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Active combined product extracted from leechee pulp, preparation method thereof and application thereof

A technology of lychee pulp and composition, which is applied in the development of health care products and medicine, can solve problems such as no anti-senile dementia treatment, and achieves the effects of low process cost, low price, simple process operation method, and inhibitory activity.

Inactive Publication Date: 2012-11-14
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there is no research on the effect of extracting active components from lychee pulp and applying them in the treatment of anti-senile dementia

Method used

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  • Active combined product extracted from leechee pulp, preparation method thereof and application thereof
  • Active combined product extracted from leechee pulp, preparation method thereof and application thereof
  • Active combined product extracted from leechee pulp, preparation method thereof and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Process for extracting the active composition from the pulp of lychees of the variety Blackleaf:

[0036] Pick the fresh lychee fruit of Huaizhi, peel the skin, remove the core, and mash the pulp. Add 6 times the volume of 50% ethanol solution to the crushed pulp, adjust the pH value to 3, place it in an environment of 40°C for 20 hours, filter and centrifuge to obtain the extract, concentrate it under reduced pressure (the temperature is 40°C) ℃) to obtain the crude extract of litchi pulp. After the crude extract was freeze-dried, it was dissolved in an appropriate amount of water, passed through a macroporous adsorption resin column (Amberlite XAD 16), eluted with a certain volume of water, and then eluted with 95% ethanol solution. The eluate from ethanol solution was concentrated under reduced pressure to obtain the active composition of litchi pulp.

Embodiment 2

[0038] 1. Effects of Active Compositions Extracted from Lychee (Black Leaf) Pulp on ?-Amyloid (A?) Aggregation

[0039] 1.1 Experimental method: Establish a pathological model of senile dementia (ie: ?-amyloid), observe the effect of the extracted active composition on ?-amyloid aggregation by in vitro incubation, and evaluate the extracted active composition with curcumin as a positive control Effects of substances on β-amyloid aggregation.

[0040] (1) Solution preparation:

[0041] Thioflavin T solution: Dissolve 3 mg of powder in glycine-NaOH buffer solution (50 mM, pH 8.5), and the concentration of the stock solution is 1 mM. The working concentration was diluted to 5 μM;

[0042] β-amyloid protein solution: β-amyloid protein powder is prepared into a 1500μM solution with phosphate buffer PBS (0.1M, pH 7.4), and stored in -80°C in aliquots, melted and diluted when used;

[0043] Extracted active composition solution: dissolved in dimethyl sulfoxide (DMSO, dimethylsulf...

Embodiment 3

[0069] Method for determination of polyphenol content in crude extract of litchi pulp (taking black leaves as an example):

[0070] 1. Determination of Total Polyphenol Content

[0071] 1.1 Drawing of the standard curve: the concentration of gallic acid is 500μg / ml (methanol solution), and then the concentration is: 0ug / ml, 25ug / ml, 50ug / ml, 100ug / ml, 200ug / ml, 400ug / ml, For a concentration of 500ug / ml, take 50ul and add 1ml of double distilled water and 0.5ml of Folin’s phenol reagent respectively, shake well, add 2.5ml of 20% sodium carbonate solution after 6min, react at room temperature for 30min (protect from light), at 765nm wavelength Measure its A value (using a full-wavelength microplate reader). With the concentration as the abscissa and the absorbance (deducting the blank) as the ordinate, draw a standard curve (see Figure 5 ).

[0072]1.2 Determination of the total polyphenol content in litchi pulp extract (composition contained in 95% eluent after separation b...

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Abstract

The invention discloses an active combined product extracted from leechee pulp, a preparation method thereof and an application thereof. The preparation method of the active combined product comprises the steps of: leaching the leechee pulp for 20h by 50% alcohol to obtain a leechee pulp coarse extract under the conditions that the PH value is 3 and the extraction temperature is 40 DEG C; and separating the coarse extract by macroporous absorption resin to obtain the leechee pulp active combined product. The leechee pulp active combined product is used for preparing the medicine for curing the senile dementia, can be used for effectively preventing the beta-amyloid from being gathered, is lower in cytotoxicity, and can be used for preparing the health product for developing the senile dementia. The leechee pulp active combined product has the characteristics of being good in anti-senile dementia effect, simple in technical operation method, high in industrial applicability, and low intechnical cost and price.

Description

technical field [0001] The invention relates to the technical field of medicine and the field of health care product development, in particular to a preparation method of an active composition of litchi pulp with an anti-senile dementia effect and its medical use and health care use. Background technique [0002] Alzheimer's disease (Alzheimer's disease) is a progressive neurodegenerative disease accompanied by cognitive, behavioral and functional disorders. It is the most common form of senile dementia. Its clinical symptoms include: Cognitive impairment, memory impairment, temporal and spatial orientation impairment, mental behavioral impairment, etc. Alzheimer's disease is a neurodegenerative disease caused by a variety of reasons, involving various pathological mechanisms and various pathological manifestations. The most important pathological manifestation is the appearance of amyloid protein (β-amyloid protein, Aβ) Aggregate formation of senile plaques (senile plaque,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/352A61K36/77A61P25/28A61K131/00
Inventor 欧田苗顾琼姚佩芬陈厚彬古练权黄志纾
Owner SUN YAT SEN UNIV
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