New use of total glucosides of paeony and medicinal composition thereof
A technology of total glucosides of paeony and its composition, applied in the field of new uses of total glucosides of paeony and its pharmaceutical composition, to achieve the effects of increasing serum DA content, improving intestinal sensitivity, and inhibiting abdominal pain
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Embodiment 1
[0040] Example 1 Preparation of Total Glycosides of Paeony
[0041] Take the coarse powder of Radix Paeoniae Alba, use 70% V / V ethanol as the extraction solvent, the solvent dosage is 8 times the amount w / v, reflux extraction for 1.5h, and extract 3 times in total. Filtrate, combine the filtrates, concentrate under reduced pressure until there is no alcohol smell, add an appropriate amount of water to dissolve, centrifuge, take the supernatant, adjust its concentration to 1g / ml (containing 1g of Radix Paeoniae Alba in every 1ml of the medicinal solution), and the medicinal solution passes through a diameter of 1g. AB-8 macroporous adsorption resin column with a ratio of 1:20 (the volume ratio of medicinal solution to macroporous absorbent resin is 1:2). After the medicinal solution is adsorbed on the resin column for 30 minutes, wash away impurities with 4BV distilled water and collect 6BV60% V / V ethanol eluent, the flow rate is 3BV / h, the ethanol is recovered under reduced pr...
Embodiment 2
[0045] The preparation method of embodiment 2 total saponins of Glycyrrhizae
[0046] (1) Take licorice coarse powder, percolate with 20 times the amount of 95% ethanol, filter the percolate, concentrate under reduced pressure at 70°C to form a thick paste, stir and dissolve with heated water, and then use petroleum ether, ethyl acetate, water Saturated n-butanol extraction, the part obtained from ethyl acetate is the total flavonoids, and the n-butanol extract is used for later use.
[0047] (2) Take the filter residue obtained in step (1), percolate with 20 times the amount of 20% V / V ethanol, after the percolation liquid is filtered, the extract is concentrated under reduced pressure at 60-70°C until it has no alcohol smell, and then saturated with n-butyl Alcohol extraction, the obtained extract is combined with the n-butanol extract obtained in step (1), after the n-butanol is recovered under reduced pressure, the residue is dissolved in water, centrifuged, the supernatan...
Embodiment 3
[0051] Embodiment 3 Preparation of the pharmaceutical composition of the present invention
[0052] Take 100 g of total glucosides of paeony prepared in Example 1 and 300 g of total saponins of glycyrrhizin prepared in Example 2, add appropriate amount of microcrystalline cellulose, mix well, pack into capsules, and obtain.
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