Thermomonospora curvata mycose synthetase gene and application thereof

Abstract

The invention discloses a thermomonospora curvata mycose synthetase gene and application thereof. The gene is cloned from thermomonospora curvata. Mycose synthetase expressed by the gene has two different transformation functions. The mycose synthetase can be used for transforming maltose into mycose and transforming cane sugar into kelp ketose under normal biochemical reaction conditions. The gene can be applied to the production of mycose by transforming maltose, and can be used for transforming cane sugar into kelp ketose. The mycose and the kelp ketose can be applied to the fields of food industry, beverages, functional foods, beautifying cosmetics, medical biological products and the like.

Description

Technical field [0001] The invention belongs to the technical fields of genetic engineering and modern enzyme engineering, and specifically relates to a trehalose synthase gene of Thermomonas flexus and its application. Background technique [0002] Trehalose is a non-reducing disaccharide composed of two molecules of glucose linked by α,α-1,1-glycosidic bonds. It was first extracted from the ergot of rye by Wiggers in 1832, and then The research found that trehalose is widely present in microorganisms, shrimps, Saccharomyces cerevisiae, mushrooms and various fungi, insects, plants, etc. Exogenous trehalose also has a good non-specific protective effect on organisms, biological macromolecules, and biological membranes. It can protect cells from damage caused by environmental changes, such as dehydration and hypertonic changes. The effective protection of biological membranes, proteins and DNA, etc., is therefore known as the "sugar of life". The content of trehalose in nature i...

AI Technical Summary

Problems solved by technology

There are currently three types of enzymes that can convert sucrose into trehalulose, which are publicly reported in the literature. One is sucrose isomerase, also known as α-glucosyltransferase. The conversion rate of sucrose can reach 90%, but its conversion rate of sucrose is The product is a mixed syrup of trehalulose and isomaltulose in equal proportions; the second is trehalulose ketose synthase, which can convert sucrose into trehalulose, and the conversion rate of sucrose can reach more than 70%, but the general reaction speed is slower Slow, but this type of enzyme can not use maltose as a substrate to generate trehalose; the third type that can convert sucrose to generate trehalulose is trehalose synthase. Some studies have found that some trehalose synthases can use sucrose as a substrate to generate trehalone However, it is currently reported that the trehalose synthase that can convert sucrose into trehalone takes sucrose as the substrate, and the conversion rate of sugar sucrose is low, generally less than 5%.

[0006] At present, many literatures and patents have reported the cloning and isolation of genes related to trehalose synthesis, and the use of related enzymes of these trehalose synthase genes to prepare trehalose, but the synthesis pathways of these enzymes related to trehalose synthesis are different, or bacteria The different sources lead to the great difference in the DNA sequence and amino acid sequence, and the enzymatic properties and enzyme activities are different, and most of them have not been applied on an industrial scale.

There is no relevant report about trehalulose synthase gene in China, and there is no relevant report about the application of trehalulose synthase and the patent of trehalulose production.

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