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Method for measuring low density lipoprotein cholesterol

A low-density lipoprotein, high-density lipoprotein technology, applied in the direction of measuring devices, biochemical equipment and methods, biological testing, etc.

Inactive Publication Date: 2011-08-24
FUJIFILM CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the method described in this document has difficulties in solving the problems that it requires incubation for 15 minutes or longer, and that it requires two types of reagents

Method used

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  • Method for measuring low density lipoprotein cholesterol
  • Method for measuring low density lipoprotein cholesterol
  • Method for measuring low density lipoprotein cholesterol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] (1) Preparation of polyamine aqueous solution

[0080] Using poly(allylamine) PAA-05 (Nitto Boseki Co., Ltd) or diallylamine hydrochloride-sulfur dioxide copolymer PAS-92 (Nitto Boseki Co., Ltd), with 0.1M MOPS buffer (pH 7.0 ) to prepare 2% polyamine aqueous solution.

[0081] (2) Preparation of lipoprotein solution

[0082] Low-density lipoprotein LDL (Athens Research & Technology, Inc), very low-density lipoprotein VLDL (Athens Research & Technology, Inc), high-density lipoprotein (Athens Research & Technology, Inc), human serum albumin HSA (Sigma -Aldrich) and human serum globulin HSG (Sigma-Aldrich) to prepare the following lipoprotein solutions.

[0083] A mixed solution of 150mg / dL LDL-C and 60mg / dL VLDL-C (containing 5% HSA and 2% HSG)

[0084] Mixed solution of 150mg / dL LDL-C and 100mg / dL HDL-C (containing 5% HSA and 2% HSG)

[0085] (3) Detection by agarose gel electrophoresis

[0086] 100 µl of the 2% polyamine aqueous solution prepared in (1) above was ...

Embodiment 2

[0104] (1) Preparation of lipoprotein solution

[0105] Using low-density lipoprotein LDL (Athens Research & Technology, Inc), very low-density lipoprotein VLDL (Athens Research & Technology, Inc), high-density lipoprotein (Athens Research & Technology, Inc), human serum albumin HSA (Sigma -Aldrich) and human serum globulin HSG (Sigma-Aldrich) to prepare the following lipoprotein solutions.

[0106] 50mg / dL LDL-C solution (containing 5% HSA and 2% HSG)

[0107] 75mg / dL LDL-C solution (containing 5% HSA and 2% HSG)

[0108] 150mg / dL LDL-C solution (containing 5% HSA and 2% HSG)

[0109] 250mg / dL LDL-C solution (containing 5% HSA and 2% HSG)

[0110] A mixed solution of 75mg / dL LDL-C and 30mg / dL VLDL-C (containing 5% HSA and 2% HSG)

[0111] Mixed solution of 75mg / dL LDL-C and 60mg / dL VLDL-C (containing 5% HSA and 2% HSG)

[0112] Mixed solution of 150mg / dL LDL-C and 50mg / dL HDL-C (containing 5% HSA and 2% HSG)

[0113] Mixed solution of 150mg / dL LDL-C and 100mg / dL HDL-C (...

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Abstract

The invention provides a method for measuring low density lipoprotein cholesterol. It is an object of the present invention to provide a method capable of measuring low density lipoprotein cholesterol (LDL) in a body fluid with high selectivity. The present invention provides a method for measuring low density lipoprotein cholesterol (LDL-C) in a body fluid, which comprises treating the body fluid with a polymer compound having an allylamine or diallylamine unit, and measuring the low density lipoprotein cholesterol (LDL-C) using (a) cholesterol esterase and (b) cholesterol oxidase or cholesterol dehydrogenase.

Description

technical field [0001] The present invention relates to a method for measuring low-density lipoprotein cholesterol (LDL-C) in a sample, wherein a polymer compound having an allylamine or diallylamine unit specifically forms a complex with non-LDL (VLDL or HDL). Background technique [0002] In addition to free fatty acids bound to albumin, lipids present in the blood are incorporated into the structure of lipoproteins, and they form as chylomicrons (CHM), very low-density lipoprotein (VLDL), low-density lipoprotein (LDL), It exists in the form of high-density lipoprotein (HDL), among others. Cholesterol is particularly distributed in VLDL, LDL and HDL. LDL is known to function to transport cholesterol, and high levels of LDL promote arteriosclerosis. It is known that measurement of low-density lipoprotein cholesterol (LDL-C) in blood becomes an indicator of occurrence and progression of arteriosclerosis. [0003] Burstein M et al. (Rapid Method for the Isolation of Lipopr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/60
CPCG01N33/92C12Q1/26C12Q1/44C12Q1/60Y10T436/25375
Inventor 猪股弘子M·朱马维德
Owner FUJIFILM CORP