Two fungi for promoting seeds of Dendrobium to sprout

A technology of plant seeds, fungi, applied in the production of

Active Publication Date: 2011-07-20
INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

They can be used in the artificial propagation and production of a variety of rare and endangered medicinal Dendrobium plants, increase the yield of Dendrobium, solve the problem of shortage of medicinal resources of

Method used

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  • Two fungi for promoting seeds of Dendrobium to sprout

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Effects of pw-3 on germination of Dendrobium candidum seeds

[0011] Sow the seeds of Dendrobium officinale on the oat medium that has been overgrown with pw-3 fungal mycelium, and use the seeds sown on the sterile oat medium as a control, and cultivate them in the cultivation room at a temperature of 23-27°C for three Seed germination was investigated at 1 month. The classification standards of Orchidaceae seed development grades are shown in Table 1. The result is: the seed germination rate of Dendrobium candidum inoculated with pw-3 fungus is 69.3%, and the germination rate of Dendrobium candidum seed without inoculated fungus is 24.6%; , the control group only developed to the second stage, that is, the seeds swelled and broke the skin, and no further development was seen.

[0012] Table 1 Orchidaceae seed development grade grading standard

[0013]

Embodiment 2

[0015] Effects of pw-3 on Germination of Dendrobium nobile Seeds

[0016] Sow the seeds of Dendrobium nobile on the oat medium that has been covered with pw-3 fungal mycelium, and use the seeds sown on the sterile oat medium as a control, and cultivate them in a culture room at a temperature of 23-27°C. Seed germination was investigated at three months. The result is: the seed germination rate of Dendrobium nobile inoculated with pw-3 fungus is 56.6%, and the seed germination rate of Dendrobium nobile inoculated with fungus is 31.7%; One or two leaves grew and developed into seedlings, while the control group only developed to the second level, that is, the seeds swelled and broke the skin, and no further development was seen.

Embodiment 3

[0018] Germination Effect of pw-3 on Dendrobium Copperskin Seeds

[0019] Sow the seeds of Dendrobium nodule on the oat medium that has been covered with pw-3 fungal mycelium, and use the seeds sown on the sterile oat medium as a control, and cultivate them in the cultivation room at a temperature of 23-27°C. Seed germination was investigated at two months. The result is: the seed germination rate of Dendrobium copperskin seed that is inoculated with pw-3 fungus is 92.0%, and the seed germination rate of Dendrobium copperskin that is not inoculated with fungus is 54.3%; One or two leaves grew and developed into seedlings, while the control group only developed to the second level, that is, the seeds swelled and broke the skin, and no further development was seen.

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Abstract

The invention discloses two fungi for promoting seeds of Dendrobium to sprout, which have great application value on sexual propagation of Dendrobium, mass production of seedlings and sustainable utilization of resources.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to two fungi which can promote the germination of the seeds of Dendrobium plants in the family Orchidaceae, be used for the reproduction and production of the Dendrobium plants, and protect the plant resources of the genus. Background technique [0002] Orchidaceae (Orchidaceae) is one of the large families of angiosperms. There are about 700 genera and nearly 20,000 species in the world, many of which are rare and endangered plants. All wild orchids are included in the scope of protection of the Convention on International Trade in Endangered Species of Wild Fauna and Flora, accounting for more than 90% of the plants to be protected by the convention, and are the "flagship" group in plant protection. Dendrobium is the second largest genus of Orchidaceae, many of which have high medicinal and ornamental value. According to the 2005 edition of "The Pharmacopoeia of the People...

Claims

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Application Information

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IPC IPC(8): C12N1/14C05F11/08C12R1/645
Inventor 郭顺星王卉陈娟陈晓梅孟志霞
Owner INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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