Method for preparing peanut dietary fibers by using microbial fermentation method
A microbial fermentation method and dietary fiber technology, applied in the field of food processing, can solve the problems of lack of systematic research and poor utilization of resources
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Embodiment 1
[0028] Take 0.3ml of liquid PDY first medium with a sterile pipette, drop it into the Aspergillus niger freeze-dried strain tube, shake gently to dissolve the freeze-dried bacteria into a suspended liquid, take about 0.2ml of the bacterial suspension, transfer On the first slant of PDA medium, culture it in an incubator at 27°C for 3 days to obtain activated strains; use an inoculation loop to pick 1 ring of activated strains on the second slope of PDA medium, and incubate at 27°C in the incubator Expand the culture for 3 days to obtain the expanded culture strains; rinse the strains on the second slant of the PDA medium with sterile water to form a bacterial suspension, and then insert the bacterial suspension into the 100ml PDY second medium. Shake well at 27°C at 150r / min and vibrate for 3 days in a triangular flask to obtain the seed culture solution of the fungus; take peanut shells that are free from mildew and moth-eaten, wash them with running water, and dry them in a b...
Embodiment 2
[0030] Take 0.4ml of liquid PDY first medium with a sterile pipette, drop it into the tube of Trichoderma viride freeze-dried strains, shake gently to dissolve the freeze-dried bacteria into a suspended liquid, take about 0.2ml of the bacterial suspension, and transfer to Connect it on the first slope of PDA medium, and cultivate it in an incubator at 29°C for 4 days to obtain activated strains; pick up a ring of activated strains with an inoculation loop and place it on the second slope of PDA medium, and place it in an incubator at 29°C Expand the culture for 4 days to obtain the expanded culture strains; rinse the strains on the second slant of the PDA medium with sterile water to form a bacterial suspension, and then insert the bacterial suspension into the PDY second medium containing 100ml Shake well at 29°C and culture at 150r / min for 4 days to obtain the seed culture solution of the fungus; wash the peanut stalks without mildew and insects with running water, and dry th...
Embodiment 3
[0032] Take 0.3ml of liquid PDY first medium with a sterile pipette, drop it into the tube of Rhizopus oryzae freeze-dried strains, shake gently to dissolve the freeze-dried bacteria into a suspended liquid, take about 0.2ml of bacterial suspension, transfer to Connect it to the first slant of the PDA medium, and culture it in an incubator at 30°C for 4 days to obtain the activated strain; use an inoculation loop to pick 1 ring of the activated strain on the second slope of the PDA medium, and place it in the incubator at 30°C Expand the culture for 4 days to obtain the expanded culture strains; rinse the strains on the second slant of the PDA medium with sterile water to form a bacterial suspension, and then insert the bacterial suspension into the PDY second medium containing 100ml Shake well at 30°C and shake at 150r / min for 4 days to obtain the seed culture solution of the fungus; take the peanut roots without mildew and moth-eaten, wash them with running water, and dry the...
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