture device 5. Movable carrier 6 as a support for cell growth.

[0016] see figure 2 with image 3 , The blocking device 5 may be a built-in blocking devi...

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Abstract

The invention discloses a device and a method for culturing cells such as stem cells and the like for therapy in a mobile carrier mode. The method comprises the following steps of: adding a culture medium in a cell culture device through a filling hole to provide nutrition for culture and amplification of the cells such as the stem cells and the like for therapy, and inoculating mobile carriers with cells or cells in the cell culture device through an inoculation hole to provide seed cells for culture and amplification of the cells such as the stem cells and the like for therapy; filling a proper amount of no-load mobile carriers in the cell culture device to provide an expanded support space for culture and growth of the cells; when cells are fully grown on partial or all mobile carriersafter culture for a preset time, adsorbing the mobile carriers through a sampling hole by using an adsorption tool, and dividing the adsorbed mobile carriers into a plurality of equal parts as subculture seeds; and when the last subculture is completed, taking out all the mobile carriers with cells from the culture device, and performing enzymolysis digestion and blowing to harvest the cells.

Description

technical field [0001] The invention relates to a cell culture system, in particular to a mobile carrier-type device and method for culturing cells for treatment such as stem cells. Background technique [0002] Since James Thomson obtained embryonic stem cells from human in vitro fertilized eggs in 1998, and they can be stably cultured in vitro, cell culture devices for stem cells and other therapeutic cells (such as: culture bottles, culture bags, etc.) ) is relatively fixed, and the cultivation process and methods are becoming more and more mature. Their common feature is that stem cells and other therapeutic cells are inoculated in cell culture devices such as culture flasks and culture bags. After culturing under suitable conditions for a certain period of time, the culture device needs to be opened to remove non-adherent cells by changing the medium. After the adherent cells reach a certain number, the culture device is opened again, and the cells are recovered by en...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/0735C12N5/074C12N5/0775C12N5/0789C12N5/0797C12M3/00
Inventor 齐瀚实郑琛李巍巍
Owner 上海坤爱生物科技股份有限公司
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