Metal-modified photonic crystal biological detection film as well as preparation method and application thereof

A photonic crystal and metal modification technology, applied in biochemical equipment and methods, biological testing, microbial measurement/inspection, etc., can solve the problems of expensive, complex instruments, and restrictions on the pace of practical use, and achieve enhanced sensitivity, high Effects of Sensitive Bioassays

Active Publication Date: 2011-05-25
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

From the perspective of improving the instrument, in order to meet the high-sensitivity test requirements, it is necessary to strictly control the experimental conditions, minimize the background fluorescence, use a complex optical system, and have particularly high requirements for the quality of the detector, so the instrument is expensive. The experimental process has strict requirements, and it is difficult to popularize and apply this method, which seriously restricts the pace of its practical application.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] 1. Prepare a solution of polystyrene latex particles with a monodisperse particle size of 205nm at a mass concentration of 0.1% with water, and sufficiently sonicate to make the latex particles uniformly dispersed. Weigh 8.0g NaCl, 3.23g Na 2 HPO 4 12H 2 O and 0.45 g NaH 2 PO 4 2H 2 O, dissolved in 800ml of distilled water, adjusted the pH value of the solution to 7.4 with HCl, and finally added distilled water to make up to 1L to obtain 0.01M phosphate buffered saline (PBS).

[0063] 2. Take a clean carrier film (ordinary glass) as the substrate, insert the substrate vertically into the solution containing polystyrene latex particles obtained in step 1, at a temperature of 50°C and a humidity of 50% under constant conditions, Deposit the polystyrene latex particles obtained in step 1 on the substrate by vertical deposition, and self-assemble and arrange on the substrate to prepare polystyrene photonic crystals. Polystyrene photonic crystals are periodic dielectric...

Embodiment 2

[0069] 1. Prepare a solution of poly(styrene-methyl methacrylate-acrylic acid) latex particles with a monodisperse particle size of 0.2% and a poly(styrene-methyl methacrylate-acrylic acid) latex particle solution with a mass concentration of 0.2% in water, and sufficiently ultrasonicate to make the latex particles uniformly dispersed.

[0070] 2. With a clean quartz plate as the substrate, insert the substrate vertically into the solution containing poly(styrene-methyl methacrylate-acrylic acid) latex particles obtained in step 1, at a temperature of 50°C and a humidity of 50 Under the constant condition of %, the latex liquid that step 1 obtains is deposited on the substrate by the vertical deposition method, self-assembled arrangement on the substrate prepares poly(styrene-methyl methacrylate-acrylic acid) photonic crystal, poly(phenylene Ethylene-methylmethacrylate-acrylic acid) photonic crystals are opal structures with a periodic dielectric structure with a photonic band ...

Embodiment 3

[0076] 1. Prepare a solution of polymethyl methacrylate latex particles with a monodisperse particle size of 354nm and a mass concentration of 3% with water, and sufficiently ultrasonicate to make the latex particles uniformly dispersed.

[0077] 2. With a clean polyvinyl chloride sheet as the substrate, insert the substrate vertically into the solution containing polymethyl methacrylate latex particles obtained in step 1, under the constant conditions of 80°C and 80% humidity , deposit the latex solution obtained in step 1 on the substrate by vertical deposition method, and self-assemble and arrange on the substrate to prepare polymethyl methacrylate photonic crystal. Polymethyl methacrylate photonic crystals are opal structures with periodic dielectric structures with photonic band gaps.

[0078] 3. Using ethanol as a solvent and concentrated hydrochloric acid as a catalyst to hydrolyze tetraethyl orthosilicate and improve it to prepare SiO 2 Sol. The specific method is as...

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Abstract

The invention relates to a metal-modified photonic crystal biological detection film as well as a preparation method and application thereof. The film is a metal-modified photonic crystal film marked of a biological function substance which is marked by a fluorescence labeling molecule, wherein the biological function substance marked by the fluorescence labeling molecule is a biological function substance marked by a single fluorescence labeling molecule or a receptor fluorescence labeling pair. By the utilization of the characteristics of photonic crystal enhancement and metal surface enhancement, the adaptation of the fluorescence labeling biological function substance, and the specific responsiveness and the specific affinity of the fluorescence labeling biological function substance on a target biological substance, the target biological substance and the biological function substance generate response, thereby the illumination change of the biological function substance marked by the fluorescence labeling molecule is caused. Moreover, the sensitivity of a system for detecting the target biological substance can be effectively enhanced by reading and identifying fluorescent signals of the fluorescence labeling molecule through photonic crystal enhancement and metal surface enhancement, consequently the high-sensitivity biological detection can be realized.

Description

technical field [0001] The invention belongs to the field of detecting multiple proteins and genes in biological samples, and in particular relates to a metal-modified photonic crystal bio-detection film and a preparation method thereof, and the use of the metal-modified photonic crystal to detect biological substances. Background technique [0002] The rapid development of life sciences has raised a large number of new topics for analytical chemistry, currently focusing on the analysis of biological macromolecules such as peptides, proteins, and nucleic acids, the analysis of biological drugs, the analysis of ultra-trace and ultra-trace biologically active substances, and even the analysis of microorganisms. Therefore, bioanalysis has become one of the most important frontier fields in the development of modern analytical chemistry. In order to meet the requirements of this situation, many analytical chemists are making continuous efforts to develop new analytical methods a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N33/74C12Q1/68C12Q1/37C12Q1/00G02F1/00
Inventor 李明珠沈为之宋延林
Owner INST OF CHEM CHINESE ACAD OF SCI
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