Metal-modified photonic crystal biological detection film as well as preparation method and application thereof

Abstract

The invention relates to a metal-modified photonic crystal biological detection film as well as a preparation method and application thereof. The film is a metal-modified photonic crystal film marked of a biological function substance which is marked by a fluorescence labeling molecule, wherein the biological function substance marked by the fluorescence labeling molecule is a biological function substance marked by a single fluorescence labeling molecule or a receptor fluorescence labeling pair. By the utilization of the characteristics of photonic crystal enhancement and metal surface enhancement, the adaptation of the fluorescence labeling biological function substance, and the specific responsiveness and the specific affinity of the fluorescence labeling biological function substance on a target biological substance, the target biological substance and the biological function substance generate response, thereby the illumination change of the biological function substance marked by the fluorescence labeling molecule is caused. Moreover, the sensitivity of a system for detecting the target biological substance can be effectively enhanced by reading and identifying fluorescent signals of the fluorescence labeling molecule through photonic crystal enhancement and metal surface enhancement, consequently the high-sensitivity biological detection can be realized.

Description

technical field

[0001] The invention belongs to the field of detecting multiple proteins and genes in biological samples, and in particular relates to a metal-modified photonic crystal bio-detection film and a preparation method thereof, and the use of the metal-modified photonic crystal to detect biological substances. Background technique

[0002] The rapid development of life sciences has raised a large number of new topics for analytical chemistry, currently focusing on the analysis of biological macromolecules such as peptides, proteins, and nucleic acids, the analysis of biological drugs, the analysis of ultra-trace and ultra-trace biologically active substances, and even the analysis of microorganisms. Therefore, bioanalysis has become one of the most important frontier fields in the development of modern analytical chemistry. In order to meet the requirements of this situation, many analytical chemists are making continuous efforts to develop new analytical methods a...