Streptomyces coelicolor trehalose synthase enzyme gene and application thereof

A technology of trehalose synthase and Streptomyces coelicolor, which is applied in the fields of genetic engineering and modern enzyme engineering, can solve the problems of different synthetic pathways, different enzymatic characteristics and enzyme activities, and different sources of strains, and achieve market competitiveness Effect

Inactive Publication Date: 2011-08-17
GUANGXI UNIV
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Problems solved by technology

[0004] At present, many literatures and patents have reported the cloning and isolation of genes related to trehalose synthesis, and the use of related enzymes of these trehalose synthase genes to prepare trehalose, but the synthesis pathways of these enzymes related to tr

Method used

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  • Streptomyces coelicolor trehalose synthase enzyme gene and application thereof
  • Streptomyces coelicolor trehalose synthase enzyme gene and application thereof
  • Streptomyces coelicolor trehalose synthase enzyme gene and application thereof

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Embodiment Construction

[0028] The technical solution of the present invention will be further described below through examples.

[0029] 1. Culture of Streptomyces coelicolor

[0030] Streptomyces coelicolor (Streptomyces coelicolor, strain number AS 4.1061) was purchased from the China Microorganism Collection Center and inoculated in the following medium (g / L): 4.0 g of glucose, 10 g of yeast extract, and 10 g of malt extract. The pH was adjusted to 7.2 with KOH, and then shaken on a constant temperature shaker at 25-30°C for 48 hours for the extraction of total DNA.

[0031] 2. Cloning, expression and crude enzyme preparation of Streptomyces coelicolor trehalose synthase gene

[0032] According to the Streptomyces coelicolor gene sequence published in Genbank, the sequence annotated as a possible trehalose synthase gene was named Sct, corresponding primers were designed to amplify the gene and connected to the expression vector, and then introduced into Escherichia coli for expression. The prime...

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Abstract

The invention relates to a streptomyces coelicolor trehalose synthase enzyme gene. The gene is cloned from streptomyces coelicolor (Streptomyces coelicolor, AS 4.1061). A trehalose synthase enzyme expressed by the gene can be used for converting maltose into trehalose under the normal biochemical reaction conditions and has the characteristics of high reaction speed, high conversion rate of a substrate and few byproducts. The content of trehalose in a product generated in the process of converting the maltose at a temperature of 25 DEG C can achieve over 75 percent and only little glucose is generated. The streptomyces coelicolor trehalose synthase enzyme gene is beneficial for improving the conversion rate of the substrate and reducing the production cost. The gene can be applied to production of the trehalose. The trehalose can be applied to the fields of food industry, beauty cosmetics, medical biological products and the like.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering and modern enzyme engineering, and specifically relates to a trehalose synthase gene of Streptomyces coelicolor and its application. Background technique [0002] Trehalose (Trehalose) is a non-reducing disaccharide formed by connecting two molecules of glucose with α, α-1, 1-glycosidic bonds. It was first extracted from ergot bacteria in rye by Wiggers in 1832, and then Recent studies have found that trehalose widely exists in microorganisms, shrimp, Saccharomyces cerevisiae, mushrooms and various fungi, insects, plants, etc. Exogenous trehalose also has a good non-specific protective effect on organisms, biological macromolecules, and biofilms. It can protect cells from damage caused by environmental changes, such as dehydration and hypertonic changes. Effective protection of biofilm, protein and DNA, etc., so it is known as "sugar of life". The content of trehalose in nature is ve...

Claims

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Application Information

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IPC IPC(8): C12N15/52C12N9/00C12P19/12
Inventor 韦宇拓黄日波梁甲元杜丽琴
Owner GUANGXI UNIV
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