Reagent kit for detecting Fumonisin B1 of genetically engineered single-chain antibody and method thereof

Abstract

The invention provides a reagent kit for detecting Fumonisin B1 of genetically engineered single-chain antibody and method thereof; in the reagent kit of the invention, the reagent comprises: sample extracting solution, a standard reagent, an enzyme labeled antigen reagent, washing solution, a BSA (bovine serum albumin) regent, a chromogenic substrate and stopping solution; the content of FB1 toxins in the sample is calculated by the following steps of sample processing, regent balancing, washing, adding sampling, washing, color developing and stopping. The Fumonisin B1 single-chain antibody adopted by the reagent kit provided by the invention can be effectively expressed in colon bacillus, and can be produced in a large scale; the producing process is simple, convenient and easy; the time, labor and money are saved. Using the reagent kit in the invention for detecting the Fumonisin B1, whether the sample is polluted by the Fumonisin B1 or not can be determined, and the content of the Fumonisin B1 is calculated in 1.5-2h. The reagent kit is convenient and fast in use, and low in cost.

Description

Detection of fumonisin B by a genetically engineered single-chain antibody 1 kits and methods technical field The invention relates to a detection kit for detecting mycotoxins by using a genetically engineered single-chain antibody, more particularly to a kit for detecting fumonisin B1 (FumonisinB1, FB1) by using a genetically engineered single-chain antibody, and further relates to the use of the reagent The method of cassette detection of FB1. Background technique Fumonisin is a water-soluble mycotoxin, and its pollution is widespread in the world. It mainly pollutes food crops and feed, especially corn and its products. Fumonisin has high thermal stability and is not easy to be destroyed. It has various toxicity and carcinogenicity to humans and animals, such as neurotoxicity and pulmonary toxicity. It mainly damages the liver and kidneys and seriously endangers the health of humans and animals. Currently, the antibodies used for immunodetection of fumonisins are all ...

AI Technical Summary

Problems solved by technology

In order to overcome the time-consuming, labor-intensive and cost-intensive shortcomings of the existing monoclonal antibody production, the present invention provides a kit and method for detecting fumonisin B1 by a genetically engineered single-chain antibody, which makes the detection process of fumonisin B1 simple and convenient Easy to operate, save time, effort and money,

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