Automated instrument for nucleic acid extraction and mass spectrum sample application

A nucleic acid and mass spectrometry technology, applied in the field of automated instruments, can solve the problems of ineffective large-scale nucleic acid extraction, low nucleic acid extraction efficiency, and time-consuming

Active Publication Date: 2012-06-13
BIOYONG TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

That is to say, the use characteristics and requirements of various biological instruments determine that it is impossible to achieve fast, direct and continuous experimental operations in the study of nucleic acid structure and function.
[0021]At present, semi-automatic or even manual methods are mainly used to extract nucleic acids in China. Manual methods are not only time-consuming and cumbersome, but also have low nucleic acid extraction efficiency and cannot be effectively carried out. Large-scale, large-scale nucleic acid extraction and purification analysis

Method used

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  • Automated instrument for nucleic acid extraction and mass spectrum sample application
  • Automated instrument for nucleic acid extraction and mass spectrum sample application
  • Automated instrument for nucleic acid extraction and mass spectrum sample application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] Example 1: Extraction and separation of nucleic acid samples in body fluids (see Figure 3-5 )

[0070] 1 Add reagents and samples

[0071] In 28 96-type deep-well plates, add lysate, magnetic beads, salt washing solution, washing solution, washing solution, washing solution, and eluent in sequence, seal the 96-type deep-well plate with a sealing film, and place it in the moderate environment. Wherein said lysate, magnetic beads, salt washing solution, washing solution, and eluent can use the reagents in the known magnetic bead DNA extraction kit, such as the 96-well magnetic bead tissue DNA of Shanghai Gongshuo Biotechnology Co., Ltd. Extraction Kit (Cat. No. M6229-00), Magnetic Bead Blood DNA Extraction Kit (Cat. No. M6221-01) from Shanghai Yixin Biotechnology Co., Ltd.

[0072] Divide the 7 96-type deep-well plates that have been added with corresponding reagents into 4 layers in the order of lysate-magnetic beads-salt wash-wash solution-wash solution-wash solutio...

Embodiment 2

[0078] Embodiment 2: The nucleic acid pipetting and dispensing device controlled by computer automation (see Figure 6 )

[0079] 1 Cold storage of nucleic acids

[0080] When the 96-type deep-well plate 251 containing the extracted nucleic acid transported by the nucleic acid extraction device enters the existing flat plate carrier 28 in the nucleic acid pipetting distribution device, and then is transported into the cold room 27 by the bottom moving device along the guide rail 21;

[0081] 2 Reagent addition and nucleic acid movement

[0082] After the nucleic acid extraction of four 96-control deep-well plates is completed in sequence, they are transported out from the cold room 27 to the level below the 96-channel automatic pipette 22 . Driven by the three-dimensional moving part 26, the automatic pipette 22 absorbs the tips and reagents in the pipetting tip box (right) 231 and the reagent box (left) 232, then adds the reagents and moves the nucleic acid to the 384-we...

Embodiment 3

[0085] Embodiment three: Chip sample spotting (referring to Figure 7-9 )

[0086] Open the water inlet valve, inject the ultrapure water into the ultrapure water cleaning tank 323 through the water inlet pipe of the water inlet, and close the water inlet valve after the water inlet process is finished.

[0087] Before sampling, inject 100% alcohol solution in the alcohol bottle 321, install the alcohol bottle 321 upside down so that its 100% alcohol solution flows to the ultrasonic cleaning tank 322 and fill the cleaning tank, and place the sampling needle 31 (preferably 24 needle metal drip liquid head) into the ultrasonic cleaning tank 322 containing alcohol, and soak for 30 minutes to clean the sample remaining on the tip of the sampling needle 31.

[0088] According to the different concentration of the sample, select the appropriate speed, depth, time, speed, depth and time of the liquid, and save the set parameters and methods.

[0089] At the beginning of sampling, t...

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Abstract

The invention discloses an automated instrument applied to nucleic acid extraction and continuous operation process of a mass spectrum sample application chip in biomass spectrometry analysis of a nucleic acid sample. The automated instrument comprises three main devices, namely, a nucleic acid extraction and purification device, a nucleic acid liquor relief distributing device and a mass spectrum chip sample application device, wherein nucleic acid in body fluid is extracted by using a magnetic bead method, a mechanical arm is utilized to complete a nucleic acid extraction action, the extracted nucleic acid is dispensed onto a sample board through a dispenser, a pipettor is used for transferring the sample board onto a chip sample application instrument, and the mass spectrum and relatedinformation of the nucleic acid are obtained through laser sampling of a mass spectrometer, so that the nucleic acid is analyzed rapidly, directly and continuously. The automated instrument is high-precision in controlling the volume of dispensed liquor, high-cleanliness in controlling the quality of the dispensed liquor and high-efficiency in meeting sample application requirements of large-batch samples; meanwhile, the samples are uniformly distributed under the control of a computer program, volume errors and possible foreign pollution caused by manual operation are avoided, and the speed and efficiency in sample application are increased at the same time.

Description

technical field [0001] The invention relates to an automatic instrument for extracting nucleic acid and spotting a chip. The automatic instrument for extracting nucleic acid in body fluid by using a magnetic bead method and realizing mass spectrometry chip spotting can be used to prepare a biochip, and thus belongs to the field of nucleic acid detection. Background technique [0002] Nucleic acid research is an important topic in modern biology and medical research. As the carrier of genetic information, nucleic acid is the material basis of gene expression. In addition to playing a very important role in the normal growth, development, and reproduction of organisms, it is also related to abnormal conditions of life, such as tumor occurrence, radiation, etc. Injuries and genetic diseases are also closely related. Nearly 2,000 genetic diseases have been found to be related to DNA structure. For example, human sickle cell anemia is due to a change in the genetic code of an ami...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12M1/34C12Q1/68G01N27/62
Inventor 马庆伟邢双艳时刊
Owner BIOYONG TECH
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