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Temperature-pH dual-sensitivity gel microsphere as well as preparation method and application thereof

A technology of gel microspheres and ambidextrous, which is applied in the direction of immobilization on/in the organic carrier, which can solve the problems of cumbersome operation and inability to achieve synchronous immobilization.

Active Publication Date: 2013-06-05
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] Chinese patent (publication number CN1328067A) discloses a preparation method of porous temperature-sensitive poly(N-isopropylacrylamide) hydrogel, adding CaCO 3 As a porogen to prepare porous hydrogels, CaCO 3 The particle size is used to adjust the pore size of the gel, but after the preparation of this method, the porogen CaCO 3 Dissolve it with hydrochloric acid. Hydrochloric acid will damage the immobilized enzymes, drugs, cells, etc. in the gel. This method cannot achieve simultaneous immobilization, and the operation is cumbersome, and the preparation is a large gel.

Method used

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  • Temperature-pH dual-sensitivity gel microsphere as well as preparation method and application thereof
  • Temperature-pH dual-sensitivity gel microsphere as well as preparation method and application thereof
  • Temperature-pH dual-sensitivity gel microsphere as well as preparation method and application thereof

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preparation example Construction

[0039] In the preparation method of the present invention, the W / O emulsion is prepared by a microporous membrane emulsification method, the particle size of the gel microspheres can be adjusted by adjusting the pore size of the hydrophobic microporous membrane, and the particle size of the gel microspheres is uniform. The particle size distribution coefficient calculated by the following formula is not more than 20%:

[0040] C.V.={[∑(di-d)2 / N]1 / 2 / d}×100%

[0041] In the formula, C.V. represents the particle size distribution coefficient; di represents the diameter of each nano-microsphere; d represents the number average particle diameter of the microspheres, d=Σdi / N; N is the number of microspheres used to calculate the particle size, and N≥200 pieces.

[0042] Method provided by the invention comprises the steps:

[0043] 1) Provide an aqueous solution in which the water-soluble monomer N-isopropylacrylamide, acrylic acid, initiator, and crosslinking agent are dissolved as...

Embodiment 1

[0069]Add 10g of N-isopropylacrylamide into 100ml of n-hexane, heat it to 40°C to dissolve it, and then cool it down slowly. After crystallization, filter it with suction, put it in the refrigerator overnight, filter it the next day to get a filter cake, and recrystallize it with n-hexane for later use. A hydrophobic glass membrane was used as the microporous membrane. Accurately weigh 0.85g of refined N-isopropylacrylamide, 0.15g (15wt% of total monomers) acrylic acid, 0.05g (15wt% of total monomers) methylenebisacrylamide MBA in 8ml deionized water, and stir well. Then add 0.05g (3wt% of the total monomer amount) initiator APS, add deionized water to a total volume of 10ml, and magnetically stir to make it fully dissolved. Take 80ml of cyclohexane and 20ml of chloroform and mix evenly, add 5.0g (5% g / ml of oil phase mass) of emulsifier SPAN80, and stir magnetically to make it evenly mixed. Under the nitrogen pressure of 2.5KPa, the water phase is pressed into the oil phas...

Embodiment 2

[0071] Poly(N-isopropylacrylamide-co-acrylic acid) microspheres were prepared using the same device and method as in Example 1, the difference being that the total amount of N-isopropylacrylamide and acrylic acid was kept at 1 g, N-isopropylacrylamide The amount of propylacrylamide was changed to 0.95g (95% by weight of the total monomer), and the amount of acrylic acid was 0.05g (5% by weight of the total monomer), resulting in poly(N-isopropylacrylamide-co-acrylic acid) The average pore diameter of the microspheres is 27.04 nanometers, and the average particle diameter of the microspheres is 24.92 micrometers. The C.V. is 14.47%.

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Abstract

The invention provides a gel microsphere product having temperature-pH dual-sensitivity. The average pore size of the gel microsphere is in a range of nanometers to 200 nm and the average grain size thereof is 1-100 microns. The preparation method comprises the following steps of: (1) providing an aqueous solution which is dissolved with water-soluble monomer N-isopropyl acrylamide, acrylic acid,an initiator and a cross-linking agent as an aqueous phase W; (2) providing oily substance which is dissolved with an oil soluble emulsifying agent and is insoluble with water as an oil phase O; (3) mixing the aqueous phase with the oil phase to obtain a W / O emulsion, agitating and introducing an inert gas; (4) keeping the inert gas atmosphere, adding an accelerant into the W / O emulsion, and polymerizing to obtain the gel microsphere at a temperature which is lower than the critical dissolving temperature of N-isopropyl acrylamide; and (5) washing the polymerized gel microsphere to obtain thegel microsphere product. The invention further provides application of the gel microsphere.

Description

technical field [0001] The invention relates to the fields of functional polymer materials and biochemical separation and immobilized enzyme carriers. More specifically, it relates to a gel microsphere with temperature-pH dual sensitivity. [0002] The present invention also relates to a preparation method of the above-mentioned gel microspheres. [0003] The present invention also relates to the application of the above-mentioned gel microspheres. Background technique [0004] Smart hydrogel is composed of a hydrophilic three-dimensional cross-linked polymer network and a medium (water), and its physical and chemical properties and structure change with changes in the external environment (such as temperature, pH, optical field, magnetic field, etc.) Molecular aggregates are widely used in chemical, pharmaceutical, mechanical and other fields. Poly(N-isopropylacrylamide) (PNIPAM) is a typical temperature-sensitive polymer and one of the most commonly used materials for t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/08C12N11/04C08F220/54C08F220/06C08F2/32C08J9/26
Inventor 马光辉王玉霞司天宝苏志国
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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