Multienzyme complex preparation and preparation method and application thereof

Abstract

The invention provides a multienzyme complex preparation and a preparation method and application thereof. The multienzyme complex preparation is characterized by being prepared by adding water to multienzyme complex, wherein the weight ratio of the multienzyme complex to the water is 1:(50-100); and the multienzyme complex comprises the following components in parts by weight: 30-35 parts of laccase, 25-35 parts of xylanase, 10-20 parts of pectinase, 0.1-10 parts of amylase, 0.3-10 parts of lipase, 0.1-5 parts of tannase, 0.1-3 parts of coenzyme, 0.1-3 parts of stabilizing agent and 0.3-25 parts of diatomite. The multienzyme complex preparation can be applied to the preparation process of the fiber pulp for papermaking, the fiber pulp in accordance with the papermaking production requirement can be obtained without further adopting the high-temperature / pressure chemical cooking method in the pulping process, and the original lignin can be preserved to the greatest extent in the fiber pulp preparing process.

Description

technical field

[0001] The invention relates to a biological preparation and its preparation method and application, in particular to a multi-enzyme compound preparation and its preparation method and application. Background technique

[0002] In recent years, more and more biological agents have been widely used in the field of industrial production, which greatly reduces the pollution to the environment while replacing chemical agents. For example, in the field of paper industry production, the chemical pulping method is pretreated with enzyme Instead of the pure chemical pulping method, the use of chemicals in the pure chemical pulping method is greatly reduced, and the discharge of sewage with extremely high BOD and COD loads and highly toxic and carcinogenic substances in the pulping process is greatly reduced.

[0003] However, the existing enzyme pretreatment chemical pulping method still needs to use high temperature and high pressure chemical cooking (mainly alkali ...

Examples

Embodiment 1

[0039] Embodiment 1, utilize multi-enzyme compound preparation of the present invention to obtain fiber slurry from rice straw

[0040] For the straw of 100kg, the multi-enzyme compound preparation used in the present embodiment is prepared by adding 35-70kg of water to the multi-enzyme compound of 0.7kg, and the formula of the multi-enzyme compound includes the following components by weight: 31 - 33 parts of laccase; 25-26 parts of xylanase; 10-15 parts of pectinase; 1-5 parts of amylase; 6-8 parts of lipase; 3-5 parts of tannase The coenzyme of 1-3 parts; The stabilizer of 2-3 parts; The diatomite of 3-5 parts; Described stabilizer is made up of stabilizer A, stabilizer B and stabilizer C; Described stabilizer A accounts for stable 30% of the dosage formula consists of Ca 2+ and polyvinylpyrrolidone; the stabilizer B accounts for 50% of the stabilizer formula, consisting of EDTA and dithiothreitol; the stabilizer C accounts for 20% of the stabilizer formula, composed of po...

Embodiment 2

[0054] Embodiment 2, using the multi-enzyme compound preparation of the present invention to obtain fiber slurry from corn stalks

[0055] For 100 kg of corn stalks, the multi-enzyme compound preparation used in this example is prepared by adding 45-65 kg of water to 1 kg of the multi-enzyme compound. The formula of the multi-enzyme compound includes the following components by weight: 30 - 31 parts of laccase; 27-28 parts of xylanase; 15-20 parts of pectinase; 3-5 parts of amylase; 0.5-3 parts of lipase; 0.1-1 part of tannase The coenzyme of 0.5-1 part; The stabilizer of 1-3 part; The diatomite of 10-15 part; Described stabilizer is made up of stabilizer A, stabilizer B and stabilizer C; Described stabilizer A accounts for stable 30% of the dosage formula consists of Ca 2+ and polyvinylpyrrolidone; the stabilizer B accounts for 50% of the stabilizer formula, consisting of EDTA and dithiothreitol; the stabilizer C accounts for 20% of the stabilizer formula, composed of polyvi...

Embodiment 3

[0071] Embodiment 3, utilize multi-enzyme compound preparation of the present invention to obtain fiber slurry from wheat straw

[0072] For 100kg of wheat straw, the multi-enzyme compound preparation used in this embodiment is prepared by adding 43-50 kg of water to the multi-enzyme compound of 0.85 kg, and the formula of the multi-enzyme compound includes the following components by weight: 33 25-26 parts of xylanase; 10-11 parts of pectinase; 1-3 parts of amylase; 8-9 parts of lipase; 3-5 parts of tannase; 0.1 -1 part of coenzyme; 1-3 parts of stabilizer; 3-10 parts of diatomaceous earth; the stabilizer is composed of stabilizer A, stabilizer B and stabilizer C; the stabilizer A accounts for the stabilizer formula 30% of the amount consists of Ca 2+ and polyvinylpyrrolidone; the stabilizer B accounts for 50% of the stabilizer formula, consisting of EDTA and dithiothreitol; the stabilizer C accounts for 20% of the stabilizer formula, composed of polyvinylpyrrolidone and EDT...

technical field

[0001] The invention relates to a biological preparation and its preparation method and application, in particular to a multi-enzyme compound preparation and its preparation method and application. Background technique

[0002] In recent years, more and more biological agents have been widely used in the field of industrial production, which greatly reduces the pollution to the environment while replacing chemical agents. For example, in the field of paper industry production, the chemical pulping method is pretreated with enzyme Instead of the pure chemical pulping method, the use of chemicals in the pure chemical pulping method is greatly reduced, and the discharge of sewage with extremely high BOD and COD loads and highly toxic and carcinogenic substances in the pulping process is greatly reduced.

[0003] However, the existing enzyme pretreatment chemical pulping method still needs to use high temperature and high pressure chemical cooking (mainly alkali ...

Examples

Embodiment 1

[0039] Embodiment 1, utilize multi-enzyme compound preparation of the present invention to obtain fiber slurry from rice straw

[0040] For the straw of 100kg, the multi-enzyme compound preparation used in the present embodiment is prepared by adding 35-70kg of water to the multi-enzyme compound of 0.7kg, and the formula of the multi-enzyme compound includes the following components by weight: 31 - 33 parts of laccase; 25-26 parts of xylanase; 10-15 parts of pectinase; 1-5 parts of amylase; 6-8 parts of lipase; 3-5 parts of tannase The coenzyme of 1-3 parts; The stabilizer of 2-3 parts; The diatomite of 3-5 parts; Described stabilizer is made up of stabilizer A, stabilizer B and stabilizer C; Described stabilizer A accounts for stable 30% of the dosage formula consists of Ca 2+ and polyvinylpyrrolidone; the stabilizer B accounts for 50% of the stabilizer formula, consisting of EDTA and dithiothreitol; the stabilizer C accounts for 20% of the stabilizer formula, composed of po...

Embodiment 2

[0054] Embodiment 2, using the multi-enzyme compound preparation of the present invention to obtain fiber slurry from corn stalks

[0055] For 100 kg of corn stalks, the multi-enzyme compound preparation used in this example is prepared by adding 45-65 kg of water to 1 kg of the multi-enzyme compound. The formula of the multi-enzyme compound includes the following components by weight: 30 - 31 parts of laccase; 27-28 parts of xylanase; 15-20 parts of pectinase; 3-5 parts of amylase; 0.5-3 parts of lipase; 0.1-1 part of tannase The coenzyme of 0.5-1 part; The stabilizer of 1-3 part; The diatomite of 10-15 part; Described stabilizer is made up of stabilizer A, stabilizer B and stabilizer C; Described stabilizer A accounts for stable 30% of the dosage formula consists of Ca 2+ and polyvinylpyrrolidone; the stabilizer B accounts for 50% of the stabilizer formula, consisting of EDTA and dithiothreitol; the stabilizer C accounts for 20% of the stabilizer formula, composed of polyvi...

Embodiment 3

[0071] Embodiment 3, utilize multi-enzyme compound preparation of the present invention to obtain fiber slurry from wheat straw

[0072] For 100kg of wheat straw, the multi-enzyme compound preparation used in this embodiment is prepared by adding 43-50 kg of water to the multi-enzyme compound of 0.85 kg, and the formula of the multi-enzyme compound includes the following components by weight: 33 25-26 parts of xylanase; 10-11 parts of pectinase; 1-3 parts of amylase; 8-9 parts of lipase; 3-5 parts of tannase; 0.1 -1 part of coenzyme; 1-3 parts of stabilizer; 3-10 parts of diatomaceous earth; the stabilizer is composed of stabilizer A, stabilizer B and stabilizer C; the stabilizer A accounts for the stabilizer formula 30% of the amount consists of Ca 2+ and polyvinylpyrrolidone; the stabilizer B accounts for 50% of the stabilizer formula, consisting of EDTA and dithiothreitol; the stabilizer C accounts for 20% of the stabilizer formula, composed of polyvinylpyrrolidone and EDT...