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Method for separating and purifying epigallocatechin (EGC) monomer

A technology for separation and purification of epigallocatechin, applied in the food field, can solve problems such as the separation and purification of EGC monomers, and achieve the effects of good solubility, high product purity, and high monomer extraction rate

Active Publication Date: 2012-12-12
湖北宇隆生物工程有限责任公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report on the separation and purification of EGC monomer at home and abroad.

Method used

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Comparison scheme
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Embodiment Construction

[0016] The present invention will be further clarified through the detailed description of specific embodiments below, but it is not intended to limit the present invention, but only for illustration.

[0017] A method for isolating and purifying epigallocatechin monomer from green tea extract, the method comprises the following steps:

[0018] 1. Using tannase to convert epigallocatechin gallate (EGCG) in green tea extract into epigallocatechin (EGC):

[0019] (1) Add pure water to dissolve 95% tea polyphenols of green tea extract (95% tea polyphenols were purchased from Changsha Sanfu Biotechnology Co., Ltd., the total catechin content is 76.50%, the EGC content is 5.60%, and the EGCG content is 43.80%), configured as a solution with a concentration of 5% (w / v), added citric acid to adjust the pH = 4.20, and kept the temperature at 37 + 1°C, the tea soup stock solution is set aside;

[0020](2) Add tannase according to the weight of 0.02% (w / v) of the tea soup liquid, let ...

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Abstract

The invention discloses a method for separating and purifying epigallocatechin (EGC) monomer from a green tea extract. The method comprises the following steps: taking the green tea extract as a raw material, carrying out enzymatic hydrolysis on tannase, adsorbing resin column chromatography and eluting by hydrous ethanol, collecting epigallocatechin (EGC)-rich for fraction desorption, carrying out high efficiency external circulation concentration and recovering ethanol, using a reverse osmosis membrane for cryoconcentration, carrying out crystallization treatment, finally freezing and drying to obtain the epigallocatechin (EGC) monomer with high purity. The invention has the advantages of simple method, high monomer recovery rate and product purity, short preparation period and low cost, and is suitable for scaled industrial production.

Description

technical field [0001] The invention relates to a method for separating and purifying epigallocatechin (EGC) monomer from green tea extract, belonging to the technical field of food. Background technique [0002] Catechins have obvious pharmacological functions such as anti-aging, anti-cancer and anti-mutation, fat loss and weight loss, lowering blood sugar, blood lipid and cholesterol, and preventing cardiovascular diseases. They have been widely used in food processing, medical care, and daily chemical industries. (Wang Jing, Gao Yutang. Epidemiological research on anti-cancer effects of tea polyphenols[J]. Tumor, 2011, 31(6): 553-556; Liu Xueming, Liang Shizhong. Health care and pharmacological effects and application prospects of tea polyphenols[J]. J]. Food and Fermentation Industry, 1998, 24(5):47-51,71; Yokozawa Tx, Dong Ex, Nakagawa Tx, el al. In vitro and in vivo studies on the radical scavenging activity of tea [J]. J Agric Food Chem, 1998, 46(6):2143-2150). In O...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P17/06C07D311/62
Inventor 张盛刘仲华肖文军刘爱玲
Owner 湖北宇隆生物工程有限责任公司