Method for creating new peanut specie
A new germplasm, peanut technology, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of difficult selection, low beneficial mutation rate, and high mutagenesis cost, and achieve cost reduction and improvement. Beneficial Variation Rate, Social Benefit, and Economic Benefit Significant Effects
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Embodiment 1
[0018] After the Haihua No. 1 peanuts mature, 100 plump seeds are selected, sterilized with 0.05% HgCl for 30 minutes, sterilized on the surface, rinsed with sterile water for 3 times, the seed coat and two cotyledons are removed to obtain peanut embryos. The peanut embryos were irradiated with 60Co-γ rays at a dose of 3000 roentgens for mutagenesis. The mutagenized embryos were inoculated in MSB supplemented with 1.5mg / L NAA and 5mg / L BAP 5 Calli were induced on medium and transferred to MSB supplemented with 3mg / L BAP after 20 days 5 Tissue culture regenerated shoots were obtained on shoot differentiation medium. When the regenerated seedling grows to a seedling height of 3-4 cm, cut the tissue-cultured regenerated seedling from the base of the bud cluster, and cut the lower end of the scion (the tissue-cultured regenerated seedling after embryo mutagenesis)) to a length of about 0.5-1 cm on the ultra-clean platform. 60° V-shaped wound with smooth incision. The rootstock ...
Embodiment 2
[0020] After the Huayu No. 20 peanuts matured, 200 plump seeds were selected, sterilized with 0.05% HgCl for 30 minutes, sterilized on the surface, rinsed with sterile water 3 times, removed the seed coat and two cotyledons to obtain peanut embryos. The peanut embryos were irradiated with 60Co-γ rays at a dose of 5000 roentgens. The mutagenized embryos were inoculated in MSB supplemented with 1.5mg / L NAA and 5mg / LBAP 5 Calli were induced on medium and transferred to MSB supplemented with 3mg / L BAP after 20 days 5 Tissue culture regenerated shoots were obtained on shoot differentiation medium. When the tissue-cultured regenerated seedlings grow to 3-4 cm in height, cut the tissue-cultured regenerated seedlings from the base of the bud cluster, cut the lower end of the scion into a 60° V-shaped wound with a length of about 0.5-1 cm on the ultra-clean platform, and the incision is smooth. The rootstock is the unmutated seedling of Haihua No. 1, with a seedling height of 5-7 cm....
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