A method for preparing wheat bran food fibre using ultrasound wave assistance enzymolysis
A technology of wheat bran dietary fiber and auxiliary enzymolysis, which is applied in the fields of functional food additives, nutritional health care, and agricultural and sideline product development, can solve problems such as unsatisfactory, and achieve the purpose of preventing constipation, increasing economic and social benefits, and preventing heart disease. Effect
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Embodiment 1
[0028] Embodiment 1: The present invention utilizes ultrasonic-assisted enzymolysis to prepare wheat bran dietary fiber and adopts the following process steps:
[0029] In the embodiment of the present invention, the wheat bran is first sieved with a pulverizer to remove impurities and part of the starch, and pulverized and ground into a 30-mesh sample.
[0030] Accurately weigh 50 g of wheat bran under a 35-mesh sieve, suspend it in 400 ml of water, control the temperature of the solution at 30 °C, and perform ultrasonic treatment with an ultrasonic cell disruptor, ultrasonic power 300 W, ultrasonic time 40 min.
[0031] Use 1mol / l hydrochloric acid solution to adjust pH value: 5.6, then add 0.1ml high temperature resistant α-amylase, use high temperature resistant α-amylase to remove starch in wheat bran, the mixture is stirred in a boiling water bath for 10min, the boiling water temperature It is: 95 ℃; use iodine solution to detect whether the starch has reacted completely...
Embodiment 2
[0037] Embodiment 2: the present invention utilizes ultrasonic-assisted enzymolysis to prepare wheat bran dietary fiber and adopts the following process steps:
[0038] In the embodiment of the present invention, a pulverizer is used to grind the wheat bran into a 40-mesh sample.
[0039] Accurately weigh 50 g of wheat bran under a 35-mesh sieve, suspend it in 500 ml of water, control the temperature of the solution at 40 °C, and use an ultrasonic cell disruptor to process it with an ultrasonic power of 400 W and an ultrasonic time of 60 min.
[0040] Use 1mol / l hydrochloric acid solution to adjust the pH value to 5.6, then add 0.2ml of high temperature resistant α-amylase, stir the mixture in a boiling water bath for 10min, the boiling water temperature is: 98 ℃, use iodine solution to check whether the starch has reacted completely, if it turns blue, then Continue the reaction until the iodine solution does not turn blue.
[0041] After the above suspension was cooled to 50°C...
Embodiment 3
[0044] Embodiment 3: the present invention utilizes ultrasonic-assisted enzymolysis to prepare wheat bran dietary fiber and adopts the following process steps:
[0045] In the embodiment of the present invention, a pulverizer is used to grind the wheat bran into a 50-mesh sample.
[0046] Accurately weigh 50 g of wheat bran under a 35-mesh sieve, suspend it in 600 ml of water, control the temperature of the solution at 50 °C, and use an ultrasonic cell disruptor to process it with an ultrasonic power of 500 W and an ultrasonic time of 8 min.
[0047] Use 1mol / l hydrochloric acid solution to adjust the pH value to 5.6, then add 0.3ml of high temperature resistant α-amylase, stir the mixture in a boiling water bath for 10min, use iodine solution to check whether the starch has reacted completely, if it turns blue, continue the reaction until the iodine solution detects until it doesn't turn blue.
[0048] After the suspension was cooled to 50°C, the pH value was adjusted to 9.0...
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