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Method for distinguishing and sorting cells and device therefor

A cell and sorting technology, which is applied in biochemical equipment and methods, biochemical cleaning devices, enzymology/microbiology devices, etc., can solve the problems of low analysis accuracy and inability to clearly identify cell size, and achieve accuracy improvement, Effects that contribute to differentiation/induction

Active Publication Date: 2015-04-22
雅马拓科学株式会社
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] Conventional cell analysis devices, such as flow cytometers, analyze cell size based on forward scattered light information, but there is a problem that the analysis accuracy is low and cell size cannot be clearly identified

Method used

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  • Method for distinguishing and sorting cells and device therefor
  • Method for distinguishing and sorting cells and device therefor
  • Method for distinguishing and sorting cells and device therefor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0088] Use according to Figure 4 The cell sorting device that performs cell sorting according to the measurement principle shown above irradiates laser light to HeLa cells passing through the flow cell, detects the light transmitted through the cells, the light transmitted through the cells, and the light scattered laterally by the cells to create transmitted light. A histogram formed by the peaks of information. figure 1 Represents a histogram formed using transmitted light information. Such as figure 1As shown, the G1 phase, S phase, and M phase of HeLa cells are respectively determined by figure 1 a. figure 1 b. figure 1 c measurement and analysis.

[0089] figure 1 The peak of transmitted light information shown on the horizontal axis reflects information on cell morphology. As the horizontal axis moves to the right, the size becomes larger; as the horizontal axis moves to the left, the size becomes smaller. The vertical dashed boundary line roughly correspo...

Embodiment 2

[0092] The same can be used according to Figure 4 The cell sorting device that performs cell sorting according to the measurement principle shown above irradiates laser light to HeLa cells passing through the flow cell, detects the light transmitted through the cells and the light scattered laterally by the cells, and creates transmitted light and side scattered light information The resulting analytical distribution plot. image 3 A graph representing its image. Such as image 3 As shown, the G1 phase, S phase, and M phase of HeLa cells are respectively determined by image 3 a. image 3 b. image 3 c measurement and analysis.

[0093] image 3 The transmitted light information shown on the horizontal axis reflects the information on the cell morphology, and the side scattered light information shown on the vertical axis reflects the information on the internal structure of the cell. As the horizontal axis moves to the right, the shape becomes larger; as the horizonta...

Embodiment 3

[0096] Figure 4 Shows the configuration of the measurement section capable of measuring transmitted light information and side scattered light information. By using a cell sorting device equipped with this measurement unit, using measurement points provided in the direction of cell flow, in addition to measuring fluorescence information, transmitted light information, and side scattered light information of cells, multiple measurement points can also be used. The transmitted light information is used to calculate the flow velocity information of the cells.

[0097] Figure 5 It shows the configuration of a sorting unit capable of sorting one to a plurality of cells from a predetermined cell population into predetermined wells. By using the cell sorting device of the present invention equipped with this sorting unit, the time for the cells to be sorted to reach the dispensing nozzle can be known based on the flow velocity information, so the dispensing nozzle is separated fr...

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Abstract

A method for distinguishing and sorting cells characterized by comprising distinguishing and sorting a specific cell mass or a part of the cells in the cell mass with the use of transmitted light data reflecting the morphological characteristics of the cells such as size and shape optionally together with side-scattering light data reflecting the characteristics of the internal structure of the cells. The part of the cells in the specific cell mass as described above are at the G1 stage or at a part of the M stage in the cell cycle. A part of the cells at the G1 stage are referred to as the left bottom line in an analytical dispersion diagram of the cells wherein the abscissa indicates the transmitted light data, while a part of the cells at the M stage are referred to as the right bottom line in the analytical dispersion diagram of the cells wherein the abscissa indicates the transmitted light data.

Description

technical field [0001] The present invention relates to a method for identifying and sorting cells, in particular to a method for identifying and sorting cells using only transmitted light information, or using transmitted light information and side scattered light information, and a device thereof, wherein, The above transmitted light information mainly reflects at least one of cell morphological features including cell size, shape, etc., and cell internal structure features including cell nucleus, cytoplasm, etc., and the above side scattered light information reflects the characteristics of cell internal structure. Background technique [0002] A method called flow cytometry is used in which cells are analyzed one by one by flowing a cell suspension at high speed and performing measurement by irradiating laser light. It is known that when a flow cytometer is used, the relative size, shape, and internal structure of each cell are different, and then fluorescently labeled t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00
CPCG01N2015/1493G01N15/147G01N2015/1438G01N2015/1497
Inventor 高桥亨月井健徐杰
Owner 雅马拓科学株式会社