Multi-epitope artificial antigen of plasmodium falciparum and application thereof
A technology of Plasmodium falciparum and its application, which is applied in the field of immune preparations against Plasmodium falciparum infection and the preparation of diagnostic reagents for Plasmodium falciparum infection, can solve problems such as antigenic variation, and achieve the effect of high inhibition efficiency and high immunogenicity
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[0035] Materials, Methods and Results:
[0036] 1. Epitope gene synthesis
[0037] By consulting the literature, after analysis, it is determined to select the epitope sequences in Table 1, and at the same time, a specific spacer sequence is incorporated at both ends of each epitope gene sequence (introducing a start codon into the N-terminal epitope gene sequence, and introducing a start codon at the C-terminal Introducing a stop codon into the epitope gene sequence), according to a specific sequence (such as figure 2 ), the full-length gene sequence of M.RCAg-3 was synthesized by Shanghai Sangong Company with common codons, such as figure 1 SEQ ID No: 1 in.
[0038] Table 1. Epitope information table (sequences correspond to SEQ ID No: 5-14 respectively)
[0039] gauge
Antigen comes
source
epitope sequence
gauge
Types of
Amino
acid number
head
refer to
literature
E1
RESA
MQTLWDEIMD...
PUM
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Description
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Application Information
- IPC
- C12N15/11; C12N15/63; C12N1/21; C07K14/445; C07K16/20; A61K39/015; A61K31/7088; A61K39/395; A61P33/06; G01N33/569; C12R1/19
- CPC
- Y02A50/30
- Inventors
- ηζ; ζ²δΉ ι«