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Method for preparing self-assembled medicine-carried microspheres by combining high-voltage electrostatic liquid droplet method and layer-by-layer self-assembly method

A technology of layer-by-layer self-assembly and drug-loaded microspheres, which is applied in the direction of pharmaceutical formulations, medical preparations containing active ingredients, and devices that make drugs into special physical or ingestible forms. It can solve drug burst release, protein drug Reduced stability and other issues, to achieve the effect of protective activity, good uniformity, and avoid burst release

Active Publication Date: 2013-02-20
薛巍
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Problems solved by technology

[0002] Growth factors are a class of bioactive polypeptides or proteins that can stimulate target cells to produce certain biological effects. At present, the main packaging method of such protein drugs is to use polymer microspheres prepared by organic solvents such as chloroform and methylene chloride. The problem of decreased stability of protein drugs, and problems such as sudden release of drugs

Method used

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  • Method for preparing self-assembled medicine-carried microspheres by combining high-voltage electrostatic liquid droplet method and layer-by-layer self-assembly method
  • Method for preparing self-assembled medicine-carried microspheres by combining high-voltage electrostatic liquid droplet method and layer-by-layer self-assembly method
  • Method for preparing self-assembled medicine-carried microspheres by combining high-voltage electrostatic liquid droplet method and layer-by-layer self-assembly method

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Experimental program
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Effect test

Embodiment

[0025] Experimental Materials:

[0026] Sodium alginate (viscosity of 2% aqueous solution at 25°C is 250cps), Sigma company; sodium dextran sulfate (Mw > 5000 000), Sigma company; human basic fibroblast growth factor, Bioengineering Institute of Jinan University; chitosan Sugar (viscosity 40~100MPa.s), Guangzhou Qiyun Biotechnology Co., Ltd.; human basic ELISA kit, Dakowei Biotechnology Co., Ltd.; disodium edetate, Guangzhou Chemical Reagent Factory; calcium chloride (analytical pure), Guangzhou Chemical Reagent Factory; absolute ethanol (analytical pure), Tianda Chemical Reagent Factory, Dongli District, Tianjin; experimental water was Milli-Q (Integral 5) deionized water.

[0027] Continuous zoom stereo microscope (Smz-T4), Chongqing Auto Optical Instrument Company; scanning electron microscope (XL-30), Philips Company; high voltage DC power supply (DW-P503-4ACCD), Tianjin Sanchuan Company; micro syringe pump ( LSP01-1A), Baoding Lange Company; digital display air bath cons...

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Abstract

The invention discloses a method for preparing self-assembled medicine-carried microspheres by combining a high-voltage electrostatic liquid droplet method and a layer-by-layer self-assembly method. In the invention, growth factor-containing sodium alginate aqueous solution is used as raw material solution; calcium chloride solution is used as a gel bath; microcapsules are prepared in a high-voltage electrostatic field; growth factor is uniformly distributed in microgel; and then a plurality of high molecular polyelectrolyte membranes are assembled on the surface of the microgel by using an electrostatic nano layer-by-layer self-assembly technology to obtain the self-assembled medicine-carried microspheres. The self-assembled medicine-carried microspheres prepared by the method have smooth and round surfaces and high uniformity; the activity of the growth factor can be protected; burst release can be effectively avoided; and long-acting constant-speed release is realized.

Description

technical field [0001] The invention relates to the field of microsphere preparation methods, in particular to a method for preparing self-assembled drug-loaded microspheres by combining a high-voltage electrostatic droplet method and a layer-by-layer self-assembly method. Background technique [0002] Growth factors are a class of bioactive polypeptides or proteins that can stimulate target cells to produce certain biological effects. At present, the main packaging method of such protein drugs is to use polymer microspheres prepared by organic solvents such as chloroform and methylene chloride. The problem of decreased stability of protein drugs, and problems such as sudden release of drugs will also occur. How to use relatively mild conditions to encapsulate protein drugs to maintain their long-term activity and achieve controlled release is an important direction in the current research on drug carrier systems. Contents of the invention [0003] The purpose...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/16A61K38/18A61K47/36A61J3/00
Inventor 薛巍何留民左琴华谢莎莎黄跃新施云峰张奕
Owner 薛巍
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