Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Precursor liposome of propolis flavone and preparation method thereof

A technology of proliposome and propolis flavonoids is applied in the directions of liposome delivery, pharmaceutical formulations, medical preparations of non-active ingredients, etc., can solve problems such as unseen, achieve convenient storage, good health care effect, and enhance stability sexual effect

Inactive Publication Date: 2011-08-03
射阳县庆缘康蜂产品厂
View PDF0 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] So far, there are no reports about the preparation of propolis flavone proliposomes at home and abroad

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Weigh 200 mg of lecithin and 20 mg of cholesterol and dissolve in 30 mL of anhydrous ether (organic phase); weigh 20 mg of propolis flavonoids and dissolve in 10 mL of deionized water (water phase). Mix the obtained organic phase with the water phase, and form a stable and uniform water-in-oil emulsion after ultrasonic treatment, transfer it to a 250 mL rotary evaporating flask, and remove ether by rotary evaporation in a water bath at 35°C, and there will be a gel in 5-10 minutes Formation, gel collapsed as rotary evaporation continued, and reduced pressure was maintained for 30 minutes to remove residual ether. Add 10 mL of an aqueous solution containing 250 mg Tween 80 to the evaporating flask, and hydrate with rotation in a water bath at 35°C for 30 minutes. After hydration, the propolis flavone nano liposome dispersion is obtained. Pipette 20 mL of the propolis flavone nanoliposome dispersion into a watch glass. The dispersion contains 200 mg of phospholipids, and...

Embodiment 2

[0023] Weigh 200 mg of lecithin and 20 mg of cholesterol and dissolve in 30 mL of anhydrous ether (organic phase); weigh 20 mg of propolis flavonoids and dissolve in 10 mL of deionized water (water phase). Mix the obtained organic phase with the water phase, and form a stable and uniform water-in-oil emulsion after ultrasonic treatment, transfer it to a 250 mL rotary evaporating flask, and remove ether by rotary evaporation in a water bath at 35°C, and there will be a gel in 5-10 minutes Formation, gel collapsed as rotary evaporation continued, and reduced pressure was maintained for 30 minutes to remove residual ether. Add 10 mL of an aqueous solution containing 250 mg Tween 80 to the evaporating flask, and hydrate with rotation in a water bath at 35°C for 30 minutes. After hydration, the propolis flavone nano liposome dispersion is obtained. Pipette 20 mL of the propolis flavonoid nanoliposome dispersion into a watch glass. The dispersion contains 200 mg of phospholipids, a...

Embodiment 3

[0025] Weigh 300 mg of lecithin and 60 mg of cholesterol, dissolve in 30 mL of anhydrous ether (organic phase); weigh 30 mg of propolis flavonoids and dissolve in 10 mL of deionized water (water phase). Mix the obtained organic phase with the water phase, and form a stable and uniform water-in-oil emulsion after ultrasonic treatment, transfer it to a 250mL rotary evaporator flask, remove ether by rotary evaporation in a water bath at 40°C, and a gel will form in 5-10 minutes , the gel collapsed as the rotary evaporation continued and the reduced pressure was maintained for 30 minutes to remove residual ether. Add 20 mL of deionized aqueous solution containing 300 mg Tween 80 to the evaporating flask, and hydrate with rotation in a water bath at 40°C for 30 minutes. Propolis flavone nano liposome dispersion liquid is obtained after hydration. The resulting dispersion was stored in a refrigerator at 4°C. Pipette 20 mL of the propolis flavone nanoliposome dispersion into a watc...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a precursor liposome of propolis flavone and a preparation method thereof. The method is characterized by comprising the following steps of: preparing a nanometer liposome suspension of the propolis flavone by a reverse-phase evaporation method, and freeze drying the nanometer liposome suspension of the propolis flavone to prepare the precursor liposome of the propolis flavone; and preparing a nanometer liposome of the propolis flavone with Tween 80 by taking the propolis flavone as cores and lecithin and cholesterol as membranes, and freeze drying the prepared nanometer liposome of the propolis flavone by the reverse-phase evaporation method to obtain the precursor liposome of the propolis flavone. Due to the adoption of the technical scheme, the stability of the propolis flavone in a strongly-acidic system of gastric acid can be enhanced to improve biological availability and achieve better health-care effect. The precursor liposome of the propolis flavone prepared by the freeze drying method can be convenient to store and convey and also can be applied in wide food systems.

Description

technical field [0001] The invention belongs to the technical field of bee product processing technology, and in particular relates to a propolis flavone proliposome and a preparation method thereof. Background technique [0002] Propolis is a kind of colloidal solid with aromatic flavor, which is processed by honey bees from the resin collected from plant buds or broken parts of tree trunks, and mixed with the secretions of their mandibular glands and beeswax. Propolis has the functions of antibacterial, anti-corrosion, anti-virus, anti-oxidation, promoting blood circulation and removing stasis, softening blood vessels, lowering blood fat, anti-tumor, enhancing human immunity, and promoting tissue regeneration; [0003] The composition of propolis is quite complex, among which flavonoids are the main active substances. Propolis has the reputation of "treasure house of flavonoids". The flavonoids that have been isolated and identified from propolis include chrysin, apigenin...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K35/64A61K9/127A61K47/34A61P3/06A61P9/00A61P31/04A61P31/12A61P35/00A61P37/04A61P39/06A61P43/00A61K35/644A61K47/26
Inventor 陈金林陈金庚梅曙明
Owner 射阳县庆缘康蜂产品厂
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com