Method for in vitro induction of human amniotic mesenchymal stem cells (hAMSCs) differentiated into insulin-secreting cells (ISCs)

Abstract

The invention discloses a method for the in vitro induction of hAMSCs differentiated into ISCs. According to the method, the extracted hAMSCs are cultured for 7 days by a serum-free high sugar-DMEM induction medium containing niacinamide of 10mmol / L and an N2 supplement. The hAMSCs induced by the method of the invention express insulin gene mRNA and PDX-1 gene mRNA, and the content of insulin in the supernatant of the culture reaches above 330muIU / ml; and the method of the invention allows the in vitro induction of the hAMSCs differentiated into the ISCs to be realized.

Description

technical field

[0001] The invention relates to a method for inducing human amniotic mesenchymal stem cells to differentiate into insulin-secreting cells in vitro. Background technique

[0002] Human amniotic mesenchymal stem cells (hAMSCs) have the advantages of multilineage differentiation, low immunogenicity, abundant sources, easy enrichment, strong expansion ability, and no medical ethical issues involved. It has broad application prospects. It has been proved that hAMSCs can differentiate into endothelial and hepatic epithelial cells, cardiomyocyte-like cells, neuron-like cells, osteoblasts, etc. under appropriate induction conditions, but whether they can differentiate into insulin-secreting pancreatic B cells has not been found so far. introduce.

[0003] The occurrence of type 1 diabetes is related to genetic factors, environmental factors and immune mechanism, and its fundamental etiology and pathogenesis is insufficient insulin secretion caused by the destructio...