Multi-epitope artificial antigen of plasmodium falciparum and application thereof
A technology of Plasmodium falciparum and its application, which is applied in the field of immune preparations against Plasmodium falciparum infection and the preparation of diagnostic reagents for Plasmodium falciparum infection, can solve problems such as antigenic variation, and achieve the effect of high inhibition efficiency and high immunogenicity
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Example Embodiment
[0035] Materials, methods and results:
[0036] 1. Epitope gene synthesis
[0037] By consulting the literature, after analysis, it is determined to select the epitope sequence in Table 1. At the same time, a specific spacer sequence is incorporated at both ends of each epitope gene sequence (introduction of the start codon in the N-terminal epitope gene sequence, and the C-terminal Introduce a stop codon into the epitope gene sequence), according to a specific sequence (such as figure 2 ), the full-length gene sequence of M.RCAg-3 was synthesized by Shanghai Shenggong Company with universal codons, such as figure 1 SEQ ID No:1 in the.
[0038] Table 1. Epitope information table (sequence corresponds to SEQ ID No: 5-14)
[0039] gauge
[0040] Two, carrier transformation
[0041] Original vector: pET-30a-c(+) was purchased from Novagen, see Figure 5 ;
[0042] Modified primers of the original vector:
[0043] Upstream primer (SEQ ID No: 3):
[0044] 5’CCCTCTAGAAATAATTTTGTTTAACTTTAAGA...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap