A method for semi-continuous fermentation to produce bio-butanol

A bio-butanol, semi-continuous technology, applied in the field of bioengineering, to achieve the effects of shortening the production cycle, high solvent production intensity, and high solvent output

Active Publication Date: 2011-12-21
江苏惠利生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] At present, there is no industrial report on the semi-continuous fermentation of sugar raw material m

Method used

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  • A method for semi-continuous fermentation to produce bio-butanol
  • A method for semi-continuous fermentation to produce bio-butanol
  • A method for semi-continuous fermentation to produce bio-butanol

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Embodiment 1

[0029] Clostridium saccharobutylicum Clostridium saccharobutylicumDSM 13864 is a strain for the production of bio-butanol by fermentation, using sugarcane molasses as the fermentation raw material.

[0030] The seed medium is RCM medium ( / L): yeast extract 3g, beef extract 10g, peptone 10g, soluble starch 1g, glucose 5g, cysteine ​​hydrochloride 0.5g, NaCl 3g, NaAC 3g, resazurin 3mg , pH 6.5. Transfer 1 mL of strain spore liquid to 10 mL test tube seed medium, place in a vacuum desiccator to evacuate to a pressure of 0.065 MPa, and incubate at 37°C for 12-18 h.

[0031] Primary feed medium (SSM1) ( / L): total sugar 2%, CaCO 3 3.2 g, (NH 4 ) 2 SO 4 2 g, K 2 HPO 4 0.5 g, corn steep liquor dry powder 20g, MnSO 4 ·H 2 O 0.01 g, pH 6.7.

[0032] Secondary feed medium (SSM2) ( / L): total sugar 8%, CaCO 3 3.2 g, (NH 4 ) 2 SO 4 2 g, K 2 HPO 4 0.5 g, corn steep liquor dry powder 10g, MnSO 4 ·H 2 O 0.01g, pH 6.4, sterilized at 121°C for 20 min.

[0033] The prim...

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Abstract

The invention relates to a method for producing biological butyl alcohol by semicontinuous fermentation, belonging to the technical field of biological engineering. The invention relates to a semicontinuous fermentation method, particularly application of Clostridium saccharobutyricum DSM13864 in a biological butyl alcohol preparation process by fermenting cane molasses, beet molasses, lignocellulose hydrolyzed syrup and other saccharide raw materials. By using the method provided by the invention, higher solvent yield and solvent production intensity can be obtained; the invention only needssterilization and inoculation once, thereby saving the non-fermentation time, including repeated tank washing, sterilization and the like, shortening the production cycle and greatly increasing the equipment utilization ratio; and the fermentation plant is simple, easy to implement automatic control, and applicable to industrialization.

Description

technical field [0001] The invention provides a method for producing bio-butanol by semi-continuous fermentation, which belongs to the technical field of bioengineering. Clostridium saccharobutyricum Clostridium saccharobutylicum DSM 13864 Application in the production of bio-butanol from sugary raw materials such as fermented sugarcane molasses, beet molasses and lignocellulose hydrolysis syrup. Background technique [0002] Butanol is customarily referred to as butanol, and its chemical formula is C 4 h 10 O, a colorless transparent liquid with a strong odor, slightly soluble in water, poisonous and flammable, belonging to Class II flammable dangerous goods. Butanol is an important C4 platform compound and also a strategic product with a wide range of uses, such as n-butyl phthalate, fatty dibasic acid and butyl phosphate, butyl acrylate and butyl acetate, etc. Synthesis; it can be oxidized to produce butyraldehyde or butyric acid; it can also be used as an extraction ...

Claims

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Application Information

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IPC IPC(8): C12P7/16C12R1/145
Inventor 倪晔孙志浩王云
Owner 江苏惠利生物科技有限公司
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