Method for creating new peanut specie

A new germplasm, peanut technology, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of difficult selection, low beneficial mutation rate, and high mutagenesis cost, and achieve cost reduction and improvement. Beneficial Variation Rate, Social Benefit, and Economic Benefit Significant Effects

Inactive Publication Date: 2011-11-23
杨庆利
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  • Summary
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  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0004] The problem to be solved by the present invention is to provide a method for creating new germplasm through radiation mutagenesis, so as

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] After the Haihua No. 1 peanuts mature, 100 plump seeds are selected, sterilized with 0.05% HgCl for 30 minutes, sterilized on the surface, rinsed with sterile water for 3 times, the seed coat and two cotyledons are removed to obtain peanut embryos. The peanut embryos were irradiated with 60Co-γ rays at a dose of 3000 roentgens for mutagenesis. The mutagenized embryos were inoculated in MSB supplemented with 1.5mg / L NAA and 5mg / L BAP 5 Calli were induced on medium and transferred to MSB supplemented with 3mg / L BAP after 20 days 5 Tissue culture regenerated shoots were obtained on shoot differentiation medium. When the regenerated seedling grows to a seedling height of 3-4 cm, cut the tissue-cultured regenerated seedling from the base of the bud cluster, and cut the lower end of the scion (the tissue-cultured regenerated seedling after embryo mutagenesis)) to a length of about 0.5-1 cm on the ultra-clean platform. 60° V-shaped wound with smooth incision. The rootstock ...

Embodiment 2

[0020] After the Huayu No. 20 peanuts matured, 200 plump seeds were selected, sterilized with 0.05% HgCl for 30 minutes, sterilized on the surface, rinsed with sterile water 3 times, removed the seed coat and two cotyledons to obtain peanut embryos. The peanut embryos were irradiated with 60Co-γ rays at a dose of 5000 roentgens. The mutagenized embryos were inoculated in MSB supplemented with 1.5mg / L NAA and 5mg / LBAP 5 Calli were induced on medium and transferred to MSB supplemented with 3mg / L BAP after 20 days 5 Tissue culture regenerated shoots were obtained on shoot differentiation medium. When the tissue-cultured regenerated seedlings grow to 3-4 cm in height, cut the tissue-cultured regenerated seedlings from the base of the bud cluster, cut the lower end of the scion into a 60° V-shaped wound with a length of about 0.5-1 cm on the ultra-clean platform, and the incision is smooth. The rootstock is the unmutated seedling of Haihua No. 1, with a seedling height of 5-7 cm....

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PUM

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Abstract

The invention relates to a method for creating a new peanut specie, belonging to the technical field of the peanut genetic breeding method. The method adopts radiation mutation to create a new specie. The method comprises the following steps: the embryo of a mature seed is used as the radiation material; after the mutation, the mutated embryo is used to perform tissue culture and obtain a tissue culture regeneration seedling, and the tissue culture regeneration seedling is grafted in a field by using a seedling as rootstock. The beneficial mutation rate of the new specie obtained by the method is up to more than 30%. By using the method, the problem of the traditional method that the beneficial new peanut specie is difficult to create is solved, and the method has remarkable social and economic benefits.

Description

technical field [0001] The invention relates to a method for creating new germplasm of peanuts, and belongs to the technical field of peanut genetic breeding methods. Background technique [0002] Peanut is an important oil crop, cash crop and edible crop in China. my country is the world's largest peanut producer, consumer and exporter. my country's peanut industry is adjusting the agricultural industrial structure, increasing farmers' income, earning foreign exchange through export, and ensuring national oil supply. made an important contribution. However, since the 1980s, there has been no fundamental change in the yield and quality of peanuts in my country, and the potential for increasing the production of peanut varieties has not been effectively improved. Many high-yield records of peanuts in my country are the main variety Haihua 1 in the 1980s. number created. The oil content has not exceeded the new peanut variety Xuhua No. 9 bred in 2003. Therefore, on the basis ...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G1/06
Inventor 杨庆利禹山林王晶珊
Owner 杨庆利
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